Leica TCS SP8 SMD, User Manual

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F(L)CS Data Acquisition
has no effect on the detector; it modifies the color look-up table, which renders low-
intensity structures more visible. It may also be helpful to accumulate over several
images. The
Offset
setting on the control panel has no effect on the image.
Figure 168: Acquiring the reference image using APDs, setting the X1 port
7. Optimize the image quality. The functions for this are the same as outside the wizard.
8. Acquire an image by clicking the Capture image acquisition button or acquire an xyz or
xzy stack by clicking the
Start
image acquisition button. The start and finish of the stack
are defined interactively, in the usual way. The stack image is automatically added to
your LAS AF experiment.
18.2.2 Selecting Laser Lines as an Excitation Source for the Image
Acquisition
All available lasers can be used for the image acquisition ( Figure 169 ). You will find a
description of the mode of operation in Chapter 15 .
18.2.2.1 Using Continuous Wave Lasers
For capturing the emitted fluorescence, set the acousto-optical beam splitter to
fluorescence (
Figure 169
). Choose a suitable laser line and adjust the intensity to the desired
level using the AOTF slider. If the system has a multifunction port (
MFP
), it should be set to
Substrate .
Dependency of the spectral detection range of the APDs
The spectral detection range of the APDs is determined by the SMD filter
cube used, not by the slider settings for the photomultipliers.