background image

Objective Specification Chart

 

 

Objective 

N.A. 

Color Code 

Ring 

Field of 

View 

Working 

Distance 

Magnification with 

WF10X eyepiece 

Din 4X 

0.10 

Red 

4.5mm 

18.5mm 

40X 

Din 10X 

0.25 

Yellow 

1.8mm 

6.4mm 

100X 

Din 40X retractable 

0.65 

Blue 

0.45mm 

0.5mm 

400X 

 
 

c.  Also note that each objective has a color ring, which permits you to instruct changes in magnification by referring 

to an easily observed color rather than to a number. 

 

d.  The microscope has been parfocalled at the factory, which allows easy change from one magnification to another, 

requiring little or no adjustment of the fine focus knobs. 

 
e.  As magnification is increased, the field of view (area of specimen seen through the microscope) will decrease.  

That is why it is easier to find the specific area of interest on the specimen by starting with the lowest 4x objective 
lens, before increasing magnification with the 10x or 40x objective lens. 

 
f. 

NOTE:  Care must be taken when rotating the 40x objective into place.  This lens has a spring retractable 
mechanism which retracts slightly into its housing if the front of the lens strikes the specimen slide.  With fine 
focus adjustment at mid-range, the rack stop has been adjusted at the factory to assure the 40x lens will clear the 
thickness of a normal specimen slide and cover slip.  However, if the rack stop has been improperly adjusted, or if 
using a thicker than normal slide or cover slip, moving the 40x lens too quickly or carelessly could cause damage 
to the front lens element or to the slide. 

 
g.  Do not let the front lens element come into contact with a wet slide surface, as prolonged contact with any 

moisture could damage the lens.  If lens is exposed to moisture, promptly wipe with soft tissue to remove 
moisture. 

 
 

MAINTENANCE 

 

WARNING:  For your own safety, turn switch to OFF position.  Remove adapter from the power source and 
detach plug from jack located at back of microscope base before maintaining microscope. 

 
1.  OPTICAL MAINTENANCE 
 

Do not attempt to disassemble any lens components.  Consult a microscope service technician when any repairs not 
covered by instructions are needed. 
 
Prior to cleaning any lens surface, brush dust or dirt off lens surfaces using a camel hair brush.  Or use air to blow 
dust and lint off surfaces.  Use of compressed air in a can,  available at any computer supply store, is a good source 
of clean air. 
 
Do not remove eyepieces or objective lenses to clean.  Clean only the outer lens surface.  Breath on lens to dampen 
surface, then wipe with lens paper or tissue or use a cotton swab moistened with distilled water.  Wipe lenses with a 
circular motion, applying as little pressure as possible.  Avoid wiping dry lens surface as lenses are scratched easily.  
If excessive dirt or grease gets on lens surfaces, a small amount of Windex can be used on a cotton swab or lens 
tissue.  To clean objective lenses, do not remove objectives from microscope.  Clean front lens element only, 
following same procedure. 
 
NOTE:  Fingerprints or other matter on the front lens element of the objective lens is the single most common reason 
that you will have difficulty in focusing the microscope.  Before having costly servicing done, or before returning to 
National for “warranty repair”, make certain to examine the front lens element with a magnifying glass or eye loupe for 
the presence of such contaminants.  If a microscope is returned to National for warranty repair, and it is determined 
that such contaminants are the problem, this is not covered under warranty and National will submit a cost estimate 
for cleaning. 
 
 
 

2.  MECHANICAL MAINTENANCE 

Summary of Contents for 131-RLED

Page 1: ...cientific Instruments Inc 6508 Tri County Parkway Schertz Texas 78154 Phone 210 590 9010 Fax 210 590 1104 INSTRUCTIONS FOR MODEL 131 RLED COMPOUND MICROSCOPES Copyright 3 31 10 National Optical Scientific Instrument Inc ...

Page 2: ... stage clips and permits precise mechanical manipulation of the specimen slide 5 STAGE Platform of the microscope where the specimen slide is placed 6 CONDENSER LENS A specially designed condenser lens fixed in center of stage condenses light rays from substage illumination and fills the back lens element of objective lens to improve image resolution 7 DISC DIAPHRAGM Rotating disc located below st...

Page 3: ...ity knob until image is illuminated and proceed as follows c In case of equipment malfunction see Troubleshooting procedures located at the back of this manual 3 Rotate coarse focus knobs to move stage down away from objective lens as far as possible 4 Place specimen slide cover slip facing up on stage with specimen centered over condenser lens in middle of stage 5 Rotate disc diaphragm until larg...

Page 4: ...promptly wipe with soft tissue to remove moisture MAINTENANCE WARNING For your own safety turn switch to OFF position Remove adapter from the power source and detach plug from jack located at back of microscope base before maintaining microscope 1 OPTICAL MAINTENANCE Do not attempt to disassemble any lens components Consult a microscope service technician when any repairs not covered by instructio...

Page 5: ...m battery charger into DC recharging socket located on back of microscope base Your automatic switching recharger operates on 100 to 240 volts AC 50 60 Hz plug recharger into your AC wall outlet The red LED indicator lamp located on recharger will be illuminated when batteries are receiving maximum charge After batteries are charged the red LED indicator lamp will turn to green and charger automat...

Page 6: ...wn Place slide on stage with cover slip up Slide cover slip too thick Use 0 17mm thick cover slip No 1 cover slip Poor resolution image not sharp Objective lenses dirty Clean objective lenses Eyepiece lens dirty Clean eyepiece lenses Too much light Adjust iris diaphragm Spots in field of view Eyepiece or condenser lens dirty Clean lens Specimen slide dirty Clean slide Spots in field of view can al...

Reviews: