Leica TCS SP8 SMD, User Manual

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F(L)CS Data Acquisition
Figure 172: Controlling the FCS filter wheel
Due to the configuration of the FCS filter wheel, the following laser lines are not available
for FCS and are therefore grayed out in the software (
Figure 172 ):
• 476 nm
• 496 nm
The following combinations are also not possible (although the individual lines can be used
on their own):
• 458 nm with 488 nm
• 488 nm with 514 nm
The combination of 458 with 514 nm can be used, however.
The FCS filter wheel is used only in the Setup FCS and Measurements steps of the FCS
Wizard. In all other cases it is in an empty position. This means that all argon laser lines used
in the FCS measurement (
Setup FCS and Measurements steps in the FCS Wizard) are
attenuated for the image acquisition relative to the normal mode: The 458-nm line is
attenuated by 25 percent, the 488-nm line by 75 percent, the 514-nm line by 75 percent, and
the combination of 458 and 514-nm lines by 50 percent. The other lasers are not affected by
attenuation.
18.3.2.2 Using Pulsed VIS Diode Lasers
For FCS, the pulsed diode lasers can be used in the VIS range. The observation volume and
therefore the diffusion time are greater than when using the continuous wave lasers.
For what comes next, follow the instructions in Chapter 18.2.2 .