─
8
─
dsDNA detection.
2.1 dsDNA detection
Click the dsDNA icon to enter the following interface (Figure 5)
Figure 5 Sample detection
In Figure 5:
Standard curve: Select the established standard curve, and the curve information will be
displayed in the lower left corner.
Sample detection: After entering, the fluorescence value of the fluorescent sample can be
detected, and the sample concentration can be calculated according to the selected standard
curve.
Note:
a. When the 0.5mL sample tube is used for detection by the fluorometer, the volume of
the sample to be detected is 200uL. When the sample volume added during the detection is
less than 200uL, it needs to be supplemented to 200uL by the diluent. The concentration of
the sample detected at this time is the concentration of the sample in the tube, while The
instrument calculates the original sample concentration (that is, the actual concentration of the
added test sample) from the added sample volume and the diluent volume.
Actual sample volume
added
Standard
curve
selection