Cellicon
TM
Perfusion Solution for Process Development User Guide
20
5. Locate a new filter and place it in the
holder.
6. Use a tubing welder to connect the
new filter to the tubing lines that were
designated for feed, retentate, and
permeate.
7. Unclamp the feed and retentate lines, but
ensure that the perfusate (permeate) is
clamped.
8. Prime the system again. If using a
peristaltic pump, run at a low flow rate to
avoid causing shear damage to the cells
in the line.
9. Restart the recirculation pump by
selecting
START PUMP
on the main
menu. Ensure that the flow rate has
reached the desired setpoint.
10. Restart the perfusate (permeate) pump.
Troubleshooting
System Troubleshooting
The following chart can be used to help identify issues related to system operation.
Symptom
Corrective Action
No power
Plug in system and press power button
System
component
appears light grey
and does not read
values
Connect cables from component to controller
Install correct software
Component is in
an alarm state,
appears red or
yellow
Plug in component cables
Pump alert: Place pump head into motor
Flow alert: Place tubing into flowmeter and
prime tubing
Adjust flow alarm ranges
Follow instructions provided for specific alert
Assembly does
not fit into holder
Follow order of operations for installation found
in user guide
Pump head does
not fit into motor
Pull out locking pin during installation and move
barb from covering green area to covering red
area
Place pump head facing upwards
System does not
allow changes
Check login credentials and user permissions
Disable “When LAN Accessibility is enabled,
console control is disabled” setting under
Settings->External Interfaces->LAN Accessibility
System will not
prime
Use external peristaltic pump to start flow
Choose peristaltic pump appropriate for the
tubing size
Ensure dip-tube is open and submerged in liquid
No pinching or kinking of tubing lines, welds are
open
