23
Leica VT1000 S – Vibrating-blade microtome
•
Fix the knife holder
(1)
with the knife holder clamping screw
(2)
.
•
Use the
REV/FORW
-rocker button to place the knife edge right behind
the rear edge (from user's view) of the specimen.
•
Pull the
UP/DOWN
-rocker button into the UP-direction and keep it in the
UP position until the specimen surface is shortly below the level of the
knife edge (see arrow
(3)
).
•
Select sectioning speed and sectioning frequency with the control knobs
SPEED
and
FREQ
.
•
Use the
+/-
button to select a sectioning thickness for trimming.
•
Select a sectioning range appropriate to the size of the specimen with
the
SECTIONING WINDOW
-button.
•
Switch the
SINGLE/CONT
-button to
CONT
.
Push the
START/STOP
-button.
The instrument will now trim the specimen at the selected trimming thick-
ness until you push the
START/STOP
button once more.
•
Once you have reached the desired specimen level for sectioning, use
the
+/-
button to select the desired thickness for sectioning.
For sectioning proceed as follows:
•
Select the desired section thickness via the
+/-
button.
•
Switch the
SINGLE/CONT
T
T
T
T
-button to
SINGLE
.
•
Push the
START/STOP
-button.
The instrument will now produce a section
(4)
. When the section is fin-
ished, the knife will automatically stop at the rear end position behind
the specimen (from the user´s view).
•
Pick up the section as shown on the left using a brush
(5)
to mount it on a
glass slide
(6)
.
5.
Operation
Fig. 23.1
Fig. 23.2
1
2
3
4
5
6
Fig. 23.3
Summary of Contents for VT1000S
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