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Page 1: ...xcess underutilized and idle equipment along with credit for buybacks and trade ins Custom engineering so your equipment works exactly as you specify Critical and expedited services Leasing Rentals Demos In stock Ready to ship TAR certified secure asset solutions Expert team I Trust guarantee I 100 satisfaction All trademarks brand names and brands appearing herein are the property of their respec...
Page 2: ...ance Manual October 2010 Revision 9 Series 14 For 115 Vac 60 Hz Only Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 Toll Free 1 800 328 3352 In Minnesota 763 553 1270 Fax 763 553 0459 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...
Page 3: ...meter Temperature Calibration 9 3 Setting Air Injections 9 4 CO2 Calibration Section No 10 Maintaining Your DH Autoflow Section No 11 Error Indicators Troubleshooting Section No 12 Remote Alarm Contacts Section No 13 Electrical Environmental Requirements Insert Replacement Parts List MANUAL DRAWINGS BCD 09441 NU 5500 E Direct Heat CO2 Incubator BCD 09409 Plenum Shelf Installation BCD 09439 Front C...
Page 4: ...ers the complete outer surfaces of the incubator inner chamber 1 2 Incubator Blower Fan HEPA Filter A continuous operating fan motor drives a blower wheel within the upper air plenum and sidewall duct system Air is constantly re circulating within the chamber keeping every cubic inch of volume at a uniform temperature This airflow is distributed uniformly and at very low velocity so as not to infl...
Page 5: ...ng crevice free construction which provides an easily cleanable inert surface that does not in itself promote biological growth In addition all shelves shelf supports guide rails and the air plenum are easily removable and can be autoclaved to remove contamination Remember The chamber is not selective The growth environment is applied equally to all microorganisms within the chamber 1 6 Incubator ...
Page 6: ...lly accessible from the front of the unit 2 9 A thru wall access port is provided for operating electrical appliances such as roller apparatus rockers etc 2 10 A CO2 sample port is provided on the front panel to check the concentration of CO2 in the chamber 2 11 The CO2 percentage is controlled by a solid state gas infrared sensor which provides accurate monitoring of CO2 regardless of changes in ...
Page 7: ... Display Resolution 0 1 Door and Perimeter Heater Control Logic Proportional 0 100 adjustable Door Perimeter Heater Duty cycles are processor controlled Temperature Sensor Type Precision Integrated Circuit CO2 Control Logic Fixed Algorithm Manual Environmental Adaptable CO2 Sensor Type Infrared Connectors on rear panel are provided for chart recorder remote alarm and printer connection NOTE RATING...
Page 8: ...el NU 1557 Additional Shelves Model NU 1559 CO2 Analyzer Fyrite Kit Dry 0 20 replacement fluid required Model NU 1561 Replacement Fluid for CO2 Analyzer two bottles carton Model NU 2568 Surge Protector 115 VAC Model NU 1564 CO2 Regulator 2 Stage Fyrite Replacement Fluid may only be ordered when shipment is possible by UPS Ground Service Artisan Technology Group Quality Instrumentation Guaranteed 8...
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Page 10: ...lts VDC ONLY is applied between dead metal parts and the hot neutral power source lead with no electrical breakdown using an Associated Research Model 4045AI or 3560D for 115 VAC units 2100 volts VDC ONLY is applied for 230 VAC units 4 2 3 Grounding Continuity The resistance between the green bonding conductor of the supply cord and any dead metal part of the cabinet shall not exceed 0 10 ohms 4 3...
Page 11: ...his number must accompany all returns Any product shipped to NuAire without this number will be returned refused shipment or collect freight 6 0 Shipments NuAire Inc takes every reasonable precaution to assure that your Incubator arrives without damage Motor carriers are carefully selected and shipping cartons have been specifically designed to insure your purchase However damage can occur in any ...
Page 12: ...maintenance service purposes the control center electronics should remain accessible 7 2 Leveling Prior to use the incubator should be leveled using a bubble level on a middle shelf in the chamber The Incubator should have all 4 feet firmly on the bench or floor Level from side to side and front to back Leveling feet are provided for this purpose factory installed into the base of the Incubator By...
Page 13: ...ated with CO2 gas only Connecting a flammable or toxic gas can result in a hazardous condition Gases other than CO2 should not be connected to this equipment CO2 gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve Check the gas cylinder for the proper identification labels Do not use CO2 gas cylinders equipped with siphon tubes A siphon tube is used to ex...
Page 14: ...nk 1 The filter should be inserted downstream of the low stage regulator before the inlet fitting on the Incubator as shown in the figure Secure each connection with the clamps supplied Observe proper flow orientation of the filter look for in or a green dot on the filter The tubing is easily cut with a sharp knife 7 5 5 CO2 Supply Adjustment With the regulator OFF i e fully counterclockwise open ...
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Page 16: ...mended to fill the pan to about 1 2 inch below the top rim See Sections 3 1 and 8 2 for more specifications and operational details 7 8 Stacking Units Refer to BCD 09404 in the ASSEMBLY Section Align the feet of the top Incubator with the indentations in the lid of the bottom Incubator Ensure that there is a minimum of 1 4 inch 6mm air gap between the Incubators as explained in section 7 2 to avoi...
Page 17: ...al free water no purer than 1 mega ohm in the stainless steel pan The water should be changed at least once a week preferably more often FLOODING THE BOTTOM OF THE INCUBATOR IS NOT RECOMMENDED since it is difficult to change the water weekly and almost necessitates the use of chemicals which are not recommended and may damage the stainless steel Also it promotes condensation on the inner walls bec...
Page 18: ...up is accomplished by depressing the UP or DOWN arrow key when the selected parameter is activated 8 3 4 Automatic notification of abnormal situations The red alarm LED on the control panel indicators will light to indicate a fault within the system An audible alarm is also heard System intermittent Temperature Control Fault Temperature is over setpoint by more than 1 0 C Temperature does not reac...
Page 19: ...ront control panel contains the following functions BCD 09439 8 4 1 Heat Jacket Status LED The green LED indicates when the chamber heater is turned on A blinking LED indicates the chamber heater is being cycled to maintain chamber setpoint temperature 8 4 2 Inject CO2 Status LED The green LED indicates when the CO2 control valve is open and CO2 is flowing into the chamber When it blinks on off br...
Page 20: ... Then again the temperature control will try to control 8 4 8 Chamber Sample The chamber sample port is provided to allow measurement of CO2 percentage manually with a fyrite CO2 indicator or other suitable instrument 8 4 9 Parameter Indicators LED The parameter indicators located next to the TEMPERATURE CO2 WINDOWS indicate the activated parameter being shown in the three digit display If the par...
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Page 23: ...Mode Operator Interactions The mode switch is used to select the mode of chamber operation setup or run The chamber operation mode is changed by pressing the run setup key for three seconds The mode switch LED will display a green light when in run mode In general there is no need for operator interaction in run mode However operator interaction is required to perform calibration functions and abn...
Page 24: ... manually and individually The diagnostic mode has three menus to select from that are the following 1 tst test output parameters 2 opt option configuration parameters 3 rst reset master To initiate the diagnostic mode perform the following In either run or setup mode press and hold the hidden key flag on NuAire logo for four seconds Temperature display will indicate the first menu test To advance...
Page 25: ...rn them all off and repeat the sequence until another function is selected 2 Safety Relay This function shows the current state of the safety relay The CO2 percent display will show yes or no corresponding to the relay condition The state can be changed by pressing UP DOWN 3 Chamber Temperature Sensor This function shows the current value of the chamber control temperature sensor on the CO2 displa...
Page 26: ...ion shows the current state of the door heater This function also allows the door heater to be turned on at different percentages 0 25 50 75 100 alternating with the dor indicator 9 Perimeter Heater This function shows the current state of the perimeter heater This function also allows the perimeter heater to be turned on at different percentages 0 25 50 75 100 alternating with the PEr indicator 1...
Page 27: ...values are in bold Once you have made your menu selection you will remain within that menu selection until you exit the diagnostic mode If another menu selection is desired you must re enter the diagnostic mode via the hidden key To exit the function parameters press the hidden key flag on NuAire logo 1 CO2 System Enable This function will enable or disable the CO2 system The value display will sh...
Page 28: ...larm condition will never come back after the user pushes a key to silence the audible alarm 6 Password Protection This function allows users to disable enable password to prevent unauthorized change of setpoint using the UP DOWN and SELECT keys combination Password requires three digits If password option is enable whenever SET UP key is pressed password will be required Every time password optio...
Page 29: ...creased to 100 to guard against condensation 10 Air Inject Time This value specifies the time in seconds for an injection of air into the chamber 11 Air Inject Cycle Time This value specifies the amount of time in minutes between each injection of air into the chamber 12 Print Frequency Time This parameter specifies the frequency in minutes that lines are to be printed on a status report If the fr...
Page 30: ...7 Temperature Maximum Above Setpoint This value determines the maximum deviation measured in temperature C that the chamber is permitted above or below once the incubator reaches the specified setpoint before an alarm condition is declared An alarm condition will cause the chamber to enter a safe condition where no power is enabled to any of the system output controls until the situation is rectif...
Page 31: ...toflow chamber Do not place the glass beaker on the bottom of the chamber because it will result in a slightly higher temperature reading due to the heater located on the chamber bottom Placing the thermometer in glass beaker on the middle shelf will give the most accurate results for calibration The chamber should be humidified to avoid false low readings due to evaporation from the flask An accu...
Page 32: ...eter duty cycles are set up when the room temperature is 22ºC and the room temperature is allowed to increase to 27º Less heat is required to keep the chamber at set point If the chamber starts to overheat the duty cycles will be reduced at a rate of 3 per minute starting when the chamber temperature is 0 2ºC above set point The duty cycles will continue to be reduced until the chamber temperature...
Page 33: ... stabilize Recheck and adjust the display if needed 9 4 2 CO2 Sensor Calibration Zero Span CAL INJ There are 2 sensor zero span calibration routines available to the lab professional An open door routine involving opening the outer and inner door to zero the sensor This routine also automatically calibrates to the CO2 injection rate during the injection for the span portion of the sensor calibrati...
Page 34: ...g Press the SEL key to indicate the LED next to the CO2 display Press the down until ZEr value alternate in the display Air is being pumped through the sensor to confirm the sensor zero value After 45 seconds the display is automatically zeroed and the span portion of the routine is started The CO2 display will show the following in order Span Calibration DLY alternating w value Shows for 90 secon...
Page 35: ...air is pumped through the sensor detector cell for 2 minutes The CO2 reading is checked at this time If it 0 5 or less different than the current zero the sensor will use the new value as zero When the value is greater than 0 5 the auto zero routine is aborted and an ACF alarm is sounded See Section 11 0 on Trouble Shooting for responses to this alarm The auto zero routine is scheduled to be initi...
Page 36: ...ed on one side of filter when replacing filter P N A 980899 01 Radial HEPA Filter Chamber HEPA Filter The chamber HEPA filter should be replaced approximately every two years to assure optimum performance A visual check should be performed periodically and during CO2 sensor calibration to assure filter integrity A visual check would include removing the plenum top and observing the HEPA filter int...
Page 37: ...perature sensor calibration 2 Check connection on control board 3 One or both temp sensors faulty replace CO2 System CO2 over setpoint 1 Perform CO2 sensor calibration 2 Check injection solenoid for leaking valve 3 Check sensor and disk filter for condensation CO2 time out error 1 Check CO2 gas supply inline gas filters CO2 gas tank pressure CO2 sensor function 2 Run Cal Inj Calibration see Sectio...
Page 38: ...hinge by loosening acorn nut on the cable clip 3 Check door switch if faulty replace Self diagnostic move 1 Indicates Incubator is performing self diagnostic task Calibration can be performed when task is completed When performing on off set calibration DLY shows in display and the value will not change 1 Indicates the Incubator is busy with an automatic function like an air injection Then display...
Page 39: ...n anything 3 Check jacket fan mounted in bottom on Incubator For further assistance call NuAire Customer Service at 1 800 328 3352 or 763 553 1270 USA 12 0 Remote Alarm Contacts The NuAire DH Autoflow contains a set of contact points to connect to a remote alarm system The contacts are located on the rear panel The contacts are housed in a modular RJ 11 telephone jack and rated for 30V at 1 Amp Th...
Page 40: ...that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation 13 6 Chemical Exposure Chemical exposure should be limited to antibacterial materials used for cleaning and disinfecting Chlorinated and Halogen materials are not recommended for use on stainless steel surfaces Chamber decontamination can be accomplished by paraforma...
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Page 54: ...quipment Have surplus equipment taking up shelf space We ll give it a new home Learn more Visit us at artisantg com for more info on price quotes drivers technical specifications manuals and documentation Artisan Scientific Corporation dba Artisan Technology Group is not an affiliate representative or authorized distributor for any manufacturer listed herein We re here to make your life easier How...
