Chapter 5 Troubleshooting
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Fluorescence image is dark (even though the light source is turned on).
Problems
Check Items
Reference
The diascopic illumination is not bright enough.
Adjust the illumination by turning the brightness control
knob close to the maximum position.
3.2.1
The room is too bright.
Dim the ambient light.
3.10.3
Weak contrast in fluorescence image
Problems
Check Items
Reference
The objective or cover glass is dirty.
Clean the lens.
3.3.1
The immersion oil is emitting fluorescence.
Use a low-fluorescence oil (Nikon immersion oil)
specified by Nikon.
3.4.2
The slide glass is emitting fluorescence.
Use a non-fluorescent glass slide.
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5.1.5 Problems in Dark-field Microscopy
Incomplete field of view or no field of view seen
Problems
Check Items
Reference
The dark-field slider is not at the click position (the
annular diaphragm is not in the optical path).
Move the slider until it clicks.
2.5
4.2.5
The objective's NA is not in this microscope's
supported range for dark-field microscopy.
Bring the dark-field objective into the optical path.
3.10.4
No dark-field image seen
Problems
Check Items
Reference
You are not using a dark-field slider.
Mount a phase contrast slider and bring the
appropriate position into the optical path.
2.5
4.2.5
The objective's NA is not in this microscope's
supported range for dark-field microscopy.
Bring the dark-field objective into the optical path.
3.10.4
