logos biosystems LUNA-II User Manual Download Page 14 | Manualshive

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logos biosystems LUNA-II User Manual Download Page 14

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3.3 Protocol Selection

Select the desired protocol in the Protocol screen.

Press

Load

to apply the selected protocol.

Now the instrument is ready to count cells with the selected protocol.



Important!

Merely selecting a protocol does not mean that it has been put into effect. To apply the selected

protocol, make sure to press

Load

.

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Summary of Contents for LUNA-II

Page 1: ...User Manual www logosbio com LBSM MD ML LUC 001 VL1712 01...

Page 2: ...oratory instrument for scientific research use only It is not a medical therapeutic or in vitro diagnostics device Do not disassemble the device on any occasion as this will invalidate your warranty T...

Page 3: ...col Parameters 11 3 2 Creating and Editing Protocols 12 3 3 Protocol Selection 13 Chapter 4 Counting Cells 14 4 1 Instrument Preparation 14 4 2 Sample Preparation 14 4 3 Slide Insertion 15 4 4 Focusin...

Page 4: ...ned for cost efficient an accurate cell counting the LUNA Reusable Slide combines the economy of manual cell counting with the speed accuracy and convenience of automated cell counting The disposable...

Page 5: ...ation This is the minimum processing time for each focusing option at the specified concentration of HeLa or HL 60 cells Component Quantity LUNA II Automated Cell Counter 1 Power Cord with AC adapter...

Page 6: ...elines Follow the instructions below to obtain the best results with the LUNA II Automated Cell Counter 1 Hold slides by the edges to avoid touching the optical surface Take care that the optical surf...

Page 7: ...o not install the instrument in a location that will expose the instrument to intense ultraviolet light 2 2 Startup Main Menu Push the power button below the touchscreen to turn the instrument on The...

Page 8: ...and values of the most recent calibration and the date and version of the latest software update Settings options 2 3 1 Staining Options Select for the presence or absence of a stain 2 3 2 Counting Op...

Page 9: ...on factor will not change automatically Failure to adjust the dilution factor will lead to inaccurate cell concentration calculations Low contrast from not using a stain may lead to suboptimal results...

Page 10: ...Date Time in the Settings screen Select the desired field to delete the existing value Input the desired values with the number panel on the right Press Apply to save changes Press to return to the S...

Page 11: ...e in the counting slide port remove it from the instrument Press Start Do not turn the instrument off during this process Mix one part stain with an equal volume of distilled water PBS or plain medium...

Page 12: ...itive and not detect weakly stained cells With lower noise reduction the instrument can detect objects with faint signals Adjusting this parameter will help optimize for different cell types as staini...

Page 13: ...nnot be modified or deleted To create a new protocol select New Protocol and press Load Press Delete to delete the selected protocol Press Edit to modify the selected protocol This will activate the a...

Page 14: ...tocol screen Press Load to apply the selected protocol Now the instrument is ready to count cells with the selected protocol Important Merely selecting a protocol does not mean that it has been put in...

Page 15: ...ration Prepare a cell suspension according to standard procedures Mix gently but thoroughly to ensure that the suspension is homogenous Mix 10 L cell suspension with 10 L stain Pipette gently Prepare...

Page 16: ...g algorithm that works in tandem with a focusing mechanism to rapidly obtain the Z position of the sample by applying a small voltage to a liquid lens The elimination of mechanical parts in the focusi...

Page 17: ...screen Press the magnifier button to zoom in and out of the image Press Count to start counting The LUNA II counts the cells in 0 5 L which is comparable to five 1 mm x 1 mm squares on a standard hemo...

Page 18: ...d cell sample Use a finger or a stylus to navigate the image The Tag and magnifier buttons are to the right of the image Press the magnifier button to zoom in and out of the saved image Press Tag to l...

Page 19: ...cells according to their sizes Green bars represent live cells and red bars represent dead cells The Total on button indicates that live and dead cells are both represented Press Total on to change i...

Page 20: ...he size to alter the minimum and maximum size limits The gating function is helpful for monitoring co cultured cells with distinct sizes and the exclusion of noncellular particles Press Apply to set t...

Page 21: ...of total cells live and dead as the current concentration The current concentration options are Total Live Dead and Custom allowing users to set the current concentration to be the total cell concentr...

Page 22: ...ged image of live and dead cells Raw Image Untagged image of cells Report PDF report with count data and histograms Select the desired saving options The selected options will be marked with a blue Us...

Page 23: ...22 HL60 pdf HL60_RAW tif HL60_TAG tif...

Page 24: ...ts and protocol details Cell Count Report File name HL60 Date 16 Apr 2017 14 35 Cell count results Total 1 04x10e6 cells mL Live 9 73x10e5 cells mL Dead 6 62x10e4 cells mL Viability 93 6 Avg size 16 5...

Page 25: ...a USB drive into the LUNA II Press Review Files to select a LUNA II generated folder from the USB drive The cell count results and corresponding image will appear on the right side of the screen If av...

Page 26: ...during cleaning Clean the surfaces of the instrument with a soft cloth dampened with distilled water Wipe dry immediately Do not pour or spray liquids directly onto the instrument Do not wet electrica...

Page 27: ...Data transfer and saving Incompatible USB drive Some USB devices are undetectable or incompatible Use the USB supplied with the instrument or use a USB 2 0 Too many files in the USB drive Delete or tr...

Page 28: ...anteed 8 Turn the instrument on only after connecting the power cord and AC adapter to the power source and the instrument Turn the instrument off before disconnecting the power cord or moving the ins...

Page 29: ...sions for which this marking is required Users must be aware of and follow the conditions described in this manual for operating the instrument The protection provided by the instrument may be impaire...

Page 30: ...able Slide Coverslips 10 units L12001 LUNA Cell Counting Slides 50 slides 100 counts 1 box L12002 LUNA Cell Counting Slides 500 slides 1 000 counts 10 boxes L12003 LUNA Cell Counting Slides 1 000 slid...

Page 31: ...strument is in original conditions or 2 after 30 calendar days of purchase only replace or repair the Instrument for up to the Warranty Period without issuing a credit In no event shall the Company ac...

Page 32: ...31 478 4184 Email sales logosbio com USA 7700 Little River Turnpike STE 207 Annandale VA 22003 USA Tel 1 703 622 4660 Tel 1 703 942 8867 Fax 1 517 266 3925 Email sales logosbio com EUROPE 11B avenue...

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