3.1 Incident Light Operation – Brightfield
1.
First, set your interpupillary distance on the
microscope eyetubes by pulling them apart or
pushing them closer together to fit your eyes. When
set correctly, one will see one uniform round or
“fused” field of view. Make note of the distance
setting when adjusted to your liking so you can later repeat the setting.
2.
Turn on the power to the fiber optic illuminator. Adjusting the brightness desired
is done with the variable brightness control knob on the front of the illuminator
power supply.
3.
Make sure the field iris diaphragm on the vertical illuminator is opened all the
way open to start.
4.
We recommend that you initially use a flat, easily recognizable specimen like a
coin to setup your microscope. Place that specimen on the stage.
5.
Select a lower power “scanning” objective like the 5X or the 10X to find the area
of interest on the specimen quickly. Be sure the objective “clicks” into place when
you turn the objective nosepiece.
6.
The aperture iris diaphragm located on the vertical illuminator can be "stopped
down" or closed somewhat to give the observation of your specimen more or less
contrast or resolving power. Stopping down the diaphragm decreases resolution
and brightness but increases image contrast and depth of focus.
Possible Brightfield Mode Operational Problems
If normal adjustments are not getting the results you expect, check to see if these
conditions exist:
•
Incorrect components inadvertently installed
•
Components not mounted flush (Vertical Illuminator to microscope frame, etc.)
•
Dirty or smudged optics
MC Series S.Plan Metallurgical Brightfield Objectives
Brightfield Objectives –
Plan Episcopic
-
Infinity Corrected - F = 200mm
MA330
S.Plan M5X objective, NA: 0.10,
WD = 20.0mm (included)
MA337
S.Plan Epi 10X objective, NA: 0.25,
WD = 9.4mm (included)
MA338
S.Plan Epi 20X, objective, NA: 0.40,
WD = 5.28mm (optional)
MA339
MA328
S.Plan Epi 40X objective, NA: 0.65,
WD = 0.81mm (included)
S.Plan Epi 100X objective, NA: 0.75,
WD = 0.27mm (optional)
