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Cellicon

TM

 Perfusion Solution for Process Development User Guide 

15

Choose Pump Behavior on Flow Error

Speed Control 

These define the parameters which take 

effect when the system is running in speed 

setpoint mode. Speed setpoint mode is 

activated by moving the toggle on the main 

screen to show 

Speed Setpoint

 highlighted 

by a dark grey box.
The minimum and maximum speed define 

the range of pump RPM that the system 

will allow. These settings take effect while 

running in speed setpoint mode. The pump 

speed ramp defines the rate of change when 

a new setpoint is selected. 
The 

out of tolerance

 alert will be activated when 

the actual speed does not reach the setpoint 

within the time defined in 

Out of tolerance alert 

delay

. The 

out of tolerance

 notification can be 

activated under Settings

 Alerts 

 General 

 

Out-of-Setpoint Tolerance Alerts.

External Interfaces 

Settings

•  LAN Accessibility
•  Remote Control by PLC
•  PLC Analog Input
•  PLC Digital Input
•  PLC Analog Output

User Accounts Settings

The administrator can generate, delete 

and edit user accounts, including password 

protection capabilities and rescrict access 

permissions. 
The administrator can define up to six 

user accounts. After selecting the 

+ Add 

new user

 button, enter a username and 

password, then press 

Set permissions

. The 

following permissions can be activated or 

deactivated: 
•  Controller control – User can start/stop 

the pump, change the setpoint, zero 

adjust, and view priming instructions.  

•  Settings access – User can adjust all 

settings.

•  Data log access – User can turn on/off 

data logging.

Create a New User

Set Permissions for New User

The active user can be changed by selecting 

the user account icon in the top right region 

of the screen and selecting 

Switch user

When the screen is locked, any user account 

can be selected to access the controller.
Only the administrator can change 

permissions for each user, but users can 

change their password when signed into their 

account.

User Accounts Settings from a Non-

administrator Account

Access Denied Due to User Permissions

Summary of Contents for Milipore Cellicon Perfusion Solution for Process Development

Page 1: ...n Solution for Process Development User Guide Contents Introduction 2 System Components 2 Setting Up the System 3 Starting the System 4 User Interface 4 Settings 7 Running the Pump 7 Alerts 9 Settings...

Page 2: ...scribes the installation setup and use of the CelliconTM Perfusion Solution for Process Development This system is for industrial use only and cannot be used in a residential environment System Compon...

Page 3: ...ors on the assembly as follows Press 1 to the feed line Press 2 to the retentate line Press 3 to the perfusate permeate line Connecting the Bioreactor Use a tubing welder to connect The cell retention...

Page 4: ...on Powering up the system launches the perfusion control software The initial screen is the username selection screen After selecting the username the password screen will appear Touch the white box o...

Page 5: ...ton Selecting the Settings button opens the settings screen User Icon and Time Date Display Selecting the user icon allows the user to lock the screen or switch users Lock screen will cause the contro...

Page 6: ...low Setpoint Prime System Zero Adjust Flow Rate Speed Setpoint Pump Start Decrease Flow Rate Increase Flow Rate Status The current values state of the system components and data logging switch are dis...

Page 7: ...ton will read START PUMP If the pump is on the button will read STOP PUMP Stopping the pump will cause a confimation screen to appear Prime System The pump needs to be primed to reduce damage to cells...

Page 8: ...t be stopped A pop up will appear to confirm that the pump is stopped Flowmeter 2 does not require that the pump is stopped All sensors should be zero adjusted prior to starting a run Only one sensor...

Page 9: ...splayed Alarm Acknowledgement Alerts can be removed from the main screen by selecting Acknowledge from the alert bar or in the alert log The alert will reappear on the alert bar if it remains active 1...

Page 10: ...ill make a loud beep when the alert is activated Select Dismiss to return to the main screen then select Acknowledge on the alert bar or within the alert log The data region can still be viewed when a...

Page 11: ...Critical Alarm High Optional 1 Pressure Alarms Pressure Critical Alarm Low Optional 1 Pressure Warning Alarm Low Never 2 Pressure Warning Alarm High Never 2 Pressure Critical Alarm High Optional 1 Al...

Page 12: ...ending is fixed and generated with the date followed by the time when the logfile is created When data logging is switched On a prompt to enter a filename will appear This filename will replace the t...

Page 13: ...error The resolution of the flow signal can be improved by decreasing the full scale of the flowmeter The resolution of the flow signal is always 1 10 000 of the full scale Decreasing the full scale b...

Page 14: ...Refer to Table of Alerts for more information Critical alarms are shown in red color type 1 alert Warning alarms are shown in yellow color type 2 alert Neither critical nor warning alarms will shut d...

Page 15: ...nalog Input PLC Digital Input PLC Analog Output User Accounts Settings The administrator can generate delete and edit user accounts including password protection capabilities and rescrict access permi...

Page 16: ...s have been added to the reactor This will eliminate the possible introduction of cell shear when the system is primed see Priming Preparation Equipment Needs The following user supplied items may be...

Page 17: ...r or gas in the pump head will negatively affect pump performance and decrease the recirculation flow rate MobiusTM 3L Single use Bioreactor When using the MobiusTM single use 3L reactor the inlet to...

Page 18: ...lect Prime system on the main menu screen This will switch the controller into Speed setpoint mode and provide step by step guidance on how to complete the priming 2 Ensure that the perfusate permeate...

Page 19: ...ved on the USB drive the log interval and the log file size Choose a log interval of 60 seconds or greater and a log file size of 65000 lines Insert the USB drive into a computer to view data Open Mic...

Page 20: ...ars light grey and does not read values Connect cables from component to controller Install correct software Component is in an alarm state appears red or yellow Plug in component cables Pump alert Pl...

Page 21: ...d ensure it does not pull air from sparger Check system mode flow control vs speed control No flow through permeate Ensure permeate line is not clamped or pinched Symptom Corrective Action Early filte...

Page 22: ...un Disposing of the Filter The filter is completely single use and should be disposed of after use Follow company procedure for waste disposal Cleaning the Hardware All parts of the system should be w...

Page 23: ...s All other trademarks are the property of their respective owners Detailed information on trademarks is available via publicly accessible resources MK_UG5285EN Rev 1 06 2020 2020 Merck KGaA Darmstadt...

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