OPERATION OF THE BIOLOGICAL MICROSCOPE
The SILVER150 Series biological microscope with adjustable condenser can do as follow:
1. Raise the stage by turning the coarse focus knob.
2.
Completely lower the condenser carrier by turning the condenser focus knob.
3.
Turn the condenser focus knob to raise the condenser up to the workingposition.
USING YOUR MOTIC MICROSCOPE
1. Use the stage clips or slide holder mechanism to secure the slide in place. Be sure the
specimen is centered over the opening in the stage.
2. Rotate the nosepiece to place the lowest power (4X) objective over the specimen. Be sure
the objective “clicks” into position.
3. Turn on the illumination by pressing the on/off switch
4. Adjust the Siedentopf binocular head (by moving the eyepiece tubes up and down in an
arc-like motion, similar to adjusting binoculars) until one perfect circle is seen in the field of
view.
5. Open the iris diaphragm to its largest aperture.
6. While viewing through the eyepiece(s), rotate the coarse focus knob slowly and carefully
to bring the specimen into focus. The specimen may require some centering in the field of
view at this time. By using the fine focusing knob, slowly and carefully refine the focus to
clearly observe the fine details of the specimen.
7. If the image of the specimen appears pale, the aperture of the iris diaphragm should be
slowly closed until the details of the specimen are sharply defined. If the specimen appears
dark, slightly open the diaphragm.
Please note: a smaller iris diaphragm aperture (opening) increases the contrast in the image
while a larger aperture decreases the contrast. (The diaphragm is not intended for controlling
the brightness of the illumination). A good procedure to follow in selecting the proper open-
ing is to start with a large aperture and reducing it until the fine detail of the specimen is in
exact focus. Using an inappropriate aperture results in a “washing out” of the image. Care
must be exercised not to reduce the aperture too much to gain high contrast, as then the fine
structure in the image of the specimen will be destroyed. Reducing the aperture increases
contrast and depth of focus, but it also reduces resolution and causes diffraction. Example:
The aperture for the 10X objective will not be the same as for the 40XRD objective, since the
angle of the required light is determined by the numerical aperture (N.A.) of the objective.
The proper aperture of the diaphragm can be easily achieved after minimal experience with
the microscope.
SILVER
150
SERIES
| 11
MICROSCOPIC OPERATION
SILVER
LINE
