background image

 

 

c. 

Rotate coarse focusing controls until specimen comes into focus. 

 

d.  Adjust fine focus controls until specimen is in sharp focus. 

 

e.  Adjust diopter for possible difference in eyesight between user’s left and right eyes.  If user has 20/20 vision, this 

adjustment should not be necessary. 
 
1.  Using right eye, peer into the right eyepiece tube.  Adjust sharpness of image by utilizing fine focus controls, 

without touching the diopter ring. 

 

2.  Using left eye, peer into the left eyepiece tube.  Adjust sharpness of image by turning diopter adjustment located 

on left eyepiece tube. 

 

f. 

Adjusting the aperture (opening) of iris diaphragm. 

 

Iris diaphragm should not be used to control the brightness of illumination, 

use the illuminator light intensity 

control to adjust light level. Iris diaphragms are designed to help achieve high resolution of specimen and provide 
contrast in the image.  Smaller apertures will deliver higher contrast to image.  However, closing aperture too much will 
reduce resolution.  Experimentation is the best method of determining the correct opening of diaphragm.  Some 
suggested openings for iris diaphragm are: 

 

OBJECTIVE

   

 

DIAPHRAGM OPENING

 

4x 

 

 

From fully closed to 1/8 open 

10x 

 

 

1/8 to 1/4 open 

40x 

 

 

1/4 to 1/2 open 

100x 

 

 

1/2 to 3/4 open 

 

g.  Usage of special built-in neutral filter. 

 
1.  Placing a filter into the optical path will absorb some of the light from the illuminator base, limiting the light output.   

 

2.  When using 4x and 10x objectives the special neutral filter must be in optical path. 

 

3.  When using the higher power 40x and 100x objectives remove filter from optical path. 

 

4.  To remove filter, grasp filter holder knob and swing out filter holder. 

 

h.  Changing magnification. 

 
1.  Rotate revolving nosepiece to position 10x objective into optical path. 

 

2.  This microscope has been parfocalled, which allows changes from one objective to another while requiring only a 

slight adjustment of the fine focus controls. 

 

3.  When changing to the 40x and 100x objective lens, care must be exercised when positioning these lenses into the 

optical path, in order to prevent damaging the front lens element and specimen slide. 

 

4.  In order to obtain maximum resolution of the 100x oil immersion lens, it is necessary to apply immersion oil 

between the cover glass of slide and front lens of the objective. 

 

a)  Use of a very small amount of immersion oil is required. 

 

b)  All air bubbles must be removed from between lens and slide by gently rotating nosepiece back and forth. 

 

c)  When finished viewing, all parts that come in contact with oil must be cleaned.  Failure to do so could 

permanently damage the 100x oil immersion objective lens.  Use Windex to clean immersion oil off lens 
surfaces is recommended. 

 

Objective Specification Chart

 

Objective 

N.A. 

Color Code 

Ring 

Field of 

View 

Working 

Distance 

Magnification with 

WF10X eyepiece 

Din 4X 

0.10 

Red 

4.5mm 

26.4mm 

40X 

Din 10X 

0.25 

Yellow 

1.8mm 

5.5mm 

100X 

Din 40X  

0.65 

Blue 

0.45mm 

0.48mm 

400X 

Din 100X 

1.25 

White 

0.18mm 

0.06mm 

1000X 

 

Summary of Contents for 158-LED

Page 1: ...entific Instruments Inc 11113 Landmark 35 Drive San Antonio Texas 78233 Phone 210 590 9010 Fax 210 590 1104 INSTRUCTIONS FOR MODEL 158 LED COMPOUND MICROSCOPES Copyright 11 09 04 National Optical Scientific Instrument Inc ...

Page 2: ...erpupillary adjustment grips located on both left and right side of diopter scale Mark on side of eyepiece tube for indexing diopter reading Head locking screw Arm of microscope stand Knobs controlling X and Y movement of mechanical stage Coarse focus knob Fine focus knob Power on off switch Tension adjustment collar behind focus knob on left side Rack stop adjustment screw Knurled locking screws ...

Page 3: ...son repack in the styrofoam container and then pack the styrofoam in other corrugated shipping container for optimum protection Use of the styrofoam alone will not provide adequate protection in transit and will void your warranty DESCRIPTION OF COMPONENTS 1 EYEPIECE ocular lens Lens closest to the eye magnifies the primary image formed by the objective lens One eyepiece is equipped with a pointer...

Page 4: ... into DC recharging socket located on back of microscope base Your automatic switching recharger operates on 100 to 240 volts AC 50 60 Hz Plug charger into your AC wall outlet Battery recharger is equipped with an automatic trickle charge feature the red LED indicator lamp located on recharger will be illuminated when batteries are receiving maximum charge After batteries are charged the red LED i...

Page 5: ...base limiting the light output 2 When using 4x and 10x objectives the special neutral filter must be in optical path 3 When using the higher power 40x and 100x objectives remove filter from optical path 4 To remove filter grasp filter holder knob and swing out filter holder h Changing magnification 1 Rotate revolving nosepiece to position 10x objective into optical path 2 This microscope has been ...

Page 6: ... rack stop socket set screw With fine focus adjustment at mid range focus on standard slide until sharp image is obtained Rotate rack stop set screw in clockwise direction until tight tighten locking nut b Coarse focus tension adjustment prevents the stage from drifting down from its own weight and causing the image to move out of focus This has been adjusted at the factory but over the course of ...

Page 7: ...ours so you don t have to do this exercise very often To open the illuminator field lens housing use hex wrench supplied with your microscope to loosen hex screw on side of lens housing Remove lens housing to expose LED bulb Remove bulb by grasping the plastic base of bulb and gently pulling straight up Insert new LED bulb replace lens housing and tighten hex screw to secure lens housing in place ...

Page 8: ... service for 1 year from date of purchase It does not cover damage resulting from abuse or misuse repairs or alterations performed by other than the manufacturer or damage occurring in transit Software 90 day limited warranty Manufacturer warrants software to be free from defects in material and workmanship under normal use and service for 90 days from date of purchase Other than set forth above N...

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