Manual: TIRF Microscope
Ee-1454
Martijn de Gruiter
Erasmus OIC
8
MDA: Time lapse imaging
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Activate the time-lapse option in MDA’s main window
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Set the desired interval and time
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Choose the desired illumination settings in the wavelength tab
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Or multiple when the option multiple wavelengths are selected
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Start recording by clicking on Acquire
Shut down system
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For live cell imaging:
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Remove Tokai Hit incubator from the stage and place it on the table
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Close the Tokai Hit incubator with a plastic well
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Clean objective carefully
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Remove oil with lens paper:
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Hold lens paper tight between your fingers and wipe over objective
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Clean lens with 2-propanol on your lens paper
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Cover stage with lens paper box
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Save data to OIC network storage (O:\ drive)
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To collect the data from your own pc, connect to network storage drive via the address:
user: guest, password: guest
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When the hard disk of the microscopes pc gets full, data will be deleted
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You are responsible for your own data, the networkdrive is only for transport
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If you are the last user of the day
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Shut down all devices
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Shut down mercury lamp as last
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Camera power is switched at the adapter box at the floor
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Switch off the power socket located at the floor
Contact
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Martijn de Gruiter
Be-343
tel. 31105
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Gert-Jan Kremers
Be-346
tel. 43578