Nikon eclipse e100 Instructions Manual Download Page 16 | Manualshive

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14

Microscopy (Detailed Procedure)

1)

Lamp Illumination

3)

Align the Diopter Ring with the Engraved Base Line

Turn on the power switch (turn to |) and the

lamp will come on. Turn the brightness control

dial to adjust the brightness of the viewfield.

(Turning the dial clockwise increases the

brightness; turning the dial counterclockwise

decreases it.)

Turn the diopter ring on the right eyepiece to

align its bottom edge with the engraved base

line. Turn and align the diopter ring on the left

eyepiece in the same way.

2)

Interpupillary Distance
Adjustment

Adjust the distance between the eyepieces to

merge the right and left viewfields into one.

(This is an adjustment to match the distance

between eyepieces with the distance between

your eyes).

Power ON

Darkens.

Brightens.

Merge the right and left
viewfields into one.

Match the bottom edge of the diopter ring
with the engraved base line.

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Summary of Contents for eclipse e100

Page 1: ...Microscope ECLIPSE E100 Instructions M413 E 06 10 NF 1 ...

Page 2: ......

Page 3: ...ut the prior consent of Nikon The instructions and specifications in this manual are subject to change without notice Although every effort has been made to ensure the accuracy of this manual if you find that any part of this manual is unclear or incorrect contact your nearest Nikon representative Some ECLIPSE E100 microscope sets may come with different components and accessories from those shown...

Page 4: ...s marked with this symbol may lead to injury or property damage Symbol Meaning Caution Hot surface This symbol label attached near the field lens unit the lamp is set underneath the field lens unit and on the bottom surface of the base plate the lamp is set above this place reminds you of the following WARNING Risk of burn injury The lamp and its surroundings including the field lens unit become h...

Page 5: ...oscope with the nameplate showing 100 110 120 V 250 V 1 A time lag low breaking type 5X20 miniature fuses two pieces For the microscope with the nameplate showing 220 230 240 V 250 V 0 5 A time lag low breaking type 5X20 miniature fuses two pieces 3 Check the Input Voltage The input voltage is indicated in two places at the rear of the microscope on the nameplate and above the AC IN socket Confirm...

Page 6: ...circuiting can also result when foreign matter is trapped inside the microscope If foreign matter or water has entered the microscope do not use the microscope and contact your nearest Nikon representative 7 Heat at the Light Source The lamp becomes hot during use Do not remove the field lens unit while the lamp is on and be sure the lamp has been off for 30 minutes before touching it When changin...

Page 7: ...the surrounding walls Install the microscope in the place that allows easy detachment of the power cord from the AC inlet in the event of emergency This microscope is a precision instrument Handle it gently Strong shocks and forcible operation will damage the instrument Shocks to the objectives especially could degrade image precision When carrying the microscope hold it at its arm with both hands...

Page 8: ...ve the clamps before use For details see p 27 Do not turn the knobs in opposite directions WARNING 13 Oil Immersion Observation Use only a minimum quantity of oil If too much oil is applied surplus oil could flow out to the stage and the condenser which could lead to degraded performance When using petroleum benzine or absolute alcohol to wipe off immersion oil or to clean the lenses follow the in...

Page 9: ...djusting the Torque of the Coarse Focus Knob 24 3 Photomicrography and TV Microscopy 24 4 Cord Hangers 24 VI Assembly 25 1 Assembly of Standard Set 26 2 Assembly of Additional Components 28 1 Condenser 28 2 Objectives 29 3 Specimen Holder 29 4 Eyepiece and Eyepiece Pointer 29 5 Monocular eyepiece tube 29 6 Other Accessories 29 3 Replacement of Consumable Materials 30 1 Replacing the Lamp 30 2 Repl...

Page 10: ...er should be positioned slightly lower than its upper limit Adjust the aperture diaphragm lever according to the objective Field lens unit Pull the field lens unit upward when changing lamp Lamp Halogen lamp of 6 V 20 W is used Fuse For the microscope with the nameplate showing 100 110 120 V 250 V 1 A time lag low breaking type 5X20 miniature fuses two pieces are used For the microscope with the n...

Page 11: ...9 1 Basic unit 2 Eyepieces 3 Eyepiece tube 4 Objectives 5 Condenser 6 Field lens unit 7 Lamp 8 Fuse x2 9 Power cord ...

Page 12: ...e located either to the right or the left of the stage Lateral stage motion X Axis knob ditto Fine focus knob Condenser clamp screw 10 Condenser vertical motion lever Use this lever to adjust the vertical position of the condenser p 19 This lever is located either on the right or the left side of the stage Coarse focus knob torque adjustment ring Used to adjust the tension torque of the coarse foc...

Page 13: ... 18 Voltage selector Fuse holder 19 Nameplate 12 Fine focus knob 9 Brightness control dial 10 Longitudinal stage motion Y Axis knob 11 Lateral stage motion X Axis knob 17 Fine focus knob 8 Power switch 4 Magnifying power indication ring 16 Coarse focus knob 15 Coarse focus knob torque adjustment ring 14 Condenser vertical motion lever 2 Revolving nosepiece ...

Page 14: ...adjust interpupillary distance Viewfields 2 Place specimen slide on the stage Coverglass up 3 Swing the 10x in the optical path Use this knob for focusing 1 Turn on the power switch 2 Widen or narrow to merge the viewfields into one 1 Match the bottom edge of the diopter ring with the engraved base line ...

Page 15: ...left eye 1 Switch to the 40x And focus with this ring Then focus with this ring 1 Check the magnifying power Repeat the steps 1 to 3 twice Repeat the steps 1 to 3 2 Move the lever to the magnifying power of the objective 3 Observe Turn off the power Wait till the microscope cools down before storing Focus with this knob ...

Page 16: ... it Turn the diopter ring on the right eyepiece to align its bottom edge with the engraved base line Turn and align the diopter ring on the left eyepiece in the same way 2 Interpupillary Distance Adjustment Adjust the distance between the eyepieces to merge the right and left viewfields into one This is an adjustment to match the distance between eyepieces with the distance between your eyes Power...

Page 17: ...age into focus by turning the coarse focus and then fine focus knob Direction of stage movement relative to focus knob rotation is shown in the figure There is no coarse focus knob on the side with the stage motion knobs There are both coarse and fine focus knobs on the opposite side of the stage motion knobs Do not turn the right and left focus knobs simultaneously in the opposite directions Do n...

Page 18: ...x or 4x objective Then change to a high power objective and rotate the fine focus knob for precise focusing then look into the eyepieces and lower the stage 1 When rotating the coarse focus knob while looking into the eyepieces be sure to turn it only in the direction that lowers the stage 2 When raising the stage using the coarse focus knob take your eyes off the eyepieces and look at the gap bet...

Page 19: ...lightly smaller than the working distance take your hands off the coarse focus knob The specimen is now almost in focus Look into the eyepieces and rotate the fine focus knob in the direction that lowers the stage Method 2 Swing the 40x objective into the optical path While looking at the microscope from the side rotate the coarse focus knob until the specimen almost touches the objective about 0 ...

Page 20: ...obs to bring the specimen in focus 2 Switch back to the 10x or 4x objective While looking into the right eyepiece with your right eye focus on the specimen by rotating the right diopter ring and not using the focus knob 3 While looking into the left eyepiece with your left eye focus on the specimen by rotating the left diopter ring and not using the focus knob 4 Repeat the steps 1 to 3 Focus with ...

Page 21: ... Objective Selection Rotate the revolving nosepiece to the desired objective magnification The objective will click into place when rotated into position Adjust the aperture diaphragm lever according to the selected objective see the procedure 9 9 Aperture Diaphragm Adjustment Set the condenser aperture diaphragm lever to the same figure as the magnification of the objective in the optical path Co...

Page 22: ...nation Do not use it to control brightness Use the brightness control dial to control the brightness The objective s numerical aperture is indicated on the side of the objective 40x 0 65 magnifying power 40x numerical aperture 0 65 To observe the diaphragm image remove the eyepiece and look down the open tube The eyepiece is fastened to the eyepiece tube Loosen the screw before removing the eyepie...

Page 23: ...he microscope Unplug the power cord Wait until the field lens unit is cool enough to touch Cover the microscope with the vinyl dust cover Before covering the microscope make sure that the field lens unit is cool enough to touch When carrying the microscope hold it at its arm and the bottom of its base ...

Page 24: ...front of the condenser from the long hole on the stage Bring the specimen back over the condenser and slowly raise the condenser Objective Rotate the revolving nosepiece to move the objective out of position Add a drop of oil to the specimen Slowly rotate the revolving nosepiece to bring the objective back into position Eliminate Air Bubbles Make sure that air bubbles are not trapped during oil ap...

Page 25: ...zine Cautions on Handling the Immersion Oil Close the container cap tightly after use Make sure that the cap is closed tight after refilling the container Check the cap periodically to make sure it has not come loose allowing oil to leak out Do not press the container hard Oil could splash out If you find an oil drips around the container wipe them off Avoid contact of immersion oil with eyes or s...

Page 26: ...s located at the back of the coarse focus knob To decrease the tension turn the ring clockwise Do not decrease the tension too much If it is too loose the stage will fall under its own weight Cord Hangers Push the cord hangers optional into the holes on the rear of the microscope The hangers can be used for winding the power cord around when the microscope is not in use To remove the hangers use a...

Page 27: ...e Be sure to follow the instructions written therein Also make sure that the power switch is off turned to before assembly to prevent electrical shock Tools Required for Assembly Hexagonal wrench one hexagonal wrench is provided with the microscope and the eyepiece tube flatblade precision screwdriver ...

Page 28: ... V The voltage can be set to AC 100 V 110 V or 120 V For the microscope with the nameplate showing 220 230 240 V The voltage can be set to AC 220 V 230 V or 240 V Changing the Voltage Setting 1 Turn off the power switch turn to and unplug the power cord 2 Remove the fuse holder using the flatblade precision screwdriver Use the tip of the screwdriver to push the two lock plates toward the center of...

Page 29: ...aining the vertical movement of the focusing mechanism The plate is fastened with 3 bolts Remove the bolts and the plate 3 Installing the Eyepiece Tube Loosen the eyepiece tube clamp screw by hand and place the eyepiece tube on the circular dovetail mount Tighten up the eyepiece tube clamp screw by hand Shipping clamps Eyepiece tube clamp screw CAUTION When detaching the eyepiece tube hold it firm...

Page 30: ...l path If the diffuser image is seen on the viewfield slighty raise or lower the condenser till the diffuser image is gone 28 2 Assembly of Additional Components 5 Connecting the Power Cord Turn off the power switch of the microscope turn to Connect one end socket of the supplied power cord to the AC IN socket on the rear of the microscope Connect the other end plug to an AC line receptacle with t...

Page 31: ...eces are attached to the eyepiece tube before shipment When removing an eyepiece loosen the eyepiece set screws using the hexagonal wrench provided and then remove the eyepiece When attaching an eyepiece push the eyepiece down to its full limit and tighten up the set screw Be careful not to tighten too hard When changing to a 15x eyepiece optional be sure to change both the right and left eyepiece...

Page 32: ...alling a new lamp If the contacts are damaged they may cause poor illumination or overheating Insert the lamp s contact pins fully into the socket holes If the pins are loose the lamp could come loose or result in a contact failure which will cause overheating or smoke Be sure to put the field lens unit back in place after replacing the lamp Never turn on the lamp without the field lens unit Do no...

Page 33: ... showing 220 230 240 V 250 V 0 5 A time lag low breaking type 5X20 miniature fuses two pieces 1 Turn off the power switch turn to and unplug the power cord 2 Wait about 30 minutes until the lamp and its surroundings are cool enough to touch 3 Hold the field lens unit at the dents on both sides and pull it upward slowly while slightly moving laterally to remove it 4 Remove the old lamp 5 Hold a new...

Page 34: ...he voltage provided in your region appears in the window of the fuse holder 5 Put the fuses and the fuse holder back in place Be sure to push both sides of the fuse holder firmly till it clicks into place Fuse holder Voltage selector Fuses Window Lock plate Voltage indication CAUTION Make sure that the contact of the fuse is not damaged before installing a new fuse If the contact is damaged a malf...

Page 35: ...index of the medium air immersion oil etc between the objectivelens and the specimen or condenser and α is half of the maximum angle at which light enters or leaves the lens from or to a focused object point on the optical axis The larger the numerical aperture the brighter the image and the higher the resolution 3 Resolving Power The ability of an optical system to discriminate between two discre...

Page 36: ...s of the specimen image in focus extending above and below the focused image plane The larger the N A of the objective the shallower the depth of focus Depth of focus μm Above is an approximation assuming the resolving power of an eye as 2 minutes λ is the wavelength of light used The depth of focus in the table on P 33 is indicated for λ 0 55μm n is the refractive index of a medium between the ob...

Page 37: ...y lower than the upper limit P 19 Condenser not installed correctly Install correctly P 28 Field lens unit not installed correctly Install correctly P 31 Lamp not installed correctly Install correctly P 31 Dirt or dust on the lens condenser objective field lens eyepiece specimen Clean the lens P 40 Optical path selection lever in the intermediate position on trinocular eyepiece tube Optical path s...

Page 38: ... not used for oil immersion observation Aperture diaphragm opened or closed too far Close or open properly P 19 20 Immersion oil found on dry type objective especially 40x and 60x objective Correction ring on the objective not adjusted only for the objective with the correction ring Adust the ring according to the cover glass thickness Dust or dirt on the eyepieces objective or condenser Clean the...

Page 39: ...not used Use blue filter P 27 Lamp voltage too low Adjust the voltage by rotating the brightness control dial P 14 Image too bright Lamp voltage too high Adjust the voltage by rotating the brightness control dial P 14 Insufficient brightness Lamp voltage too low Adjust the voltage by rotating the brightness control dial P 14 Incorrect input voltage Using the voltage selector select the voltage tha...

Page 40: ...point too large when switching from one objective to another Diopter not adjusted correctly Adjust P 18 Objectives being loose Screw onto the revolving nosepiece securely Note Do not switch the objective directly to 60x or 100x from 4x Always focus on the specimen with 10X objective before switching to high magnification objectives Specimen image jumps when specimen is moved by the stage Specimen ...

Page 41: ... specified lamp P 30 Lamp bulb not inserted Insert correctly P 30 Lamp bulb burnt out Replace bulb P 30 Flickering or unstable lamp brightness Lamp bulb about to fail Replace bulb P 30 Power cord not correctly connected Connect correctly P 28 Bulb not correctly inserted into socket Insert correctly P 30 Sudden lamp failure Incorrect lamp used Use the specified lamp P 30 Incorrect input voltage Sel...

Page 42: ...re Cleaning the Microscope Avoid using organic solvents such as alcohol ether thinner etc to clean the microscope Doing so could result in discoloration of the painted or plastic parts or in peeling of the printed characters We recommend that you use a silicon cloth to wipe down the painted or plastic parts of the microscope More persistent dirt may be cleaned using gauze lightly moistened with mi...

Page 43: ...Nosepiece 4 hole fixed type 6 Electrical Specifications Lamp Rating Halogen lamp 6V 20W PHILIPS 7388 or OSRAM HLX 64250 Output Rating 6V 3 3A max Input Rating Model for 100 110 and 120 V AC Areas Input voltage Select from 100 V 110 V or 120 V AC by relocating the fuse holder in the AC inlet Frequency 50 60 Hz Voltage fluctuation 10 Rated current 0 5 A Fuse rating 250 V 1A time lag low breaking typ...

Page 44: ...ductor grounding Type H05VV F 3 m long maximum rated at 250 V AC minimum Protection Class Class I 7 Operating Environment Room Temperature 0 to 40 C Relative Humidity 85 Max No condensation should form Altitude 2000m Max Pollution Degree 2 Installation Category Overvoltage Category Category 2 For indoor use only 8 Storage Environment Room Temperature 20 to 60 C Relative Humidity 90 Max No condensa...

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