OM0133
3
DH Autoflow Air-Jacketed
Automatic CO
2
Incubator
Models:
NU-5500
NU-5500E
Operation and Maintenance Manual
1.0 General
Description
The NuAire DH Autoflow Automatic CO
2
Air-Jacketed Incubator has been designed to provide a reliable
controlled in-vitro environment for optimum tissue cell culture growth. The chamber also provides an
environment for the storage and preservation of embryos, gametes and animal tissue cell cultures at on near body
temperature. There are five parameters that contribute to optimum growth conditions. These are:
1. Humidity
2.
Precise temperature control
3. Precise
CO
2
control
4. Sterility
5. Reliability
Like all NuAire equipment, this incubator has been designed to provide the highest quality standards of
performance with matching computer technology, precise temperature control, and CO
2
gas control system
combining state-of-the-art technology with years of design, quality, and manufacturing experience.
In order to accomplish the foregoing objectives, this incubator features the following:
1.1 Incubator
Chamber
The design and size of the DH Autoflow inner chamber provides a large capacity, and ease of use. The
chamber walls are directly heated by physically attached foil heating elements on the sides, bottom, top,
and back of the chamber, providing a temperature uniformity of +0.3
C. A space-age high-density
insulation that has a high "R" rating covers the complete outer surfaces of the incubator inner chamber.
1.2
Incubator Blower Fan & HEPA Filter
A continuous operating fan motor drives a blower wheel within the upper air plenum and sidewall duct
system. Air is constantly re-circulating within the chamber keeping every cubic inch of volume at a
uniform temperature. This airflow is distributed uniformly and at very low velocity, so as not to influence
culture growth. A large replaceable HEPA filter cartridge continually filters the air that circulates in the
chamber.
1.3
Incubator Control Electronics
The NuAire Incubator Control Electronics is a state-of-the-art microcomputer based control system
specifically designed to service the precise control requirements of the chambers environment, providing
optimum programmable conditions for culture growth. The microcomputer is “user-friendly” with status
indicators, LED display of control parameters and five touch control keypads to permit efficient operator
entry of data.
The microcomputer is supported with Read Only Memory ROM) containing executable software,
Random Access Memory RAM) for temporary storage, and Electronically Erasable Programmable Read
Only Memory EEPROM) for control set points and parameters. The
EEPROM provides for indefinite storage of these values during periods of power off or power
interruption power fault tolerant). The microcomputer includes a complete internal diagnostic software
package that permits fault isolation detection down to the failed component.
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