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2.  Assure that light is available for illuminating the specimen.

 

 

a.  Your microscope has special LED illumination that is powered by 3 rechargeable AA nickel metal hydride 

batteries (supplied).  These batteries may be recharged, as required, using the recharger (supplied).  Each set of 
batteries may be recharged approximately 500 times before replacing, and each charge will provide up to 50 
hours of microscope operation.  The LED component (bulb) will last for up to 50,000 hours before replacement is 
required. 

 

WARNING 

 

DO NOT USE regular AA alkaline batteries.  Use of other than rechargeable AA nickel metal hydride 
batteries could result in batteries exploding during recharge.  ONLY USE THE SUPPLIED SWITCHING 
BATTERY RECHARGER WITH AUTOMATIC “TRICKLE CHARGE”. 

 

b.  It is recommended that you charge the batteries before initial use and after prolonged storage as the batteries 

may have discharged.  Plug output cord from battery charger into DC recharging socket located on back of 
microscope base.  Your automatic switching recharger operates on 100 to 240 volts AC 50/60 Hz. Plug recharger 
into your AC wall outlet.  Battery recharger is also equipped with an automatic “trickle charge” feature, the red 
LED indicator lamp located on recharger will be illuminated when batteries are receiving maximum charge.   After 
batteries are charged, the red LED indicator lamp will turn to green and charger automatically switches to “trickle 
charge”.   The charger can be left plugged in, but for safety reasons it is a good idea to disconnect the charger 
from the AC wall outlet and the output cord from recharging socket after 12 hours.  Batteries and charger may feel 
warm when charging, and unplugging the recharger is a safety precaution. 

 

Note that your microscope can be used during recharging.  

Simply turn “on/off” rheostat switch located on 

microscope base to “on” position, rotate intensity knob until image is illuminated and proceed as follows. 

 

c.  In case of equipment malfunction, see Troubleshooting procedures located at the back of this manual. 

 
3.  Rotate coarse focus knobs to move stage down (away) from objective lens as far as possible. 
 
4.  Place specimen slide, cover slip facing up, on stage with specimen centered over condenser lens in middle of stage. 
 
5.  Rotate disc diaphragm until largest aperture is positioned beneath condenser in center of stage. 
 
6.  Turn the objective turret until the 4x (smallest) objective lens “clicks” into position in the optical path.  Note that each 

time you change from one objective lens to another you should turn the turret until you hear the “click”, which 
indicates that the lens is properly indexed in the optical path. 

 
7.  While looking through the eyepiece, rotate coarse focusing knobs until specimen comes into focus. If image does not 

appear in field of view, move specimen slide slightly until image appears in field of view. 

 
8.  Adjust fine focus controls until specimen is in sharp focus. 
 
9.  Turn the disc diaphragm, observing that different apertures affect the sharpness of the image.  Turn diaphragm until 

sharpest possible image is obtained.  Note that when turning the disc diaphragm, you should hear a “click” as each 
aperture comes into proper position under the condenser lens.  If aperture is not properly positioned, you will observe 
shadows in the field of view when looking through the microscope. 

 
10.  Changing magnification 
 

a.  Magnification is changed by rotating objective turret until different objective lens is moved into optical path.  

Always turn turret until you hear the “click”, indicating that lens is properly indexed.  Otherwise, you will not be 
able to see anything when looking through the microscope. 

 
b.  Standard lenses provided with your microscope are a widefield 10x eyepiece. 4x. 10x, and 40x objectives.  The 

40x objective lens has a special spring retractable mechanism which retracts slightly if front of lens comes in 
contact with the specimen slide. See chart below for specifications on objectives. 

 

 

Summary of Contents for 131-RLED

Page 1: ...cientific Instruments Inc 6508 Tri County Parkway Schertz Texas 78154 Phone 210 590 9010 Fax 210 590 1104 INSTRUCTIONS FOR MODEL 131 RLED COMPOUND MICROSCOPES Copyright 3 31 10 National Optical Scientific Instrument Inc ...

Page 2: ... stage clips and permits precise mechanical manipulation of the specimen slide 5 STAGE Platform of the microscope where the specimen slide is placed 6 CONDENSER LENS A specially designed condenser lens fixed in center of stage condenses light rays from substage illumination and fills the back lens element of objective lens to improve image resolution 7 DISC DIAPHRAGM Rotating disc located below st...

Page 3: ...ity knob until image is illuminated and proceed as follows c In case of equipment malfunction see Troubleshooting procedures located at the back of this manual 3 Rotate coarse focus knobs to move stage down away from objective lens as far as possible 4 Place specimen slide cover slip facing up on stage with specimen centered over condenser lens in middle of stage 5 Rotate disc diaphragm until larg...

Page 4: ...promptly wipe with soft tissue to remove moisture MAINTENANCE WARNING For your own safety turn switch to OFF position Remove adapter from the power source and detach plug from jack located at back of microscope base before maintaining microscope 1 OPTICAL MAINTENANCE Do not attempt to disassemble any lens components Consult a microscope service technician when any repairs not covered by instructio...

Page 5: ...m battery charger into DC recharging socket located on back of microscope base Your automatic switching recharger operates on 100 to 240 volts AC 50 60 Hz plug recharger into your AC wall outlet The red LED indicator lamp located on recharger will be illuminated when batteries are receiving maximum charge After batteries are charged the red LED indicator lamp will turn to green and charger automat...

Page 6: ...wn Place slide on stage with cover slip up Slide cover slip too thick Use 0 17mm thick cover slip No 1 cover slip Poor resolution image not sharp Objective lenses dirty Clean objective lenses Eyepiece lens dirty Clean eyepiece lenses Too much light Adjust iris diaphragm Spots in field of view Eyepiece or condenser lens dirty Clean lens Specimen slide dirty Clean slide Spots in field of view can al...

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