ML41biological microscope user manual
16
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When using the objectives with magnification higher than 20x, the
front lens of the condenser shall be shifted in the light path.
(3) Shift the 20x objective into the light path and focus on the
specimen.
(4) Rotate the field diaphragm adjusting ring
③
narrow the field
diaphragm to the minimum position. At this time, the image formed
by the field diaphragm can be observed in the eyepiece.
(5) Adjust the condenser lifting knob until the image is clear.
(6) Adjust the adjusting screw
④
until the image is in thee middle of
the field.
(7) Gradually widen the field diaphragm. If the image formed by the
field diaphragm is always in the middle of the field and can be
inscribed with the field, the condenser has been correctly aligned.
(Picture 17)
(8)When actually using the microscope, please slightly widen the field
diaphragm until the image circumscribes to the field.
3.9 Field diaphragm
The field aperture limits the diameter of the light beam entering the
condenser, so as to block the excess light at the periphery and enhance
the image response. When the imaging of the field aperture is just at
the outer edge of the field of view, the objective lens can play the
most superior performance and get the clearest specimen image.
3.10 Aperture diaphragm
The aperture aperture determines the numerical aperture of the
lighting system. Matching the numerical aperture of the lighting
system with that of the objective lens can result in better image
resolution, contrast and the increase the depth of field.
The contrast of the samples is usually low, thus, for the best
contrast and the clearest image, it is recommended to adjust the
aperture of the condenser to 70% ~ 80% of the numerical aperture of
the objective lens (Picture 18). The adjustment steps: remove the
eyepiece, observe through the eyepiece barrel, adjust the aperture
diaphragm adjusting ring
①
in Picture 19 until the image shown in
Picture18
Picture 17
Aperture
objective
Picture 19
