MOLECULAR DEVICES > CLONEPIX 2
ROBOT MANUAL
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.GFT FILE
Format used to save a Process Template.
INTERIOR LIGHT
Illuminates the interior of the instrument. Can be activated or deactivated at any time using
the Interior Light icon in the bottom right corner of the screen. Interior Light is not used for
imaging and will automatically switch off during imaging
PETRIWELL-1 PLATE
Single well plate optimized for visualizing plated colonies. The non-treated type (Catalogue
number W1055) are recommended for colonies suspended in semi-solid medium. The TC-
treated type (Catalogue number W1050) are recommended for colonies grown as adherent
monolayers.
PETRIWELL-6 PLATE
Six well plate optimized for visualizing plated colonies. The non-treated type (Catalogue
number W1155) are recommended for colonies suspended in semi-solid medium. The TC-
treated type (Catalogue number W1150) are recommended for colonies grown as adherent
monolayers.
PICKING PINS
Reusable stainless steel tools used to collect colonies. F1 pins have an internal tip diameter of
400µm and are for picking suspended colonies. F2 pins have an internal tip diameter of
700µm and are for picking adherent colonies (monolayers).
PRIMARY PIN
The picking pin nearest the front of the robot. All other pins are aligned relative to this one.
PRIME CONFIGURATION
The Acquisition Option that will be used for colony detection. Normally this is based on white
light imaging.
PRIME PROBE
The type of illumination that will be used for colony detection. Normally, this will be white
light by transillumination (trans). The Prime Probe is set up by creating a Prime
Configuration.
PROCESS
A standard program for ClonePix 2 to carry out a task such as a series of similar experiments,
or a maintenance task. A Process is saved as a .gfp file.
PROXIMITY INDICATORS
Red lines between colonies on the image that show the nearest neighbour for each colony.
Note: This feature is not the same as the colony exclusion feature in Groups.
Remote Data Viewer
An optional software program (order code: SL4950-A01) for accessing ClonePix 2 results
remotely. Please contact Customer Support for further information.
SEMI-SOLID MEDIUM
A viscous cell culture medium such as CloneMedia used to immobilize cells in suspension to
permit growth of clonal colonies.
SOURCE PLATE
One- or 6-well microplate(s) containing colonies for picking.
