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Leica VT1000 S – Vibrating-blade microtome

3.

Instrument Characteristics

3.2

Overview - VT1000 S

Buffer tray S

Emergency stop switch
(not visible here)

Fixture for
magnifier

Fiber optics
(optional)

Magnifier

Cold light source
Leica CLS 100 (optional)

Knife holder

Control panel

Magnifier
carrier

Cutting head

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Summary of Contents for VT1000S

Page 1: ...Operating Manual Leica VT1000S V1 8 English 12 2009 Always keep this manual with the instrument Readcarefullybeforeworkingwiththeinstrument HLFD 97 6 Microtome with Vibrating Blade ...

Page 2: ......

Page 3: ...ial number and year of man ufacture please refer to the name plate at the back of the instrument Leica Biosystems Nussloch GmbH The information numerical data notes and value judgments contained in this manual represent the current state of scientific knowledge and state of the art technology as we understand it follow ing thorough investigation in this field We are under no obligation to update t...

Page 4: ... the amplitude 20 5 5 Working with the VT1000S on a daily base 21 5 6 Daily routine maintenance and switching off the VT1000S 24 6 Malfunctions Meanings and Troubleshooting 25 7 Cleaning and Maintenance 30 7 1 Cleaning the instrument 30 7 2 Changing the fuse 31 8 Ordering Information Spare Parts Accessories Consumables 32 8 1 Additional accessories for standard size specimens functional descriptio...

Page 5: ... Information When making inquiries please specify correctly Instrument type Serial number Qualification of personnel The Leica VT1000S should be operated by trained laboratory personnel only All laboratory personnel designated to operate the Leica instrument must read this Operating Manual carefully and must be familiar with all technical features of the instrument before at tempting to operate it...

Page 6: ...ts This instrument has been built and tested in ac cordance with the safety requirements for elec trical equipment for measurement control and laboratory use To maintain this condition and ensure safe oper ation the user must observe all notes and warn ings contained in this Operating Manual For current information about applicable stan dards please refer to the CE Declaration of Con formity on ou...

Page 7: ... cautiously Never touch the cutting edge of knives and blades Do not leave knives blades and bladed knife hold ers unprotected All appropriate safety precautions must be met to avoid the risk of infection Protectiveclothesaccordingtosafetyregulations Working with harmful substances Safety mask gloves protective clothing must be worn Cover the magnifier during work breaks as it may act as a burning...

Page 8: ...h knife holder L 70 x 40 mm Specimen orientation 330 Section thickness selection 1 999 µm in 1 µm steps Magnifier assy Standard accessory 2 x Ambient conditions Operating temperature range min 5 C to max 40 C Relative humidity max 80 Altitude max 2 000 m above sea level Electrical data Nominal voltage range 10 100 V 240 V Rated frequency 10 50 60 Hz Power draw 50 VA Mains fuse T 1 25 A Pollution d...

Page 9: ...rument Characteristics 3 2 Overview VT1000S Buffer tray S Emergency stop switch not visible here Fixture for magnifier Fiber optics optional Magnifier Cold light source Leica CLS 100 optional Knife holder Control panel Magnifier carrier Cutting head ...

Page 10: ... limit positions Pause button stops sectioning process Control knob for sectioning speed Indication of selected section thickness or totalized section thickness in µm Selection button for sectioning thickness 1 999 µm selectable retraction and or volume Button for setting the limit stops of the sectioning window Start button for single continuous sectioning stroke Emergency stop button Fixing devi...

Page 11: ...l 14 0472 80011 VT1000S complete configuration 14 0472 35613 VT1000S Basic instrument 14 0472 35612 3 Specimen discs S non orientable 14 0463 27404 Buffer tray S 14 0462 30132 5 Countersunk screw M 5x8 14 2101 77121 2 Hose clamps 14 0481 41952 Knife holder S for injector and razor blades 14 0462 30131 1 Hexagon key w handle size 3 metric 14 0194 04764 1 Bottle of Cyanoacrylate adhesive 14 0371 274...

Page 12: ...they are complete 1 2 3 4 Fig 12 1 Fig 12 2 3 Lift the instrument out of the transport carton by the carrying straps 5 5 5 5 5 and place it on a suitable stable laboratory table The instrument is securely fastened to the baseplate with a screw 6 Tilt the instrument including the baseplate Fig 12 4 hold the instrument with one hand on the recess 8 for the buffer tray NEVER lift or hold it by the cu...

Page 13: ...3 2 to the bottom of the instrument 1 Ensure that the loose end of the drain tube is closed tightly with the matching stopper Secure the loose end of the drain tube in the holder at the rear of the instrument 2 1 4 Assembling the magnifier support and footswitch optional Fig 13 3 The magnifier support 3 is packaged separately Set it on the instrument as shown in Fig 13 3 Attach the optional footsw...

Page 14: ...ily accessible Power supply at a distance no greater than the length of the power ca ble 3m an extension cable must not be used Level installation location Substrate as free of vibration as possible Relative humidity should not exceed 80 Room temperature consistently between 5 C and 40 C Avoid vibrations direct sunlight and large temperature fluctuations The instrument MUST be connected to a groun...

Page 15: ...er cable that is intended for the local power supply 2 Make sure the mains cable is connected correctly to the instrument 3 Attach the magnifier carrier 4 Insert the buffer tray 5 Insert the knife holder 6 Insert a blade into the knife holder 7 Connect the magnifier support with optional fiber optic illumination as shown in Fig 13 3 insert plug 1 of the fiber optics light guide into socket 2 at th...

Page 16: ... 50 10 Speed rotating potentiometer with scale Function Continuous knife feed adjustment from 0 05 2 5 mm s Knifereturnstrokeisperformedatconstantspeed of 5 mm s The additional locking lever lever in 12 o clock position prevents the speed setting from being accidentally changed while sectioning is in progress SPEED Control knob with scale from 0 to 10 Function Continuous adjustment of knife sectio...

Page 17: ...fe edge past the specimen block and press once more to set the second sectioning window limit Press START to deactivate V Max The knife edge moves back to the first sectioning window limit and resumes sectioning at the previously selected speed 10 speed rotary potentiometer Function Start single or continuous sectioning stroke according to whether SINGLE or CONT mode has previously been selected s...

Page 18: ...position The REV FORW switch can also be used to stop a sectioning stroke which has been activated by pressing the START STOP button LED indication with adjusting button DISP and CLR function keys Function of LED indication Indicates the selected sectioning thickness or totalized section thickness Function of the button Selection of section thickness in 1 µm steps from 0 to 999 µm The specimen fee...

Page 19: ... While the knife is in motion the UP DOWN toggle switch is inoperational For the DOWN motion the toggle switch can be locked in the DOWN position for the UP motion the switch must be pressed and held in the UP position Whenthelowestpossiblepositionisreachedwiththetoggleswitchbeing locked in DOWN there will be both an audible and a visible signal Once the switch is unlocked the buffer tray is autom...

Page 20: ...the amplitude To obtain excellent sectioning results the amplitude requires adjustment according to the specimen type being sectioned To this end With a 2 5 mm Allen key loosen the clamping screw 1 and secure the eccentric on the bottom with your finger Selectable amplitude positions are from left to right 0 2 mm 0 4 mm 0 6 mm 0 8 mm 1 mm Slide the amplitude clamping screw to the desired amplitude...

Page 21: ...esignalstops If necessary fill crushed ice into the cooling bath 3 Fill the buffer tray 1 with cooled buffer solution Fix the specimen onto the specimen disc with cyanoacrylate adhesive 21 2 Insert the specimen disc 5 with the specimen into the buffer tray using the manipulator 6 Use the manipulator 8 to rotate the specimen disc into the desired po sition Tighten with a 3 mm Allen key 7 The clampi...

Page 22: ...the clear ance angle every time you change the knife Make an adjustment only if required by an application for technical reasons e g differ ent type of tissue To insert the blade loosen the clamping screw 3 located on the knife holder Clean the blade Insert the blade into the knife holder 4 Secure the blade with clamping screw 5 The blade must fit tightly against the entire length of the inner lim...

Page 23: ...ECTIONING WINDOW button Switch the SINGLE CONT button to CONT Push the START STOP button The instrument will now trim the specimen at the selected trimming thick ness until you push the START STOP button once more Once you have reached the desired specimen level for sectioning use the button to select the desired thickness for sectioning For sectioning proceed as follows Select the desired section...

Page 24: ...specimen plate and lay it flat on the stage Remove the specimen using a single edge blade Then remove remains of cyanoacrylate adhesive from the specimen disc Remove and empty out the buffer tray Dispose of the contents of the buffer tray properly Drain the cooling bath To do so release the tube from its holder at the rear of the instrument and dispose of the contents of the ice bath into a suitab...

Page 25: ...rror Troubleshooting Collision of knife and specimen holder Audible warning signal Return stroke is not completed Audible warning signal Return stroke is not completed Clearance angle adjustment If a clearance angle wider than 5 is selected specimen disc and knife edge can potentially collide with each other When working with orientable specimen holders knife edge and specimen holder can col lide ...

Page 26: ... locking function of the UP DOWN button With the UP DOWN button locked in the DOWN position the instrument was switched off via the main switch at the rear of the instrument switched on again without releasing the UP DOWN button to its center position With the UP DOWN button locked the instrument was switched off via the emergency stop foot switch or Emergency stop button and after that the emerge...

Page 27: ...s Sources of error Troubleshooting Button s jammed or defective Locking function REV of REV FORW button defective Error on the UP DOWN button DOWN locking function Feed mechanism defective Important electronic compo nent defective Feed motor defective Light barrier error forward feed Light barrier error section thickness feed Push the button several times to unlock have defective but ton replaced ...

Page 28: ...l has been reached height adjustment of specimen via buffer tray User has tried to select a spec imen thickness via the but ton which is below the mini mum value 0 µm or above the maximum 999 µm Switch the instrument off call the Technical Service Instrument can still be used though there will be certain limitations all values will be set to default values New values sectioning win dow feed loweri...

Page 29: ...Do not use the instrument when in this condition The visible clamping screws have become loose during sectioning These symptoms may occur from time to time and are un avoidable as the clamping screws which have to be op erated by the user cannot be sealed 6 Malfunctions Meanings and Troubleshooting Error messages symptoms Sources of error Troubleshooting Audible warning signal When operating the i...

Page 30: ...ove the buffer tray empty it and rinse it separately with water see also page 23 7 1 Cleaning the instrument Always remove the knife blade before detaching the knife holder from the instrument Always put the knife blade back into the knife case or blade dispenser when not in use When using cleaning agents observe the manufacturer s safety instructions and the laborato ry regulations valid in the c...

Page 31: ...ower supply at the power socket Then check the fuses at the rear side of the in strument To do so proceed as follows Using a screwdriver 13 carefully push out the fuse insert 14 Fig 31 Remove the fuse insert it contains two fuses 15 Check that the thin wire 16 in the glass cap illary of a fuse is intact If not replace the fuse the standard scope of delivery includes two replacement fuses Before pl...

Page 32: ...4 0463 46424 Specimen plate L non directional 14 0463 27405 Knife holder S for max 20mm high specimens 14 0462 31950 Knife holder L for specimens 20mm high 14 0462 31949 Injector blades 1 dispenser with 20 blades 14 0358 27411 Sapphire knife 14 0216 39372 Cyanoacrylate glue 14 0371 27414 The orientable specimen discs S can be rotated around their center as well as tilted on one axis The non orient...

Page 33: ...330 Ref no 14 0463 27406 8 2 Additional accessories for large specimens functional description Fig 33 3 shows a configuration for large specimens consisting of knife holder L buffer tray L and specimen disc L not orientable Ref no 14 0463 27402 Knife holder L Ref no 14 0463 27408 Buffer tray L Ref no 14 0463 27405 Specimen disc L non orientable Fig 33 1 1 2 Fig 33 2 8 3 Foot switch functional desc...

Page 34: ...ons BEFORE working with the specimens see the Short Instruc tion Manual for the minichiller Ref no 14 0463 46424 The double walled buffer trays can be equipped with clamps that hold the hose provided in the correct position for gassing the buffer First connect the hoses provided with the min ichiller to the rear side of the cooling unit see the Short Instruction Manual then connect the oth er end ...

Page 35: ...ter the magnifier has been mounted into the fixture Then connect the fiber optics to the cold light source Ref no 14 0502 30028 Magnifier To be inserted into the fixture Ref no 14 0462 31191 Leica CLS 100 cold light source Serves as a light source for the fiber optic illumination 100 120 V 50 60 Hz Ref no 14 0502 30214 230 V 50 60 Hz Ref no 14 0502 30215 240 V 50 60 Hz Ref no 14 0502 30216 Fig 35 ...

Page 36: ...our Leica sales organization or the organiza tion from which you have purchased the contractual product shall apply exclusively Service information If you are in need of technical customer support or spare parts please con tact your Leica representative or the Leica dealer where you purchased the instrument Please provide the following information Model name and serial number of the instrument Loc...

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