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5. PHOTOMICROGRAPHIC PROCEDURE
To ensure vibration free operation, set the microscope on a sturdy vibration free table or a bench
with a vibration proof device.
For the same total magnification, select a combination of the highest possible objective
Magnification and lowest possible projection lens magnification to achieve the utmost image
definition and contrast.
To ensure optimal illumination, check the position and centring of the lamp andposition of the
condenser.
Select a blue filter for routine application.
Adjusting the field diaphragm is important for the purpose of limiting extraneous light that may
cause flare and lower the contrast. Stop down the diaphragm to achieve an illuminated area slightly
larger than that of the field of view.
A change of depth of focus, contrast and resolution of image is attainable with anaperture setting
that is 2/3 of the objective N.A.
This is high precision production; infelicity handling and operation will reduce or damage the
function of production.
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