EVE
TM
, Automatic Cell Counter ©2013 NanoEnTek Inc.
11
Operation
Cell mode
parameters
2.
The cell mode parameters are described below:
∙ Sensitivity
(refers to the contrast of the objects from the background).
Adjusting the sensitivity higher makes instrument more sensitive to
objects; useful for cells that do not stain well with trypan blue while
adjusting the sensitivity lower makes the instrument less sensitive and is
useful if there is a lot of background.
∙ Minimum cell size
is used to determine the low range of cell size to
include in the measurement. The algorithm first identifies all objects, and
calculates the average size (e.g., 15 µm). From the percent of average
size setting, the algorithm calculates the smallest object size to include in
the final measurement (e.g., 70% of 15 is 10.5 µm; 15–10.5 = 4.5 µm; 4.5
µm would be the smallest particle included in the count). Adjusting the
number up, increases inclusiveness thereby decreasing the lower cell
size range (e.g., 50% of 15 is 7.5 µm; 15–7.5 = 7.5 µm).
∙ Maximum cell size
is used to determine the high range of cell size to
include in the measurement. The algorithm first identifies all objects, then
calculates the average size (e.g., 15 µm). From the percent of average
size setting, the algorithm calculates the largest object size to include in
the final measurement (e.g., 200%; 200% of 15 µm = 30 µm; 30 µm is
the largest cell size included in the measurement.
∙ Circularity
is used to determine the objects to include in the
measurement based on roundness. Increasing the value from 80%
requires objects to be more round for inclusion in the measurement.
Decreasing the value from 80% allows objects to be less round. Adjusting
this may be useful if the cell type is not particularly circular or perhaps
oddly shaped.
After modifying any parameters,
press
Apply button
to make the changes.
To restore default parameters, press
Default button
.
3.
Press
Save protocol button
to create a new protocol.
