Leica SP5 confocal, User Manual

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Acquiring Images at different stage positions (Mark and Find)
1. Enable the Mark and Find option in the Acquisition Mode
window. The mark and find window will appear.
2. Here you can see an image of the stages travel range. The cross hairs point to a
small square box which represents your current XY position. Click on the +
magnifying glass to zoom in on the box.
3. Clicking the mouse anywhere within the scaled region will cause the stage to
move to that position, USE WITH CAUTION. Only click small distances from the
current position (indicated by cross hair).
4. To mark a field of view to be captured click the Mark Position button.
5. Continue to add further positions to be scanned by using the joystick to move
around. The positions are added to a dropdown list, selecting a position from the
list will move the stage to that position.
6. The Show All Positions button will highlight within the stage area the fields of
view you have marked for scanning.
7. You can delete a single position using the Clear Position button or delete all
using the rubbish bin button.
8. If acquiring a Z stack at each position you can either use the same stack
dimensions as defined for one position at all positions or define individual Z
stacks per position. This is set using the Same Stack For All tick box.
Acquiring a Time-­‐Lapse
1. Select a beam path setting and configure each wavelength channel as required.
Follow the sections above to add a tile scan, mark and find etc.
2. In the Acquisition Mode window choose the relevant time
experiment from the dropdown list xt, xyt, xyzt etc.
3. This will open the Time window. Define the total
duration of the time lapse and the frequency of
captures in the Interval box.
4. The minimise tick box will set the shortest possible
delay based on your scan speed etc.
5.
The Stacks option allows you to choose how many time
points you collect instead of having to input a du
interval.