Okolab CO2-O2-UNIT-BL Manual Download Page 58

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Troubleshooting

We have collected in the table below some frequently asked questions, please contact Okolab if you need

support.

Symptom

Probable cause

Remedy

No power supplied to Unit

Power plug is disconnected

Connect plug securely

No Gas flow rate stability

Pressure drop

Check gas tanks and/or compressed lines

Change the PTFE filter with degree of
filtration 0.2 micron

No CO2 or O2 read

The Unit is off

Switch the Touch Screen on

The PTFE filter with degree of
filtration 0.2 micron is dirty

Change the PTFE filter with degree of
filtration 0.2 micron

Gas leak on the rear of the control box

Not properly inserted tubes

Strongly push the tube into the push to fit
connector

No Gas arrives to the incubating chamber

Missing connection

Make sure you have attached the TUBE-CB
on the rear panel of the unit.

Gas source closed

Open gas tanks regulators

Fast sample drying

No Gas arrives to the incubating
chamber

See above

Humidifying Module empty

Fill it with distilled water until 50 mm below
the top of glass column.

Humidifying Module not heated

Correctly connect the heater of the
Humidifying Module

Summary of Contents for CO2-O2-UNIT-BL

Page 1: ...CO2 O2 UNIT BL 0 10 0 1 Gas Controller Manual IST2180_REV01 SV 2 2 5 ...

Page 2: ...This page was left Blank ...

Page 3: ...sors with calibration gas 27 9 2 1 2 2 1 View 28 9 2 1 2 2 2 Span Settings 28 9 2 1 2 2 3 Factory Reset 29 9 2 1 2 2 4 Start Span 29 9 2 1 2 2 5 Start Zero 30 9 2 1 2 3 Factory Reset 31 9 2 1 3 Flowrates 31 9 2 1 4 Gas cycles 33 9 2 1 5 Status 36 9 2 2 Touch Screen configuration 37 9 2 2 1 Touch Screen Options 37 9 2 2 2 Touch Screen Brightness 37 9 2 2 3 Touch Screen Visual Effects icon and glanc...

Page 4: ...16 FIGURE LIST 55 17 MANUAL REVISION TABLE 56 ...

Page 5: ...load In addition data logging is possible via SMART BOX via DATA LOG Okolab software and via following third party Imaging software LASX 2 0 NIS Elements SlideBook MetaMorph and MicroManager Macro for ZEN Black software is available upon request Okolab Bold Line controllers are also capable to receive TTL input requires to order separately TTL CABLE CO2 O2 UNIT BL 0 10 0 1 connects to any Okolab B...

Page 6: ...rument Tip Supplies you with helpful suggestions Note Supplies you with important information to successfully use the instrument Symbols on the product label CE MARKING this symbol indicates product compliance with EU legislation PRODUCT DISPOSAL this symbol indicates that this product must not be disposed as urban solid waste This symbol indicates the product production date This symbol indicates...

Page 7: ...ny gas stream whose composition is different from that of ambient air Prevent throttling and kinking of tubing Check tubing time to time for possible material usage Check that all tubing ends are well inserted into the connectors so they cannot slip off This device is not designed for use for medical applications Install safety valves and adequate pressure regulators on gas lines before CO2 O2 UNI...

Page 8: ...here it can fall or be pushed into water International caution symbol marks this device It is important to read the Safety Notes before installing using and commissioning this device as the notes contain important information relating to safety and EMC Not following these instructions can result in damage or breakdown of the device and its accessories We reserve the right to make technical variati...

Page 9: ...e 4mm ID plus PTFE filter in the middle connected to a Polyurethane 120 cm long blue tube 6mm OD Use TUBE B to connect the CO2 O2 UNIT BL 0 10 0 1 to the cage incubator 4 TUBE A x3 Rigid tubes Polyurethane 3m long 6mm OD each Use TUBE A to connect N2 Air and CO2 supply to the corresponding input ports N2 Air and CO2 on the rear panel of the CO2 O2 UNIT BL 0 10 0 1 5 Pressure Gauge for CO2 N2 regul...

Page 10: ...g 20 3 psig with a connector for 6mm OD rigid tube Note An alternative to the compressed air OKO AIR PUMP BL allows to use background Air 6 Compatibility CO2 O2 UNIT BL 0 10 0 1 is compatible with OKO TOUCH required including Touch Screen Interface 1 RS232 Serial cable To connect the Touch Screen to the unit MINI USB OTG cable To connect a USB pen to the OKO TOUCH for data download OKO AIR PUMP BL...

Page 11: ...m a PC It may be used to change parameters of the controllers via PC Data can be exported in xlsx format Figure 3 DATA LOG software SM BL Smart Box To store data to operate the controllers via web smartphone tablet and to allow remote assistance for service ...

Page 12: ...y mixed gas to the user system 4 Power Input 5 OKO AIR PUMP BL connector Leave unplugged if CO2 O2 UNIT BL 0 10 0 1 is not equipped with OKO AIR PUMP BL 6 HM ACTIVE connector Leave unplugged if CO2 O2 UNIT BL 0 10 0 1 is not equipped with HM ACTIVE 7 GPIO General Purpose Input Output connector 8 USB connector 9 N2 Input Push to fit Input connector for 6 mm O D rigid tubing to supply N2 to the mixe...

Page 13: ...anel 1 Oxygen Analyzer includes the O2 sensor 2 Oxygen Analyzer Gas input connector Push to fit connector 4 mm OD rigid tube for mixed gas input to O2 Sensor 3 Oxygen Analyzer Gas output connector Push to fit connector 4 mm OD rigid tube for mixed gas output from O2 Sensor 1 2 3 Figure 6 Gas Controller Left Panel Overview ...

Page 14: ...supplied pressure gauge between the N2 supply and CO2 O2 UNIT BL 0 10 0 1 Follow the arrow on the rear of the pressure gauge for the correct Gas In Out see Figure 7 GAS IN GAS OUT Figure 7 Input and Output gas ports See symbol on the rear of pressure gauge On the bottom of the pressure gauge there is an automatic purging valve Close it before operating the pressure gauge To close the purging valve...

Page 15: ...t enters in contact with CO2 O2 UNIT BL 0 10 0 1 gas sensors Connect the TUBE CB with the Filter In and Filter Out connectors on the rear panel of CO2 O2 UNIT BL 0 10 0 1 1 2 in Figure 5 and Figure 10 Make sure the Filter In label side of the filter is always connected to Filter In connector on the rear panel of CO2 O2 UNIT BL 0 10 0 1 Note CO2 O2 UNIT BL 0 10 0 1 cannot operate if the TUBE CB is ...

Page 16: ... 1 make sure there is no residual pressure by adjusting the pressure gauges upstream of the system and the tanks 4 To install the O2 Sensor follow the instructions below Carefully open the bag of the O2 Sensor using a pair of scissors or a knife Make sure that there is no sign of any liquid in the bag if so do not proceed you need a new sensor Be careful that you don t poke anything such as a fing...

Page 17: ...input connector and Oxygen Analyzer Gas output connector see image 5 in Figure 12 Make sure that the Oxygen analyzer tubes are securely connected Tip Make sure to push the tubes all the way into the connectors thus avoiding any gas leak 1 2 3 4 5 6 O2 SENSOR ORING Figure 12 O2 Sensor installation 5 Figure 13 shows the connections between the N2 Air and CO2 tanks and the respective connectors on th...

Page 18: ... N2 Air and CO2 tanks connections to the back panel of CO2 O2 UNIT BL 0 10 0 1 Tip If you have ordered OKO AIR PUMP BL connect TUBE B between the OKO AIR PUMP BL and the connector labeled Air on the rear panel of CO2 O2 UNIT BL 0 10 0 1 see Figure 14 ...

Page 19: ...l of CO2 O2 UNIT BL 0 10 0 1 6 Connect one end of the TUBE C to the push to fit connector of the port labeled Gas output on the rear panel of CO2 O2 UNIT BL 0 10 0 1 and the other side of the TUBE C to your device as shown in Figure 15 TUBE C Gas Input of your device C Figure 15 Gas Output connections ...

Page 20: ... connect the CO2 O2 UNIT BL 0 10 0 1 gas output to the to the port labeled Gas Input on the Humidity Sensor Lid of HM ACTIVE see Figure 17 Connect the Heated Based cable of HM ACTIVE to the port labeled H M on the rear panel of CO2 O2 UNIT BL 0 10 0 1 see Figure 17 HM ACTIVE CO2 O2 UNIT BL 0 10 0 1 TUBE C Figure 17 Gas Output connections with HM ACTIVE Note If you purchased CO2 O2 UNIT BL 0 10 0 1...

Page 21: ...9 To switch CO2 O2 UNIT BL 0 10 0 1 on use the power button on OKO TOUCH The Status LED 1 in Figure 4 on the front of CO2 O2 UNIT BL 0 10 0 1 will turn green 10 Once the system has been initialized the Status LED on the front of CO2 O2 UNIT BL 0 10 0 1 will stop blinking and will turn steadily on The Homepage will appear on the touch screen the color of the Status Indicator see 1 in Figure 22 and ...

Page 22: ...1 for example with a H301 T UNIT BL PLUS Figure 20 CO2 O2 UNIT BL 0 10 0 1 and H301 T UNIT BL PLUS CO2 O2 UNIT BL 0 10 0 1 must stack on top of the Bold Line T controller by lining up the bus ports located on the top and bottom surface of each unit When the units are properly connected the Temperature and CO2 O2 parameters will appear on the OKO TOUCH Touch Screen interface Home page Tip If not al...

Page 23: ... BL PLUS on stock If you have CO2 O2 UNIT BL 0 10 0 1 and a Bold Line T controller it is essential to read both this manual and the Bold Line T controller User Manual to familiarize yourself with the functions and the operation of the devices before use ...

Page 24: ...in the time frame set in the Options subpage see paragraph 9 2 2 1 5 Chart history length set at 5 minutes 6 Display mode Touch here to switch display mode 7 Overview page 8 Settings Press here to access system options and settings 9 Home To open the homepage 10 Product info Press here to know generic info about CO2 O2 UNIT BL 0 20 1 95 and running time Tip The OKO TOUCH is pre set at 6 CO2 and 1 ...

Page 25: ...e Setpoint by clicking on and Once you have input the new CO2 or O2 Setpoint press Set 2 in Figure 23 b and 2 Figure 24 b to save or Cancel to undo Tip After any change in the Setpoint value CO2 O2 UNIT BL 0 10 0 1 enters into a transient regime the Status Indicator and the TOP LED see Figure 25 turn yellow see paragraph 9 1 2 During the transient regime CO2 O2 UNIT BL 0 10 0 1 will not trigger on...

Page 26: ...ition values within the maximum time defined by the operator see paragraph 9 2 2 5 the Gas Controller Status changes to ALARM and the color turns ORANGE Controller Status TRANSIENT The ORANGE color indicates that the current gas concentration is not correct and its value is out of the set tolerance see section ALARMS in paragraph 9 2 2 5 Most commonly this is related to gas leaks or gas source s r...

Page 27: ...Settings Submenu press the Gas Icon see Figure 26 b Then the page shown in Figure 27 appears Figure 27 Gas Settings submenu 9 2 1 1 Air Source Press the icon to set which Air Source you are using see Figure 28 a If this is an external Air tank or compressed Air line Compressed Air or if you are instead using OKO AIR PUMP BL Air Pump See Figure 28 b ...

Page 28: ...in Figure 29 b a b 1 2 Figure 29 How to select Air Pump a b Note When using Air Pump the maximum total flow is limited to 0 4 l min To connect the Air Pump OKO AIR PUMP BL to CO2 O2 UNIT BL 0 10 0 1 see Figure 14 In the case you are using OKO AIR PUMP BL please note that it can be turned on off only via the Touch Screen interface OKO TOUCH Thus it is essential that you hereby set the correct Air s...

Page 29: ...n external CO2 O2 meter NOT provided Figure 31 How to enter a gas offset calibration Please make sure that the external Gas meter used is accurate and calibrated in the range near your desired setpoint To run the gas sensor calibration using an external gas meter follow the indications below 1 It is necessary to attach the external gas meter to the connector Gas Output on the rear of the CO2 O2 Un...

Page 30: ... icons to input the CO2 1 in Figure 33 b and O2 2 in Figure 33 b levels read by the external meter i e 5 0 for CO2 and 0 51 for O2 then press Save to continue 3 in Figure 33 b a b 3 2 1 Figure 33 Offset Calibration start a b Note The Touch Screen will go back to the previous screen It will display on the left External Probe tab the CO2 and O2 levels read by the external Gas meter as just input The...

Page 31: ...ill delete all custom calibration and restore the factory values see Figure 35 Figure 36 Restore the factory values 9 2 1 2 2 Sensors with calibration gas This function allows performing a calibration using a Span Gas or pure N2 Tank Before starting the instrument calibration make sure you have the Gas Tank Span Gas or N2 tank connected to the port labeled N2 on the rear panel of CO2 O2 UNIT BL 0 ...

Page 32: ...ration status a b 9 2 1 2 2 2 Span Settings Press on Span Settings icon to access to the span gas settings see Figure 39 a Press on icons to input the Concentration see 1 in Figure 39 b and the Deviation see 2 in Figure 39 b of your span gas tank as reported in span gas tank s certificate Press Save to confirm see 3 in Figure 39 b a b 1 2 3 Figure 39 Span Settings Menu a b ...

Page 33: ... Factory Reset Menu a b 9 2 1 2 2 4 Start Span Press the Start Span icon and the controller will perform a new Gas Calibration for the CO2 and O2 using a span gas see Figure 41 a After clicking a pop up message will appear warning about the pause of the control during the calibration routine see Figure 41 b The controller will ask for connecting the span tank at 1 4 barg to the N2 Input located on...

Page 34: ...hown in Figure 43 b a b Figure 43 Span calibration running a b 9 2 1 2 2 5 Start Zero Press the Start Zero icon to perform a new Zero Calibration see Figure 44 a Note Okolab recommends to use N2 with Standard Purity coded as 5 0 After clicking a pop up message will appear warning about the pause of the control during the calibration routine see Figure 44 b ...

Page 35: ...rmed or if for any reason you want to reset to the factory calibration press on Factory Reset icon see Figure 46 a A pop up message appears see Figure 46 b Press Reset to proceed or Cancel to undo a b Figure 46 Sensor calibration factory reset a b 9 2 1 3 Flowrates Press the Flowrates icon to check how many liters per minute of N2 CO2 and Air the system is consuming see Figure 47 a Additionally th...

Page 36: ...te When setting the flowrate for values below 0 2 l min the warning message Total Flow not allowed appears see Figure 49 Figure 49 Flowrates for values below 0 2 l min Note You can set the flowrate at 0 0 l min in this case the gas flowrate will be paused and output flow will be interrupted see Figure 50 In this working mode three dots will appear in Homepage instead of CO2 and O2 current value an...

Page 37: ...a b You can decide whether setting a new gas cycle New as shown in Figure 51 b or load a previously saved one Load To set a new CO2 and O22 concentrations cycle follow the indications below 4 Choose the two CO2 and O2 concentrations between which you want to switch SP1 Setpoint 1 and SP2 Setpoint 2 as shown in Figure 52 5 Set the duration in hours and minutes you want the system to dwell on each c...

Page 38: ...mary page will be displayed see Figure 54 a Press Save As if you want to save the CO2 O2 Cycle program to be recalled for future experiments Note You can save up to 4 different CO2 O2 Cycles programs Saving a 5th one will overwrite the first one 1 2 a b Figure 54 Cycle Summary and Configuration Saving a b 8 Schedule the time for the concentration cycle to start see Figure 55 ...

Page 39: ... Setpoint or starting a new cycle while a cycle is still running will open the window in Figure 57 You can press Abort to abort the cycle Pressing View will open the current cycle settings Press Home to undo Figure 57 Warning shown if you try to change Setpoint during a gas cycle Note The images shown in this paragraph are for illustrative purpose only They are not intended to reflect the touch sc...

Page 40: ...con to check CO2 O2 UNIT BL 0 10 0 1 performance see Figure 58 a a b Figure 58 Status page a b Note The Status page contains advanced technical data You may be asked to read these data in case of technical assistance with Okolab engineers ...

Page 41: ...s or scroll the Chart history length bar 1 in Figure 60 b Note The chart history value length is pre set at 5 minutes Note Recommended value for the chart history length is 60 or 120 minutes To change the sound frequency press the icons or scroll the Buzzer frequency bar 2 in Figure 60 b then press Save 4 in Figure 60 b to confirm Note To activate a sound at each touch flag the Touch Buzzer checkb...

Page 42: ...ing see also paragraph 9 4 The Glance mode setting defines the colour of the TOP LED and of the digits displayed in Glance Mode If you select White the TOP LED illuminates in white colour according to the criterion selected in TOP LED setting and the digits displayed in Glance Mode are white If you select Dynamic the colour of the TOP LED and the colour of the digits in Glance Mode follow the colo...

Page 43: ... Buzzer checkbox if you want the Alarm to be acoustical as well rather than just being displayed a b Figure 64 How to enter in Alarms page a b 2 Insert the Gas Deviation by scrolling the bar 1 in Figure 65 or pressing the icons Note The Gas Deviation value defines the tolerance from the Setpoint Note The gas deviation value range is 0 1 2 0 3 Insert the Gas Time by scrolling the bar 2 in Figure 65...

Page 44: ...nge in the Setpoint value the device enters into a transient regime 9 2 2 6 Data Logging CO2 O2 UNIT BL 0 10 0 1 touch screen interface OKO TOUCH see Figure 2 is equipped with on board memory for data logging and storage A simple routine allows downloading data to USB drive or to PC In order to log and then download the logged data connect a USB drive not supplied to OKO TOUCH using MINI USB OTG c...

Page 45: ... page also by pressing the activated logging icon on the Homepage as shown in Figure 69 a b Figure 68 Logging a How to enter in the logging menu b logging in internal memory Figure 69 How to access the Logging page from the Homepage 3 Press on the icon to insert the Time Interval i e the frequency with which you want to record a data point as shown in Figure 70 In this example a data point is logg...

Page 46: ...ite the file name suffix to be reported in the downloaded files by pressing on the keyboard icon see Figure 71 a 6 Press Save see Figure 71 b Tip With the selections as in Figure 70 and in Figure 71 the data will be downloaded on the USB drive as shown in Figure 72 a b Figure 71 Data Logging How to define the suffix for the downloaded files a b Figure 72 Downloaded files on the pen drive Note If y...

Page 47: ... the data press on To USB or on Erase if you want to delete the data see Figure 73 b a b Figure 74 Download to USB You can activate the logging also on the USB drive by flagging on USB drive see Figure 75 a a b Figure 75 Logging on USB flash drive a USB drive selection b Reminder to connect the USB drive In this case OKO TOUCH warns you that a USB drive must be connected to the USB port see Figure...

Page 48: ...e icon to open the Overview page as show in Figure 77 a a b Figure 77 Overview Status page a How to enter in the Overview Page b Overview Status Page Note This page contains data useful for technical control reasons In case you requested support from one of Okolab engineers you may be asked for some of these data Icon and Glance Mode View CO2 O2 UNIT BL 0 10 0 1 features two display modalities Ico...

Page 49: ...gure 79 This page contains the information related to the OKO TOUCH as shown in Figure 80 a and to CO2 O2 UNIT BL 0 10 0 1 version as shown in Figure 80 b Figure 79 Info page selection a b Figure 80 Info page OKO Touch a and CO2 O2 UNIT BL 0 10 0 1 a b Tip Please have this information handy when contacting Okolab for support ...

Page 50: ...t the Touch Screen Calibration see Figure 81 While holding the button the pop up message shown in Figure 82 a appears Then tap blue calibration dots until the calibration is complete see Figure 82 b Figure 81 How to enter in Touch Screen Calibration a b Figure 82 Start Touch Screen Calibration a b ...

Page 51: ...oses tubing If some hoses tubing is damaged contact Okolab to receive assistance After 2 years disconnect all polyurethane tubing cut the last 1 cm of the tubing and reconnect them Replace the PTFE filter on the rear panel of the unit and along the tubes each 2 3 months of continuous use or when it is present water in the inner part of the filter Okolab Air pumps have to be replaced every 18 24 mo...

Page 52: ...mount it close to an outside door or air conditioning duct that will allow a sudden draft of cold or hot air to blow on it Maintenance The oxygen probe is virtually maintenance free other than for periodic calibration and periodical sensor replacement Periodic Calibration The probe system should be calibrated about once every three weeks to obtain the best accuracy The sensor typically declines in...

Page 53: ...t be thrown into ordinary trash Refer to the MSDS to learn about potential hazards and corrective actions in case of any accident Figure 83 O2 Sensor O2 Sensor Replacement Procedure The O2 Sensor is provided in a special sealed bag Do not open this until you are totally ready to install the sensor 1 Disconnect the two tubes from the Oxygen Analyzer Gas input connector and Oxygen Analyzer has outpu...

Page 54: ... new sensor Be careful that you don t poke anything such as a fingernail through the membrane 6 Remove the plug or other device that acts as a shorting clip This may be found on the connection plate on the back of the sensor 7 Slide the sensor into the sensor unit gold plated contact side of sensor should be facing up touching the sensor unit contacts The membrane side is covered by a convex gold ...

Page 55: ...he cap making sure that you do not cross thread it and tighten firmly by hand Do not over tighten 10 Allow the sensor to stabilize for a few minutes and then calibrate it preferably using known fresh air as the calibration gas ...

Page 56: ...o contact Okolab should you need any further commercial information or technical support For HARDWARE SUPPORT sibillo oko lab com Phone 39 081 806 3470 Fax 39 081 876 4410 Mobile 39 348 96807 18 For COMMERCIAL SUPPORT WORLDWIDE lanzaro oko lab com Phone 39 081 806 2624 Fax 39 081 876 4410 Mobile 39 348 96807 17 For COMMERCIAL SUPPORT US CANADA foppiano oko lab com For COMMERCIAL SUPPORT CHINA tong...

Page 57: ...Range 0 1 Step size 0 1 Accuracy 0 02 1 0 Sensor 1 year life electrochemical sensor Recommended Calibration Interval three weeks Input Gas CO2 N2 and Air Total Flow rate 0 2 1 l min Operating Temperature 0 C 55 C 23 C suggested Storage Temperature 5 C 60 C Operating Humidity 0 70 Power Input Input 24 V DC 35 W max 85 W max if used together with HM ACTIVE Dimensions LxWxH in mm 200x200x121 50 Weigh...

Page 58: ...er with degree of filtration 0 2 micron is dirty Change the PTFE filter with degree of filtration 0 2 micron Gas leak on the rear of the control box Not properly inserted tubes Strongly push the tube into the push to fit connector No Gas arrives to the incubating chamber Missing connection Make sure you have attached the TUBE CB on the rear panel of the unit Gas source closed Open gas tanks regula...

Page 59: ... Source page a b 24 Figure 29 How to select Air Pump a b 24 Figure 30 How to reach the Sensor Calibration Submenu a b 25 Figure 31 How to enter a gas offset calibration 25 Figure 32 External Gas Meter Connection 26 Figure 33 Offset Calibration start a b 26 Figure 34 Offset Calibration Correction 27 Figure 35 Offset Calibration End 27 Figure 36 Restore the factory values 27 Figure 37 How to insert ...

Page 60: ...e 74 Download to USB 43 Figure 75 Logging on USB flash drive a USB drive selection b Reminder to connect the USB drive 43 Figure 76 Logging to USB drive 44 Figure 77 Overview Status page a How to enter in the Overview Page b Overview Status Page 44 Figure 78 Display modalities a Icon Mode b Glance Mode 45 Figure 79 Info page selection 45 Figure 80 Info page OKO Touch a and CO2 O2 UNIT BL 0 10 0 1 ...

Page 61: ...warranted Okolab S r l neither assumes responsibility for any omissions or errors nor assumes liability for any damage that may results from improper use of its products in accordance with information provided by Okolab S r l Okolab S r l warrants only the parts manufactured by Okolab S r l to be free of defects Okolab S r l makes no other warranties or representations of any kind whatsoever expre...

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