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154
FLIM Data Acquisition
6. Define the first and last image of the stack and the number of planes via the stack dialog
(
z-stack
or
y-stack
) (
Figure 139:
FLIM stack definition xzy
7. Define the duration of the individual FLIM images as described in
. The
option
Acquire until max ___ photons/pixel
is of particular interest for acquiring FLIM
stacks. It enables corrections due to intensity changes caused by different sample
penetration depths.
8. Start the FLIM stack acquisition with the image acquisition button
Run FLIM
.
In LAS AF, you obtain a stack of averaged images and, in SymPhoTime, a series of FLIM
images.
16.3.5
Defining an FLIM Time Series
To acquire an FLIM stack, proceed as follows:
1. Select the specimen position.
2. Select either
Acquisition Mode xyt
or
xzt
as the scan mode on the
Acquisition
tab.
Optimize the instrument parameter setting for FLIM measurement.
3. Define a file name in the
Setup
tab (
).
4. Open the
Acquisition
tab.
Summary of Contents for TCS SP8 SMD
Page 1: ...10 Living up to Life User Manual Leica TCS SP8 SMD for FCS FLIM and FLCS ...
Page 4: ...4 Copyright ...
Page 14: ...14 Contents ...
Page 18: ...18 Intended Use ...
Page 20: ...20 Liability and Warranty ...
Page 28: ...28 General Safety Notes ...
Page 32: ...32 Additional Notes on Handling the System ...
Page 44: ...44 System Overview and Properties ...
Page 60: ...60 SMD Components Figure 31 DSN 102 Dual SPAD Power Supply ...
Page 80: ...80 Safety Features ...
Page 102: ...102 Switching On the System ...
Page 116: ...116 LAS AF ...
Page 214: ...214 Changing the Specimen ...
Page 216: ...216 Changing the Objective ...
Page 238: ...238 Switching Off the System ...
Page 242: ...242 Repairs and Service Work ...
Page 244: ...244 Maintenance ...
Page 246: ...246 Disassembly and Transport ...
Page 248: ...248 Disposal ...
Page 254: ...254 Contact ...
Page 256: ...256 Recommended Literature ...
Page 266: ...266 Appendix Figure 225 Declaration of conformity ...
Page 268: ...268 Appendix ...
Page 269: ......