M.I. P/N 004-2163 Rev. C
9
5. Alpha correction
If stroke volume comparison for alpha correction will be used, this may be done at any
point according to the protocol. If raw data will be recorded as volume units (ml or
μ
l),
alpha calculation should be performed before the final volume calibration. Recalibrate the
volume signal channels now if alpha is used.
6. Record and export data as desired
Typical protocols may include baseline data, occlusion data, stimulus reaction data, and
saline bolus data. Smaller animals should have saline bolus maneuvers performed at the
end of the study in case the saline induces detrimental effects on the cardiovascular system.
7. Analyze the data in PVAN
Ultra
If data were recorded in conductance units, PVAN will need resistivity (rho), segment
length and (if desired) alpha parameters.
Overview of a typical small animal procedure
1. Warm-up and calibration
Place the cuvette well in warm water or under the animal warming mat to warm the
plastic. This will minimize temperature effects on the resistivity measurement.
Gettin
g
Started
