Lonza PAGEr Minigel Chamber Instruction Manual Download Page 1

 

Lonza Rockland, Inc. 
www.lonza.com 
[email protected] 
Tech Service: 800-521-0390 
Customer Service: 800-341-1574 
Document # 18928-0305-01 
Rockland, ME 04841 USA

 
 
 
 
 

 

IMPORTANT USER INFORMATION 
This Instruction Manual will explain how to use this 
product safely and effectively.  Please read and 
carefully follow the instruction manual in its entirety.   

 

The triangle/exclamation mark symbol 
alerts the user of the product to important 
operational, maintenance, and/or warranty 
requirements.   
 
The triangle/lightning bolt symbol alerts the 
user of the product to potentially hazardous 
electrical exposure. 

 
Failure to adhere to the instructions could result in 
personal and/or laboratory hazards, as well as invalidate 
any warranty. Always turn off the DC power source prior 
to disconnecting power cords from the product.  
Disconnect power cords from the power source first and 
then from the product. For maximum safety, always 
operate this system in an isolated, low traffic area, not 
accessible to unauthorized personnel. Never operate 
damaged or leaking equipment. 
 

WARRANTY AND LIABILITY 

This product was produced utilizing the highest practical 
standards of materials, workmanship, and design.  
Lonza Rockland, Inc. warrants that the product has been 
tested and will meet or exceed published specifications.  
This warranty is valid only if the product has been 
operated and maintained according to the instructions 
provided. 
 
Lonza Rockland, Inc., warrants this product to be free 
from defects in materials and workmanship under normal 
service for one year from date of shipment.  If the 
product proves defective during this period, Lonza 
Rockland, Inc., will repair or replace it at our option, free 
of charge, if returned to us postage prepaid.  This 
warranty does not cover: damage in transit, damage 
caused by carelessness, misuse or neglect, normal wear 
through frequent use, damage caused by solvent 
corrosion, damage caused by improper handling or user  

 
alteration, nor unsatisfactory performance as a result of 
conditions beyond our control.  Lonza Rockland, Inc., 
shall in no event be liable for incidental nor 
consequential damages, including without limitation, lost 
profits, loss of income, loss of business opportunities, 
loss of use and other related damages, however caused, 
nor any damage arising from the incorrect use of the 
product.   

 
SECTION 1 
General Information  
 
1.1 Introduction 

The PAGEr Minigel Chamber is designed for optimal 
performance of PAGEr

® 

Precast Gels (Lonza).   

The unit is also compatible with many standard precast 
minigels. The simple lock-in-place core design assures a 
tight, flat fit and eliminates the risk of buffer leaks.  There 
is no need to remove the core from the reservoir, simply 
insert the gels, close the clamps, fill with buffer and run.  
The chamber can run one or two gels and 
accommodates a tank blotting module.   

 
Table 1: Components of the PAGEr Minigel Chamber

 

 

 Cat No

Description

59905 

PAGEr Minigel Chamber 

59906 

PAGEr™ Blot Module 

59907 

PAGEr Minigel Chamber and  
Blot Module Kit 
(Includes chamber, blotting 
cassettes and sponge pads) 

 
1.2 Safety 

Power to the PAGEr Minigel Chamber is to be supplied 
by an external DC voltage power supply that must be 
ground isolated so that the DC voltage output floats with 
respect to ground.  For any power supply used, the 
maximum specified operating parameters for the units 
are: 
 
 

PAGEr™ Minigel Chamber 

 

 

1

Summary of Contents for PAGEr Minigel Chamber

Page 1: ...ct proves defective during this period Lonza Rockland Inc will repair or replace it at our option free of charge if returned to us postage prepaid This warranty does not cover damage in transit damage caused by carelessness misuse or neglect normal wear through frequent use damage caused by solvent corrosion damage caused by improper handling or user alteration nor unsatisfactory performance as a ...

Page 2: ...running single gels not shown 2 adaptors for running 9 cm gels not shown Section 3 Instructions for Electrophoresis 3 1 Preparing the Electrophoresis Unit 1 Place unit in authorized work area Remove safety cover from the assembled unit by simultaneously pressing down on white push pins with your thumbs while lifting up on yellow safety cover with your fingers Do not remove safety cover by pulling ...

Page 3: ...mpressed air Failure to do this will result in accelerated banana jack corrosion Banana jack receptacles Figure 6 Figure 4 2 See section 5 3 for recommended buffers Pour only enough freshly prepared buffer into lower chamber so that the final buffer level is just below bottom of sample wells Using a pipette or syringe thoroughly flush out the wells 3 Load samples If outer lanes do not contain samp...

Page 4: ...X Towbin 1X tris glycine buffer 20 Methanol buffer for 10 minutes b Cut a piece of PVDF or nitrocellulose membrane to fit the gel Do Not Touch Membrane with Bare Hands Wear gloves c Soak membrane in 50 methanol for 30 seconds d Soak membrane in water for 5 minutes e Soak membrane 5 minutes in 1X Towbin buffer until it no longer floats Do Not Allow Membrane to Dry f Cut 1 piece of filter paper 1 mm...

Page 5: ...nditions will vary according to the number and type of gels used buffer conditions employed power input and the general goal of the experiment Refer to section 5 4 for in depth discussions on practical and theoretical approaches to protein gel electrophoresis Using standard SDS PAGE buffer systems see section 5 3 For two 1 0mm thick gels at room temperature use the following conditions at constant...

Page 6: ... brief rinse with DEPC water is sufficient after a thorough wash followed by a quick rinse in 70 ethanol 6 2 Maintenance The following inspection and maintenance procedures will help maintain the safety and reliable performance of the PAGEr Minigel Chamber Replacement parts can be requested by calling 1 800 638 8174 Banana plugs and power cords should be inspected regularly If the banana plugs bec...

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