Leica TCS SP8 SMD, User Manual

Page: 150 / 270

150
FLIM Data Acquisition
You can also load FLIM instrument parameter settings in the
Setup Imaging
step or outside
the FLIM Wizard. In this case, these settings are treated as settings for normal image
capture intensities.
16.3 Measurements Step – Time Series for FLIM Measurement
at Multiple Points
After optimizing the FLIM measurement conditions, it is possible to have FLIM
measurements run automatically. To do so, go to the
Measurements step.
16.3.1 FLIM Network Connection
The LAS AF and SymPhoTime programs are synchronized via a network connection. Each
FLIM measurement started in LAS AF in the
Measurements
step of the FLIM Wizard
generates a new data file in SymPhoTime.
In addition to the synchronization, the network also enables the automatic transmission and
implementation of scan parameters (format, scan speed, unidirectional or bidirectional
scanning). In addition, other relevant information, such as the file name, instrument
parameter setting (IPS) or comments entered by the user in LAS AF are transferred and
stored in SymPhoTime.
During the FLIM measurement, an online FLIM image is displayed that is automatically
saved along with the raw data in SymPhoTime. After the data acquisition, a more detailed
data analysis can then be carried out offline.
16.3.2 Definition of the FLIM Measurement File Names Transferred to
SymPhoTime
1. Select the
Setup
tab.
2. Enter the name of the FLIM data file used in SymPhoTime in the Base name field in the
pull-down menu
File/Series Definitions
(
Figure 136
).
Observe the SymPhoTime manual
For more detailed information about data analysis, refer to the
SymPhoTime manual.