Lonza PAGEr Minigel Chamber Instruction Manual Download Page 2

 

 

2

Maximum Limits

250 VDC 
30 watts power 
150 mA current 
60ºC ambient temperature 

 

 

Current to the unit, provided from the external power 
supply, must enter the unit through the lid assembly, 
providing a safety interlock to the user.  Current to the 
unit is broken when the lid is removed. 

  

Do not attempt to use the unit without the safety lid, 
and always turn the power supply off before 
removing the lid, or when working with the unit in 
any way.   Follow safety precautions specified by the 
power supply manufacturer.  
 
Important: After each use, rinse all parts with  
de-ionized water. 
 
SECTION 2  
Description of Parts 
2.1   

Unpacking and Components    

Please verify that your unit comes complete with the 
following components: 
• 

Lower reservoir  

• 

Safety cover with attached DC power leads 

• Core 
• 

1 blank plate for running single gels (not shown) 

• 

2 adaptors for running 9 cm gels (not shown) 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

Section 3   
Instructions for Electrophoresis  
 
3.1 

Preparing the Electrophoresis Unit 

 

1.  Place unit in authorized work area. Remove safety 

cover from the assembled unit by simultaneously 
pressing down on white push pins with your thumbs, 
while lifting up on yellow safety cover with your 
fingers.  

Do not remove safety cover by pulling up 

on leads!

 

 
 
 
 
 
 
 
 
 
 
 

 
 
 
 
 

Figure 2 

 
 
2.  Remove white core from lower reservoir by grasping 

core with one hand and lifting directly up. 

NOTE: If desired, gels may be inserted without 
removing the core from the reservoir. 
 

 
 
 
 

 
 
 
 
 
 
 
 
 
 

Figure 3 

Figure 1 

3.  Open doors on the core assembly by pulling up on 

the white latches. 

 
4.  Slide pre-cast gel cassette into the core assembly 

with the notched plate facing in towards the upper 
buffer reservoir as shown in figure 3.  

 
5.  If running one gel, slide the blank plate into the side 

without the gel. If running a 9 cm x 10 cm  cassette, 
insert the 9 cm gel adapter, as shown in figure 4. 

Summary of Contents for PAGEr Minigel Chamber

Page 1: ...ct proves defective during this period Lonza Rockland Inc will repair or replace it at our option free of charge if returned to us postage prepaid This warranty does not cover damage in transit damage caused by carelessness misuse or neglect normal wear through frequent use damage caused by solvent corrosion damage caused by improper handling or user alteration nor unsatisfactory performance as a ...

Page 2: ...running single gels not shown 2 adaptors for running 9 cm gels not shown Section 3 Instructions for Electrophoresis 3 1 Preparing the Electrophoresis Unit 1 Place unit in authorized work area Remove safety cover from the assembled unit by simultaneously pressing down on white push pins with your thumbs while lifting up on yellow safety cover with your fingers Do not remove safety cover by pulling ...

Page 3: ...mpressed air Failure to do this will result in accelerated banana jack corrosion Banana jack receptacles Figure 6 Figure 4 2 See section 5 3 for recommended buffers Pour only enough freshly prepared buffer into lower chamber so that the final buffer level is just below bottom of sample wells Using a pipette or syringe thoroughly flush out the wells 3 Load samples If outer lanes do not contain samp...

Page 4: ...X Towbin 1X tris glycine buffer 20 Methanol buffer for 10 minutes b Cut a piece of PVDF or nitrocellulose membrane to fit the gel Do Not Touch Membrane with Bare Hands Wear gloves c Soak membrane in 50 methanol for 30 seconds d Soak membrane in water for 5 minutes e Soak membrane 5 minutes in 1X Towbin buffer until it no longer floats Do Not Allow Membrane to Dry f Cut 1 piece of filter paper 1 mm...

Page 5: ...nditions will vary according to the number and type of gels used buffer conditions employed power input and the general goal of the experiment Refer to section 5 4 for in depth discussions on practical and theoretical approaches to protein gel electrophoresis Using standard SDS PAGE buffer systems see section 5 3 For two 1 0mm thick gels at room temperature use the following conditions at constant...

Page 6: ... brief rinse with DEPC water is sufficient after a thorough wash followed by a quick rinse in 70 ethanol 6 2 Maintenance The following inspection and maintenance procedures will help maintain the safety and reliable performance of the PAGEr Minigel Chamber Replacement parts can be requested by calling 1 800 638 8174 Banana plugs and power cords should be inspected regularly If the banana plugs bec...

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