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Remaining liquid in the funnel or in the syringe

The test was uncomplete, e.g. due to a power issue 

during the process. Restart the sanitization from the 

start

12.  Operator & equipment

safety instructions

Please refer to the Operator & Equipment instructions addendum 

on 

SigmaAldrich.com/Milliflex-Oasis

13.   Technical  Assistance

For more information visit 

SigmaAldrich.com/techservice

Standard Product Warranty

The applicable warranty for the products listed in this publication may be found at: 

SigmaAldrich.com/terms (within the “Terms and Conditions of Sale” applicable  

to your purchase transaction).

Technical Assistance

For more information, go to SigmaAldrich.com/techservice.

Summary of Contents for Milliflex Oasis

Page 1: ...Oasis system Important For an animated interactive user guide visit SigmaAldrich com Milliflex Oasis for a visual understanding of the different operations The life science business of Merck operates...

Page 2: ...its 9 3 2 Media plates 10 3 3 Testing 10 3 4 Waste 13 4 Cleaning maintenance 14 4 1 Cleaning maintenance table 14 4 2 Surface sanitizers and internal flow sanitizers table 14 4 3 Filtration head clean...

Page 3: ...se water following deionization for microelectronics and for drinking water and beverages including beer wine fruit juice soft drinks as well as their raw materials The Milliflex Oasis system for micr...

Page 4: ...UK item MMPWRSPUK Japan item MMPWRSPJP USA item MMPWRSPUS South Africa item MMPWRSPZA 3 Milliflex Oasis internal flow sanitization kit item MMSANKIT1 Optional For higher throughput capability it is p...

Page 5: ...kit with plug and gasket removal tool 2 Vacuum gauge The Milliflex Oasis filtration unit package contains 1 Three bags of eight filtration units The Milliflex Oasis media plate package contains 1 Six...

Page 6: ...t Off No tension or standby The switch is touch sensitive Use your fingertip to activate it Touch the center of the switch without touching the outer ring When the Milliflex Oasis pump is on standby m...

Page 7: ...e pump should be placed at least at 160 mm 6 3 in from the front edge of the LFH see picture Figure 7 the front of the pump should be placed at least at 160 mm 6 3 in from the front edge of a horizont...

Page 8: ...yellow covers Optional From the accessory bag select two colored rings clean them using a wipe and one of the sanitizers recommended in the cleaning maintenance section then install them on the pump h...

Page 9: ...ion units Grab one end of the tape and pull it Repeat the operation if more than one box of filtration units is needed Clean each bag using a wipe moistened with one of the surface sanitizers listed i...

Page 10: ...ur fingertip Note If starting a filtration without a filtration unit placed on the filtration head you will hear a whistle noise this is due to a check valve found in each pump head and is perfectly n...

Page 11: ...l integrity In addition it helps in removing bubbles or folds between the membrane and the agar after membrane transfer Open the pre opened media plate with one hand Note Be careful not to touch the a...

Page 12: ...pty filtration unit trays can be used to transport and to incubate the media plates membrane assembly When placing media plates membrane assemblies on the trays the lid tab must be positioned out of t...

Page 13: ...ltered liquid remains in the pump head it is possible to remove it with a dry sterile wipe Regardless of the amount of liquid it does not impact the test results 3 4 Waste After use the filtration uni...

Page 14: ...nised water solution 30 Consult our local technical support Ready to use Ethanol 70 N A Ready to use Quaternary ammonium Benzalkonium chloride between 0 25 0 5 Poly hexamethylene biguanide hydrochlori...

Page 15: ...eads and clean them using a wipe moistened with a surface sanitizer listed in the cleaning maintenance section then put them back in place 4 4 Pump surface cleaning Clean the exterior of the pump the...

Page 16: ...reen Figure 33 trigger is rotated counter clockwise Take one sanitization funnel assembly sanitization kit consumable and remove its support then place the funnel on the kit Take a syringe sanitizatio...

Page 17: ...hed off repeat the operation for the second flow path Discard the sanitization kit consumables Remove the sanitization kit and clean it using a wipe moistened with one of the surface sanitizers listed...

Page 18: ...ool Clean the stainless steel pump head carefully including the grooves using a wipe moistened with one of the surface sanitizers listed in the cleaning maintenance section Clean the gaskets and the c...

Page 19: ...oistened with sanitizer Put the new gaskets and the check valve in place using the gasket removal tool details in previous chapter 4 10 Sanitization kit gaskets spare parts replacement Materials 1 San...

Page 20: ...l flow prewetting step 1 Open the funnel lid pour purified water up to the 250 mL indication level put back the lid on place 2 Touch the switch to start the filtration 3 Stop the filtration when it is...

Page 21: ...MMPWRSPIN UK item MMPWRSPUK Japan item MMPWRSPJP USA item MMPWRSPUS South Africa item MMPWRSPZA Optional Milliflex Oasis T connectors for tubing item MMTCNNECT 6 1 2 Three pumps connected Three Milli...

Page 22: ...the working area with the two rubber fasteners Figure 46 the power supply can be attached outside the working area with the rubber fasteners Clean the exterior of the pumps and the tubing using a wipe...

Page 23: ...section Install the pump heads on the pump bodies Lock the pump heads by turning clockwise until the sign on the head is aligned with the one on the pump body Figure 47 the sign on a locked head is a...

Page 24: ...unnels without membranes item MMRECVERY in the working area Remove the media plate cover Remove the funnel assembly support Place the funnel assembly on the media plate Make sure the funnel assembly i...

Page 25: ...aeruginosa Milliflex Cassette MacConkey agar for detection of lactose fermenting bacteria and coliforms Milliflex empty cassettes Empty cassettes are available for those users who would like to prepar...

Page 26: ...e medium into the cassette Use the entire content of the ampule 3 Note Avoid pressing the cassette on the working surface while inserting the media since this might compress the pad and slow the intro...

Page 27: ...0 mL funnel 57 mm 2 2 in Height 250 mL funnel 113 mm 5 5 in Maximal diameter 82 mm 3 2 in 2D code identification Datamatrix can be read with standard 2D reader Sterilization E beam irradiation Membran...

Page 28: ...lliflex Oasis Tryptic Soy media plates for detection of aerobic and anaerobic flora 48 MMSMCTS48 Milliflex Oasis Sabouraud Dextrose media plates for detection of Yeast Molds 48 MMSMCSD48 Milliflex cas...

Page 29: ...for one to three filtration pumps South Africa 1 MMPWRSPZA Milliflex Oasis filtration head 1 MMHEADMM1 Milliflex Oasis filtration head gaskets replacement kit including gaskets removal tool 5 MMGASKT...

Page 30: ...tep and continue with the remaining volume s Alternatively the user must ensure that the filter is always covered by 25 mL to 50 mL of liquid while filtering Filtration unit leakage The maximal sample...

Page 31: ...r Equipment instructions addendum on SigmaAldrich com Milliflex Oasis 13 Technical Assistance For more information visit SigmaAldrich com techservice Standard Product Warranty The applicable warranty...

Page 32: ...or Technical Service visit SigmaAldrich com techservice SigmaAldrich com Merck KGaA Frankfurter Strasse 250 64293 Darmstadt Germany VM_UG3243EN Ver 1 0 18737 12 2020 2020 Merck KGaA Darmstadt Germany...

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