using the iBlot
®
Gel Transfer device with Blotting Roller
©2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective
owners.
For technical support, email [email protected].
For country-specific contact information, visit www.invitrogen.com.
1. Close the lid and secure
the latch. The red light
is on indicating a closed
circuit.
2. Ensure the correct
program and time are
selected.
3. Press the Start/Stop but-
ton. The red light chang-
es to green.
4. Current automatically
shuts off at the end of
each run. The end of
transfer is indicated by
beeping sounds, and
flashing red light and
digital display. Press and
release the Start/Stop
Button. The light turns to
a steady red.
5. Place the pre-soaked (in
deionized water) iBlot
®
Filter Paper on the pre-
run gel and remove air
bubbles using the Blot-
ting Roller.
6. Unseal the Cathode
Stack, Top (shown here
for a Mini Stack). Discard
the red plastic tray.
7. Place the Cathode Stack,
Top over the pre-soaked
Filter paper with the
electrode side facing up
and aligned to the right
edge. Remove air bub-
bles using the Blotting
Roller.
8. Place the Disposable
Sponge with the metal
contact on the upper
right corner of the lid.
Proceed to Performing
Blotting, below.
Performing Blotting
Perform blotting within 15 minutes of assembling the stacks with the gel.
disassembling the iBlot
®
Gel Transfer device
1. Open the lid of the iBlot
®
Device.
2. Remove the iBlot
®
E-PAGE
™
Tab (used for blotting E-PAGE
™
gels only). Rinse the tab with deionized water and store in a dry
place for future use.
3. Discard the iBlot
®
Disposable Sponge and iBlot
®
Cathode Stack, Top.
4. Carefully remove and discard the gel and filter paper. Remove the transfer membrane from the stack and proceed with the
blocking procedure or stain the membrane.
5. Discard the iBlot
®
Anode stack, Bottom.
6. At this point, the iBlot
®
Gel Transfer Device is ready for another run (no cooling period is required). If you are not using the
device, turn off the power switch.
downloading upgrades
To download iBlot
®
Device firmware upgrades, go to www.invitrogen.com/iblot. Follow instructions on the page to download
the upgrades.
Limited use Label License
This product is covered by Limited Use Label License 358: For Research Use Only: The purchase of this product conveys to the purchaser the
limited, non-transferable right to use the purchased amount of the product only to perform internal research for the sole benefit of the purchaser.
No right to resell this product or any of its components is conveyed expressly, by implication, or by estoppel. This product is for internal
research purposes only and is not for use in commercial applications of any kind, including, without limitation, quality control and commercial
services such as reporting the results of purchaser’s activities for a fee or other form of consideration. For information on obtaining additional
rights, please contact [email protected] or Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad, California 92008.
