Leica TCS SP8 SMD, User Manual

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F(L)CS Data Acquisition
8. Select the next measuring point, click
Add
, and create another snapshot as necessary.
9. Continue until you have marked all the points you want. The maximum number of points
is 200.
10. Different measuring points in an xyz or xzy stack can be located on different z or y planes,
respectively. Select the z or y planes you want in the image display. Place the crosshair
on the xy point you want in the desired z plane or xz point on a certain y position.
11. Then click
Add
(
Figure 179
).
12. After this, a position list is created that can be saved by selecting Save list and called up
at a later time (
Figure 179
). Individual points can be deleted from the list with
Remove
,
and the entire list can be deleted with Remove all ( Figure 179 ).
13. Enter a measurement time in the LAS AF software, that is, the time required for a single
measurement ( Figure 179 ).
14. The selected measurement duration and the instrument parameter setting for the FCS
measurement are applied to all individual measuring points.
15. If you are working in xyz mode, you can still change the zoom within the
Measurements
step.
18.4.3 FCS Time Series at Multiple Measuring Points
In the
Setup
tab, it is possible to define a time series in two ways:
• Repeated measurements at single points (definition in the
Definition of Point
Measurement field)
• Repeated processing of all points (definition in the
Time Series
field).
By combining both kinds of definition, a time series can be created within a time series.
For repeated measurement at individual points, the following functions are available:
• Pre-bleach : Enables you to define a time in which the laser is switched on before the
measurement begins. This feature can be used to pre-bleach the specimen before the
measurement (
Figure 181, item 1 ).
• Measurement duration : Specifies the duration of an individual FCS measurement
( Figure 181, item 2 ). The minimum measurement duration is 1 second; the maximum
measurement duration is 10 minutes.
• Repetition at point : The entered number specifies how often the measurement at this
point is repeated before the system moves on to the next point (
Figure 181, item 3
). The
maximum number of repetitions is 1000.
• Repetition interval : Specifies the time between the beginning of one measurement and
the following measurement at this point. The value cannot be smaller than the sum of
measurement and pre-bleach time (
Figure 181, item 4
).
• Minimize : If the Minimize box is checked, repetitions will be carried out as fast as
possible (
Figure 181, item 5
).
Do not leave the Measurements step until FCS data acquisition
You may no longer leave the
Measurements
step until the FCS data
acquisition, otherwise the position list becomes invalid.