Lonza PAGEr Minigel Chamber Instruction Manual Download Page 6

 

 

6

2, Greene Publishing Associates, Inc. and John 
Wiley & Sons, Inc., Ch. 

10

 

 

SECTION 6 
Maintenance of Equipment 
6.1 

Care and Handling 

The plastic components of the PAGEr

 Minigel 

Chamber are fabricated from polycarbonate.  Electrodes 
and connectors are made from pure platinum, stainless 
steel, and nickel plated brass.  As with any laboratory 
instrument, adequate care ensures consistent and 
reliable performance. 
 

After each use, rinse all parts with de-ionized water.

  

Wipe dry with a soft cloth or paper towel, or allow to air 
dry.  Whenever necessary, all components may be 
washed gently with water and a non-abrasive detergent, 
and rinsed and dried as above.  

Never

 use abrasive 

cleaners, glass cleaning sprays or scouring pads to 
clean the components, as these will damage the unit and 
components. 

 
Additional precautions: 

 

Do not autoclave or dry-heat sterilize the apparatus 
or components. 

 

Do not expose the apparatus or components to 
phenol, acetone, benzene, halogenated 
hydrocarbon solvents or undiluted alcohols. 

 

Avoid prolonged exposure of the apparatus or 
components to UV light. 

 

Do NOT treat with diethylpyrocarbonate (DEPC)-
treated water for extended periods at 37ºC.  A brief 
rinse with DEPC-water is sufficient after a thorough 
wash, followed by a quick rinse in 70% ethanol. 

 
6.2  

Maintenance

 

The following inspection and maintenance procedures 
will help maintain the safety and reliable performance of 
the PAGEr Minigel Chamber.  Replacement parts can be 
requested by calling 1-800-638-8174. 

 

Banana plugs and power cords should be inspected 
regularly.  If the banana plugs become loose or do 
not feel friction tight replace the plugs or power 
cords.   

 

Should power cord assemblies (connectors, wire or 
shrouds) show any signs of wear or damage (e.g. 
cracks, nicks, abrasions, or melted insulation), 
replace them immediately. 

 

The platinum wire is secured to the banana jack by 
compression between a stainless washer and the 
jack nut. The nut/washer interface should be tight 
and free of corrosion.   

 
 
 
 
 
 

 
Ordering Information  

 

 Cat No

Description

59905 

PAGEr Minigel Chamber 

59906 

PAGEr™ Blot Module 

59907 

PAGEr Minigel Chamber and  
Blot Module Kit 
(Includes chamber, blotting cassette and 
sponge pads) 

50879 AccuGENE

®

 10X Tris-Glycine Buffer  1L 

50881 

AccuGENE 10X Tris-Glycine Buffer  4L 

50880 

AccuGENE 10X Tris-Glycine SDS Buffer  1L 

50882 

AccuGENE 10X Tris-Glycine SDS Buffer  4L 

 
For Research Use Only.  Not for Use in Diagnostic 
Procedures. 

 
 
 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
PAGEr and AccuGENE, are trademarks of Lonza Group or its 
subsidiaries.  
 

©

2007 Lonza Rockland, Inc.  

All rights reserved.

 

 

                                    

 

 

 

Summary of Contents for PAGEr Minigel Chamber

Page 1: ...ct proves defective during this period Lonza Rockland Inc will repair or replace it at our option free of charge if returned to us postage prepaid This warranty does not cover damage in transit damage caused by carelessness misuse or neglect normal wear through frequent use damage caused by solvent corrosion damage caused by improper handling or user alteration nor unsatisfactory performance as a ...

Page 2: ...running single gels not shown 2 adaptors for running 9 cm gels not shown Section 3 Instructions for Electrophoresis 3 1 Preparing the Electrophoresis Unit 1 Place unit in authorized work area Remove safety cover from the assembled unit by simultaneously pressing down on white push pins with your thumbs while lifting up on yellow safety cover with your fingers Do not remove safety cover by pulling ...

Page 3: ...mpressed air Failure to do this will result in accelerated banana jack corrosion Banana jack receptacles Figure 6 Figure 4 2 See section 5 3 for recommended buffers Pour only enough freshly prepared buffer into lower chamber so that the final buffer level is just below bottom of sample wells Using a pipette or syringe thoroughly flush out the wells 3 Load samples If outer lanes do not contain samp...

Page 4: ...X Towbin 1X tris glycine buffer 20 Methanol buffer for 10 minutes b Cut a piece of PVDF or nitrocellulose membrane to fit the gel Do Not Touch Membrane with Bare Hands Wear gloves c Soak membrane in 50 methanol for 30 seconds d Soak membrane in water for 5 minutes e Soak membrane 5 minutes in 1X Towbin buffer until it no longer floats Do Not Allow Membrane to Dry f Cut 1 piece of filter paper 1 mm...

Page 5: ...nditions will vary according to the number and type of gels used buffer conditions employed power input and the general goal of the experiment Refer to section 5 4 for in depth discussions on practical and theoretical approaches to protein gel electrophoresis Using standard SDS PAGE buffer systems see section 5 3 For two 1 0mm thick gels at room temperature use the following conditions at constant...

Page 6: ... brief rinse with DEPC water is sufficient after a thorough wash followed by a quick rinse in 70 ethanol 6 2 Maintenance The following inspection and maintenance procedures will help maintain the safety and reliable performance of the PAGEr Minigel Chamber Replacement parts can be requested by calling 1 800 638 8174 Banana plugs and power cords should be inspected regularly If the banana plugs bec...

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