4
BE SAFE. ALWAYS MAKE SURE THAT THERE IS NO PRESSURE IN THE CELL BEFORE LOOSENING THE
BOLTS, AND THAT THE BOLTS ARE SECURELY FASTENED BEFORE APPLYING PRESSURE TO THE CELL.
USERS MUST ALWAYS WEAR PROTECTIVE APPAREL, INCLUDING EYEWEAR AND MUST BE TRAINED IN
WORKING WITH HIGH PRESSURE.
Operator’s Manual for the Pressure Injection Cell
desired pressure.
When the injection is finished, close the
gas tank valve. Then turn the knob on the
3-way valve so that it points toward the vent.
This depressurizes the injection cell. Now
you can loosen the nut over the ferrule and
remove the capillary, loosen the three bolts,
remove the top, and remove your sample
tube.
Use a wrench to tighten the nut, forcing the ferrule
to squeeze tightly around the capillary.
USE OF ADAPTER FOR 1 ML TUBES
We supply one adapter with the pressure
injection cell so that 1 ml tubes are as easy to
work with as taller tubes.
Photograph of a 1 ml tube with a hinged cap (left)
and of a 1 ml tube with the hinge cut off and the
bottom placed in an adapter composed of soft
tubing (right).
The central hole in the base of the pressure
injection cell is sized so that 1.5 or 2 ml
sample tubes will protrude above the surface,
making placement and retrieval easy. A
(surprisingly) simple adapter will enable 1 ml
microcentrifuge tubes to protrude, too. The
adapter is a piece of soft tubing (1/4 inch
inside diameter, 3/8 inch outside diameter).
As with any tube with a hinged cap, first cut
off the hinge so that the top of the tube will fit
in the recess in the top of the pressure
injection cell. Next, slide the adapter onto the
bottom of the 1 ml sample tube, as shown in
the figure below.
STIRPLATE OPERATION
(PC77-MAG model only)
Operation of the stirplate is straightforward.
The power supply should be plugged in to the
jack at the rear. The speed control knob is on
the left. Clockwise rotation increases the
speed. The power switch is on the right.
MAINTENANCE
Periodically, the Teflon ferrule should be
replaced. Simply remove the nut covering the
ferrule, remove the ferrule, place a new
ferrule, tapered side down into the fitting, and
replace the nut. Your pressure injection cell
comes with 10 ferrules (plus one installed in
the top). Additional ferrules can be purchased
directly from Chromatography Research
Supplies (part number 214204, 1/8” to 0.4mm
PTFE ferrules), at www.chromres.com
DISCONNECTING AND
RECONNECTING THE UNIT
Before you disconnect the pressure injection
cell, mark lines on the nut and the valve,
close the valves by the pressure source, and
release any pressure inside the cell by
opening the 3-way valve. When you recon-
nect the unit, you can use the same ferrule
and tubing. Simply tighten the nut slightly
more than last time. You should feel the
wrench get very hard to turn once you go
beyond the nut’s previous position.
