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EXT-16DX User Manual 

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version 1.3 

 

 

page 12 

3. Literature 

 

Barmashenko, G., Eysel, U. T., & Mittmann, T. (2003). Changes in intracellular calcium 
transients and LTP in the surround of visual cortex lesions in rats. 

Brain Res.

 

990

, 120-

128. 

Boulton, A. A., Baker, G. B. & Vanderwolf, C. H. (eds.) (1990). 

Neurophysiological 

Techniques, Basic Methods and Concepts

. Humana Press, Clifton, New Jersey. 

Huemmeke, M., Eysel, U. T., & Mittmann, T. (2002). Metabotropic glutamate receptors 
mediate expression of LTP in slices of rat visual cortex. 

Eur.J.Neurosci.

 

15

, 1641-1645. 

Huemmeke, M., Eysel, U. T., & Mittmann, T. (2004). Lesion-induced enhancement of 
LTP in rat visual cortex is mediated by NMDA receptors containing the NR2B subunit. 

Physiol

 

559

 , 875-882. 

Schulz, D., Huston, J. P., Jezek, K., Haas, H. L., Roth-Harer, A., Selbach, O., & Luhmann, 
H. J. (2002). Water maze performance, exploratory activity, inhibitory avoidance and 
hippocampal plasticity in aged superior and inferior learners. 

Eur.J.Neurosci.

 

16

, 2175-

2185. 

Kettenmann, H. & Grantyn, R. (eds.) (1992). 

Practical Electrophysiological Methods

Wiley-Liss, New York 

Kowski, A. B., Veh, R. W., & Weiss, T. (2009). Dopaminergic activation excites rat lateral 
habenular neurons in vivo. 

Neuroscience.

 

161

, 1154-1165. 

Lalley, P.M., A.K. Moschovakis and U. Windhorst (1999) Electrical Activity of Individual 
Neurons In Situ: Extra- and Intracellular Recording, in: U. Windhorst and H. Johansson 
(eds.) Modern Techniques in Neuroscience Research, Springer, Berlin, New York. 

Lambert, F. M., Beck, J. C., Baker, R., & Straka, H. (2008). Semicircular canal size 
determines the developmental onset of angular vestibuloocular reflexes in larval Xenopus. 

Journal of Neuroscience

 

28

, 8086-8095. 

Meuth, S. G., Aller, M. I., Munsch, T., Schuhmacher, T., Seidenbecher, T., Meuth, P., 
Kleinschnitz, C., Pape, H. C., Wiendl, H., Wisden, W., & Budde, T. (2006). The 
contribution of TASK-1-containing channels to the function of dorsal lateral geniculate 
thalamocortical relay neurons. 

Molecular Pharmacology

Müller, Ch.M. (1992) Extra- and Intracellular Recording in the Slice, in: Kettenmann, H. 
& Grantyn, R. (eds.) Practical Electro-physiological Methods, Wiley-Liss, New York 

Ogden, D. (ed.) (1992) Microelectrode Techniques - The Plymouth Workshop Handbook, 
Second Edition, The Company of Biologists Ltd., Cambridge 

Schneider, N. L. & Stengl, M. (2006). Gap junctions between accessory medulla neurons 
appear to synchronize circadian clock cells of the cockroach Leucophaea maderae. 

Neurophysiol.

 

95

, 1996-2002. 

Seidenbecher, T. and H.C. Pape (2001) Contribution of intralaminar thalamic nuclei to 
spike-and-wave-discharges during spontaneous seizures in a genetic rat model of absence 
epilepsy, European Journal of Neuroscience, Vol. 13:1537-1546 

Windhorst, U. and H. Johansson (eds.) (1999) Modern Techniques in Neuroscience 
Research, Springer, Berlin, Heidelberg, New York 

Summary of Contents for EXT-16DX

Page 1: ...TEM DESCRIPTION FOR THE EXT 16DX EXTRACELLULAR AMPLIFIER VERSION 1 3 npi 2013 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com ...

Page 2: ..._________________________________________________________________ version 1 3 page 2 Table of Contents 1 Safety Regulations 3 2 EXT 16DX 4 2 1 System Description 4 2 2 Signal Flow Diagram 4 2 3 Description of the Front Panel 5 2 4 Description of the Rear Panel 7 2 5 Headstages 9 Headstage Assembly Elements 9 3 Literature 12 4 Technical Data 13 ...

Page 3: ...e is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an a...

Page 4: ...amplifier combined with fluorescence measurements The recorded signals are available at BNC connectors and an SCSI connector option for direct connection to a data acquisition board They are also linked to a SubD connector for connecting the optional R I T1DX for electrode resistance measurement and current injection The EXT 16DX can be operated manually by the controls at the front panel or remot...

Page 5: ...ch Switch to select recording either in referenced or in single ended mode In SINGLE ENDED mode recording with up to 16 electrodes with reference to ground is possible In REFERENCED mode the signal of the reference electrode is subtracted from the recording electrode resulting in reduction of noise The electrode acting as reference is selected by switch 11 The signal of the reference electrode can...

Page 6: ...ents In OFF position all displays and LEDs are switched off except the decimal point of display 4 that indicates that the amplifier is still powered on 8 LOWPASS SELECT switch Rotary switch for choosing the corner frequency for the lowpass filter The selected corner frequency is shown at display 6 9 GAIN SELECT switch Rotary switch for choosing the amplification factor gain for the extracellular s...

Page 7: ...taching a data acquisition board e g from National Instruments or Data Translation 2 IO R I T1DX connector SubD connector for connecting a R I T1M electrode resistance test current injection device 3 AUX connector optional Customized auxiliary connector 4 AUX connector optional Customized auxiliary connector 5 AUX connector optional Customized auxiliary connector 6 POWER SUPPLY connector Connector...

Page 8: ...ignal for the selected corner frequency of the highpass filter range 8V 7V 11 GAIN monitor connector BNC connector providing the monitor signal for the selected gain range 1V 10V 12 LOWPASS monitor connector BNC connector providing the monitor signal for the selected corner frequency of the lowpass filter range 8V 7V 13 OUT connectors BNC connectors providing the extracellular recorded signal The ...

Page 9: ...EXT MINI SE 16 DIL 3 shield 4 cable to R I T1DX green connector 5 cable to EXT 16DX blue connector Important The cables must be connected so that the white labels fit The recording headstage is attached to the EXT 16DX amplifier HEADSTAGE CON 1 connector blue The stimulation headstage is connected to the R I T1DX HEADSTAGE CON 2 connector green The recording headstage has also a connector for a Ne...

Page 10: ... must be connected so that the white labels fit Figure 8 shows a schematic of the top view of the headstage assembly with the connector for the test array or the NeuroNexus electrode respectively The test array is for testing purposes only It consists of resistors 1 M mimics the unit of the NeuroNexus electrode The two plugs 0 5 mm diameter next to the connector of the cable are labeled by a point...

Page 11: ... 9th pair next to the cable connector GND is left open since the electrode has no sites for grounding Note If the test array is plugged in with a wrong orientation nothing will be damaged but some electrodes are short circuited and others show 2 M instead of 1 M resistance when using the electrode resistance test with R I T1DX Figure 9 positioning of the NeuroNexus electrode on top of the headstag...

Page 12: ...1154 1165 o Lalley P M A K Moschovakis and U Windhorst 1999 Electrical Activity of Individual Neurons In Situ Extra and Intracellular Recording in U Windhorst and H Johansson eds Modern Techniques in Neuroscience Research Springer Berlin New York o Lambert F M Beck J C Baker R Straka H 2008 Semicircular canal size determines the developmental onset of angular vestibuloocular reflexes in larval Xen...

Page 13: ...n 6 dB octave corner frequencies Hz DC 0 1 0 3 0 5 1 3 5 10 30 50 100 300 500 800 1k 3k selected by rotary switch HIGHPASS Monitor 8V 7V LOWPASS Filter single pole attenuation 6 dB octave corner frequencies Hz 20 50 100 200 300 500 700 1k 1 3k 2k 3k 5k 8k 10k 13k 20k selected by rotary switch LOWPASS Monitor 8V 7V GAIN 10 20 50 100 200 500 1k 2k 5k 10k selected by rotary switch GAIN Monitor 1V 10V...

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