logos biosystems LUNA-II User Manual Download Page 31

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Chapter 9 - Purchaser Notification   

9.1 Limited Use Label License: Research Use Only 

The purchaser of this product should use this product only for research for the sole benefit of the purchaser. By use of this 
product, the purchaser agrees to be bounded by the terms of this limited use statement whether the purchaser is a for-
profit or a not-for-profit entity. 

If the purchaser is not willing to accept the conditions of this limited use statement and this product is unused, the Company 
will accept return of the product with a full refund. 

The  purchaser  cannot  resell  or  otherwise  transfer  (a)  this  product  (b)  its  components  or  (c)  materials  made  using  this 
product or its components to a third party for Commercial Purposes. 

Commercial  Purposes  mean  any  and  all  uses  of  this  product  and  its  components  by  a  party  for  monetary  or  other 
consideration,  including  but  not  limited  to,  (a)  product  manufacture,  (b)  providing  a  service,  information,  or  data,  (c) 
therapeutic,  diagnostic,  or  prophylactic  purposes,  or  (d)  resale  of  this  product  or  its  components  whether  or  not  such 
product and its components are resold for use in research. 

Logos Biosystems, Inc. (“Company”) will not  claim any consideration against the purchaser of infringement of patents 
owned or controlled by the Company which cover the product based on the manufacture, use or sale of a therapeutic, 
clinical diagnostic, vaccine, or prophylactic product developed in research by the purchaser in which this product or its 
components was employed, provided that neither this product nor any of its components was used in the manufacture of 
such product.   

For  any  use  other  than  this  limited  use  label  license  of  research  use  only,  please  contact  the  Company  or  email 
[email protected] for more information. 

 

9.2 Instrument Warranty 

 

Logos Biosystems, Inc. (“Company”) warrants to the original purchaser (“Purchaser”) that the instrument (“Instrument”), if 
properly  used  and  installed,  will  be  free  from  defects  in  materials  and  workmanship  and  will  conform  to  the  product 

specifications for a period of one (1) year (“Warranty Period”) from the date of purchase. If the Instrument under this limited 
warranty fails during the Warranty Period, the Company, at its sole responsibility, will: 

1)  within and up to 30 calendar days of purchase, refund the purchase price of the Instrument to the Purchaser if 

the Instrument is in original conditions; or, 

2)  after 30 calendar days of purchase, only replace or repair the Instrument for up to the Warranty Period without 

issuing a credit. 

In no event shall the Company accept any returned instrument (including its components) that might have been used or 
contaminated in some labs, including but not limited to, HIV or other infectious disease or blood-handling labs. This limited 
warranty does not cover refund, replacement, and repair incurred by accident, abuse, misuse, neglect, unauthorized repair, 
or modification of the Instrument. This limited warranty will be invalid if the Instrument is disassembled or repaired by the 
Purchaser. 

In case that the Company decides to repair the Instrument, not to replace, this limited warranty includes replacement parts 
and labor for the Instrument. This limited warranty does not include shipment of the Instrument to and from service location 
or  travel  cost  of  service  engineer,  the  costs  of  which  shall  be  borne  by  the  Purchaser.  Every  effort  has  been  made  to 
ensure that all the information contained in this document is correct at its publication. However, the Company makes no 
warranty  of  any  kind  regarding  the  contents of  any  publications or  documentation  as  unintended  or  unexpected  errors 
including occasional typographies or other kinds are inevitable. In addition, the Company reserves the right to make any 
changes  necessary  without  notice  as  part  of  ongoing  product  development.  If  you  discover  an  error  in  any  of  our 
publications, please report it to your local supplier or the Company. The Company shall have no responsibility or liability 
for any special, incidental, indirect or consequential loss or damage resulting from the use or malfunction of the Instrument.   

This limited warranty is sole and exclusive. The Company makes no other representations or warranties of any kind, either 
express or implied, including for merchantability or fitness for a particular purpose with regards to this Instrument. To obtain 

service during the Warranty Period, contact your local supplier or the Company’s Technical Support team. 

OUT OF WARRANTY SERVICE 

Please contact your local supplier or the Company’s technical support team in order to obtain out-of-warranty service. If 
necessary,  repair  service  will  be  charged  for  replacement  parts  and  labor  hours  incurred  to  repair  the  Instrument.  In 
addition,  the  Purchaser  is  responsible  for  the  cost  of  shipping  the  Instrument  to  and  from  the  service  facility  and,  if 
necessary, the travel cost of a service engineer. 

Summary of Contents for LUNA-II

Page 1: ...User Manual www logosbio com LBSM MD ML LUC 001 VL1712 01...

Page 2: ...oratory instrument for scientific research use only It is not a medical therapeutic or in vitro diagnostics device Do not disassemble the device on any occasion as this will invalidate your warranty T...

Page 3: ...col Parameters 11 3 2 Creating and Editing Protocols 12 3 3 Protocol Selection 13 Chapter 4 Counting Cells 14 4 1 Instrument Preparation 14 4 2 Sample Preparation 14 4 3 Slide Insertion 15 4 4 Focusin...

Page 4: ...ned for cost efficient an accurate cell counting the LUNA Reusable Slide combines the economy of manual cell counting with the speed accuracy and convenience of automated cell counting The disposable...

Page 5: ...ation This is the minimum processing time for each focusing option at the specified concentration of HeLa or HL 60 cells Component Quantity LUNA II Automated Cell Counter 1 Power Cord with AC adapter...

Page 6: ...elines Follow the instructions below to obtain the best results with the LUNA II Automated Cell Counter 1 Hold slides by the edges to avoid touching the optical surface Take care that the optical surf...

Page 7: ...o not install the instrument in a location that will expose the instrument to intense ultraviolet light 2 2 Startup Main Menu Push the power button below the touchscreen to turn the instrument on The...

Page 8: ...and values of the most recent calibration and the date and version of the latest software update Settings options 2 3 1 Staining Options Select for the presence or absence of a stain 2 3 2 Counting Op...

Page 9: ...on factor will not change automatically Failure to adjust the dilution factor will lead to inaccurate cell concentration calculations Low contrast from not using a stain may lead to suboptimal results...

Page 10: ...Date Time in the Settings screen Select the desired field to delete the existing value Input the desired values with the number panel on the right Press Apply to save changes Press to return to the S...

Page 11: ...e in the counting slide port remove it from the instrument Press Start Do not turn the instrument off during this process Mix one part stain with an equal volume of distilled water PBS or plain medium...

Page 12: ...itive and not detect weakly stained cells With lower noise reduction the instrument can detect objects with faint signals Adjusting this parameter will help optimize for different cell types as staini...

Page 13: ...nnot be modified or deleted To create a new protocol select New Protocol and press Load Press Delete to delete the selected protocol Press Edit to modify the selected protocol This will activate the a...

Page 14: ...tocol screen Press Load to apply the selected protocol Now the instrument is ready to count cells with the selected protocol Important Merely selecting a protocol does not mean that it has been put in...

Page 15: ...ration Prepare a cell suspension according to standard procedures Mix gently but thoroughly to ensure that the suspension is homogenous Mix 10 L cell suspension with 10 L stain Pipette gently Prepare...

Page 16: ...g algorithm that works in tandem with a focusing mechanism to rapidly obtain the Z position of the sample by applying a small voltage to a liquid lens The elimination of mechanical parts in the focusi...

Page 17: ...screen Press the magnifier button to zoom in and out of the image Press Count to start counting The LUNA II counts the cells in 0 5 L which is comparable to five 1 mm x 1 mm squares on a standard hemo...

Page 18: ...d cell sample Use a finger or a stylus to navigate the image The Tag and magnifier buttons are to the right of the image Press the magnifier button to zoom in and out of the saved image Press Tag to l...

Page 19: ...cells according to their sizes Green bars represent live cells and red bars represent dead cells The Total on button indicates that live and dead cells are both represented Press Total on to change i...

Page 20: ...he size to alter the minimum and maximum size limits The gating function is helpful for monitoring co cultured cells with distinct sizes and the exclusion of noncellular particles Press Apply to set t...

Page 21: ...of total cells live and dead as the current concentration The current concentration options are Total Live Dead and Custom allowing users to set the current concentration to be the total cell concentr...

Page 22: ...ged image of live and dead cells Raw Image Untagged image of cells Report PDF report with count data and histograms Select the desired saving options The selected options will be marked with a blue Us...

Page 23: ...22 HL60 pdf HL60_RAW tif HL60_TAG tif...

Page 24: ...ts and protocol details Cell Count Report File name HL60 Date 16 Apr 2017 14 35 Cell count results Total 1 04x10e6 cells mL Live 9 73x10e5 cells mL Dead 6 62x10e4 cells mL Viability 93 6 Avg size 16 5...

Page 25: ...a USB drive into the LUNA II Press Review Files to select a LUNA II generated folder from the USB drive The cell count results and corresponding image will appear on the right side of the screen If av...

Page 26: ...during cleaning Clean the surfaces of the instrument with a soft cloth dampened with distilled water Wipe dry immediately Do not pour or spray liquids directly onto the instrument Do not wet electrica...

Page 27: ...Data transfer and saving Incompatible USB drive Some USB devices are undetectable or incompatible Use the USB supplied with the instrument or use a USB 2 0 Too many files in the USB drive Delete or tr...

Page 28: ...anteed 8 Turn the instrument on only after connecting the power cord and AC adapter to the power source and the instrument Turn the instrument off before disconnecting the power cord or moving the ins...

Page 29: ...sions for which this marking is required Users must be aware of and follow the conditions described in this manual for operating the instrument The protection provided by the instrument may be impaire...

Page 30: ...able Slide Coverslips 10 units L12001 LUNA Cell Counting Slides 50 slides 100 counts 1 box L12002 LUNA Cell Counting Slides 500 slides 1 000 counts 10 boxes L12003 LUNA Cell Counting Slides 1 000 slid...

Page 31: ...strument is in original conditions or 2 after 30 calendar days of purchase only replace or repair the Instrument for up to the Warranty Period without issuing a credit In no event shall the Company ac...

Page 32: ...31 478 4184 Email sales logosbio com USA 7700 Little River Turnpike STE 207 Annandale VA 22003 USA Tel 1 703 622 4660 Tel 1 703 942 8867 Fax 1 517 266 3925 Email sales logosbio com EUROPE 11B avenue...

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