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INTRODUCTION

 

 
Thank you for your purchase of a National microscope.  It is a precision instrument carefully checked to assure that it reaches 
you in good condition.  It is designed for ease of operation and years of carefree use.  The information in this manual probably 
far exceeds what you will need to know in order to operate and maintain your microscope.   However, is provided to answer 
questions that might arise, and to help you avoid any maintenance expense that may be unnecessary. 
 
Your new compound microscope is a high performance microscope with high quality achromatic objective lenses that provide 
good resolution and optical centering.  The microscope is designed with a built-in mechanical stage providing a travel range of 
75mm x 30mm in the X and Y direction with graduation reading up to 0.1mm for accurate positioning of specimen.  Also 
included is a ball bearing quadruple nosepiece, separate coarse and fine focusing, spiral mounted N.A. 1.25 Abbe condenser, 
and built-in heat free LED illumination equal to a 20 watt bulb. 
 
Carefully read these instructions before operating microscope.  They will permit you to use your new microscope to its fullest 
capability.  Referring to diagram on page 2 identifies nomenclature used to describe components and controls. 
 

UNPACKING 

 
1.  The microscope and accessories have been carefully packed to assure that they reach you in the best possible condition.  

Check package containers to make sure all components are accounted for.   

 

a.  Microscope stand with the following components already installed WF10x eyepieces (pair), 4 objectives, condenser, 

and three rechargeable batteries. 

 
b.  Two rubber eyeshields, specimen holder, recharger, dust cover, 0.9mm “L” type key wrench (for lamp replacement), 

and 2mm “L” type key wrench (for rack stop adjustment), and instruction manual. 

 
2.  Save packing container in case it becomes necessary to ship the microscope for any reason, repack in the styrofoam 

container, and then pack the styrofoam in other corrugated shipping container for optimum protection.  Use of the 
styrofoam alone will not provide adequate protection in transit, and will void your warranty. 
 

DESCRIPTION OF COMPONENTS 

 
1.  EYEPIECE  (ocular lens):  Lens closest to the eye, magnifies the primary image formed by the objective lens. One 

eyepiece is equipped with a “ pointer” that rotates as the eyepiece is turned. 

  
2.  OBJECTIVE TURRET (nosepiece):  Revolving turret which holds objective lenses, permits changes of magnification by 

rotating different powered objective lenses into optical path.  Reverse position permits easier access to stage when 
positioning specimen slides. 

 
3.  OBJECTIVE LENS:  Lens closest to the object being viewed, forms first magnified image of the specimen. 
 
4.  MECHANICAL STAGE:  Permits precise, mechanical manipulation of the specimen slide. 
 
5.  SPECIMEN HOLDER: Holds specimen slide. 
 
6.  STAGE:  Platform of the microscope where the specimen slide is placed. 
 
7.  CONDENSER:  A focusable 1.25 N.A. Abbe condenser lens positioned under center of stage condenses light rays from 

substage illumination and fills the back lens element of objective lens to improve image resolution. 

 
8.  IRIS DIAPHRAGM:  Attached to bottom of Abbe condenser, controls aperture of light by moving control lever left or right. 
 
9.  FILTER HOLDER:  Attached to bottom of iris diaphragm with swing out built in neutral filter.  Swing out filter should be 

removed from optical path when using 40x or 100x objective lens. 

 
10.  SAFETY RACK STOP:  When properly adjusted, controls maximum upward travel of stage.  Prevents higher power 

objectives from breaking specimen slides, prevents damage to objective lenses.  This stop has been pre-adjusted at the 
factory. 

 
11.  FOCUSING KNOBS:  Coarse focusing knobs (larger knobs) located on each side of arm, raise or lower stage to bring 

specimen image into focus.  Fine focus knobs (smaller knobs located just below coarse focusing knobs) permit more 
precise image adjustment. 

 
12.  ILLUMINATION:  Built-in substage LED illuminator provides constant, reliable pre-focused illumination. 
 

Summary of Contents for 158-LED

Page 1: ...entific Instruments Inc 11113 Landmark 35 Drive San Antonio Texas 78233 Phone 210 590 9010 Fax 210 590 1104 INSTRUCTIONS FOR MODEL 158 LED COMPOUND MICROSCOPES Copyright 11 09 04 National Optical Scientific Instrument Inc ...

Page 2: ...erpupillary adjustment grips located on both left and right side of diopter scale Mark on side of eyepiece tube for indexing diopter reading Head locking screw Arm of microscope stand Knobs controlling X and Y movement of mechanical stage Coarse focus knob Fine focus knob Power on off switch Tension adjustment collar behind focus knob on left side Rack stop adjustment screw Knurled locking screws ...

Page 3: ...son repack in the styrofoam container and then pack the styrofoam in other corrugated shipping container for optimum protection Use of the styrofoam alone will not provide adequate protection in transit and will void your warranty DESCRIPTION OF COMPONENTS 1 EYEPIECE ocular lens Lens closest to the eye magnifies the primary image formed by the objective lens One eyepiece is equipped with a pointer...

Page 4: ... into DC recharging socket located on back of microscope base Your automatic switching recharger operates on 100 to 240 volts AC 50 60 Hz Plug charger into your AC wall outlet Battery recharger is equipped with an automatic trickle charge feature the red LED indicator lamp located on recharger will be illuminated when batteries are receiving maximum charge After batteries are charged the red LED i...

Page 5: ...base limiting the light output 2 When using 4x and 10x objectives the special neutral filter must be in optical path 3 When using the higher power 40x and 100x objectives remove filter from optical path 4 To remove filter grasp filter holder knob and swing out filter holder h Changing magnification 1 Rotate revolving nosepiece to position 10x objective into optical path 2 This microscope has been ...

Page 6: ... rack stop socket set screw With fine focus adjustment at mid range focus on standard slide until sharp image is obtained Rotate rack stop set screw in clockwise direction until tight tighten locking nut b Coarse focus tension adjustment prevents the stage from drifting down from its own weight and causing the image to move out of focus This has been adjusted at the factory but over the course of ...

Page 7: ...ours so you don t have to do this exercise very often To open the illuminator field lens housing use hex wrench supplied with your microscope to loosen hex screw on side of lens housing Remove lens housing to expose LED bulb Remove bulb by grasping the plastic base of bulb and gently pulling straight up Insert new LED bulb replace lens housing and tighten hex screw to secure lens housing in place ...

Page 8: ... service for 1 year from date of purchase It does not cover damage resulting from abuse or misuse repairs or alterations performed by other than the manufacturer or damage occurring in transit Software 90 day limited warranty Manufacturer warrants software to be free from defects in material and workmanship under normal use and service for 90 days from date of purchase Other than set forth above N...

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