Chapter 5 Troubleshooting
111
Chapter 5
Troubleshooting
5
Troubleshooting
Misuse of this product may adversely affect performance, even if this product is properly functional. If any of
the following problems occurs, be sure to check the following table for possible causes before requesting
service.
If you detect problems that are not listed below or the problem still persists after measures are taken, unplug
the power cord of the device and contact your nearest Nikon representative.
5.1 Problems With the Microscope Image
5.1.1 Common Problems for Each Microscopy
Dirt or dust in the field of view
Problems
Check Items
Reference
The specimen is dirty if dirt or dust moves when the
specimen is moved on the stage.
Clean the specimen.
3.3.1
Objective is dirty if dirt or dust disappears when the
objective is switched.
Clean the objective.
6.1
The eyepiece is dirty if dirt or dust does not move
when the stage or objective is moved.
Clean the eyepiece.
6.1
Aperture diaphragm is closed too far.
Open properly. (
→
Section 3.5)
3.8
Poor image quality, low resolution, or contrast too low or too high
Problems
Check Items
Reference
No coverglass is attached to the slide.
Attach a coverglass 0.17 mm thick.
―
Coverglass is too thick or too thin.
Use a coverglass of the specified thickness (0.17 mm).
―
Slide is upside down.
Turn over the slide so that the cove glass faces up.
3.3.1
Lenses or specimen are dirty or dusty.
Check dirty or dusty parts, and then clean them as
appropriate by reference to the problems described in
“Dirt or dust in the field of view.”
―
Aperture diaphragm is opened or closed too far.
Close or open properly.
3.8
Field diaphragm is opened or closed too far.
Close or open properly.
3.9
No immersion oil is applied to the tip of the
oil-immersion objective.
Apply Nikon immersion oil to the objective.
3.4.2
Nikon immersion oil is not used for oil-immersion
observation.
Apply Nikon immersion oil to the objective.
3.4.2
Air bubbles in immersion oil.
Remove bubbles.
3.4.2
Immersion oil adhering to the tip of the dry type
objective.
Clean the lens.
6.1
No coverglass is attached to the slide for observation.
Attach a coverglass.
―
Image too bright
Problems
Check Items
Reference
Setting of the brightness control knob
Adjust the brightness by rotating the brightness control
knob.
3.2.1
