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ADAM - rWBC ©2013 NanoEnTek Inc.
13
Precision stage calibration
Each time the ADAM-rWBC is powered on, calibration of the precision
stage is performed using the Standard beads provided in the ADAM-
rWBC Kit. Calibration is successful if the test result is within range of
the value on the vial.
Refer to the ADAM-rWBC Kit Package Insert for specific instructions.
• The ADAM-rWBC must be calibrated after each start up and at least
once each day of use.
1. Let beads equilibrate to room temperature before use (~10 minutes).
2. Mix by shaking gently, including upside down.
3. Load 100 µL of the beads into the r-Slide. Let settle for 40-60 seconds.
4. Insert bead loaded r-Slide into ADAM-rWBC main device and press
‘ ‘ to start.
For Leukocyte Reduced samples
Blood samples should be tested within
48 hours after leukocyte
reduction.
The blood sample is mixed, and an aliquot removed and diluted with
r-Solution for staining. After staining, a sample is loaded into the r-Slide
and left to settle. After settling, the r-Slide is inserted arrow end first,
right side up, into the ADAM-rWBC and counted. Refer to the ADAM-
rWBC kit Package Insert for specific instructions.
CAUTION:
• Avoid bubbles as they may negatively affect results.
• Do not re-use r-Slides. Re-use of r-Slides may result in
errors in the result value.
• Do not use r-Solution, r-Slides or Standard beads after
their expiration date.
• Store r-Solution at 2~8 °C when not in use. The expiration data is
one year. Do not use after the expiration date shown on the
label. Open r-Solution may be stored at 2~8°C and used for up
to 6 months, but not past the expiration date.
Warning:
The instrument will not detect incorrectly diluted samples.
Also refer to ADAM-rWBC Kit Package Insert.
General operation
Preparing the
test sample