Manual: TIRF Microscope
Ee-1454
Martijn de Gruiter
Erasmus OIC
6
Tab: Display:
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Auto scale option
o
Enable Auto scale or change custom auto scale
o
Full bit-range is saved to file, this is only for visualization
in the software
•
Visualize saturated pixels
Tab Acquire:
•
Be sure to set the Illumination choice to
“Current shutter”
Tab Special:
•
Gain options:
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10 MHz (EM gain), gain3 3x is standard setting
•
Control gain between 0 and 1000
•
Use "Frames To Avg" option to average images / or use
longer exposure time
Live image window
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Magnifying glass
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Display image in other LUT
o
Grayscale, Color by wavelength
•
Histogram of intensities
o
Manual scaling can be done with the orange arrows
•
Auto scaling options
o
Best fit range, 8/12/14/16 bit
o
Auto scale on/off
o
Scale within region
