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5

Leica RM2125

Notes, i.e. important user information
appear in a gray box and are marked
by an information symbol .

Dangers, warnings and cautions ap-
pear in a grey box and are marked by
a warning triangle .

1.1 Symbols in the text and their meaning

1.

Important notes

(5)

1.3 Designated use

The Leica RM2125 / Leica RM2125 RT are manu-
ally operated rotary microtomes, used to pro-
duce thin sections of specimens of different
hardness in both routine and research applica-
tions in biology, medicine and industry.

They are designed to section soft materials em-
bedded in paraffin as well as harder specimens,
provided those specimens are still suitable for
manual sectioning.

Any other use of the instrument is considered

improper!

1.4 Instrument type

All information provided in this manual applies
only to the instrument type indicated on the cov-
er page.

A name plate indicating the instrument serial
number is attached to the back of the instru-
ment.

Fig. 1

1.2 Qualification of personnel

•

The Leica RM 2125 may be operated only by
trained laboratory personnel.

•

All laboratory personnel designated to oper-
ate the Leica RM2125 must read this instruc-
tion manual carefully and must be familiar
with all technical features of the instrument
before attempting to operate the Leica
RM2125.

Figures in brackets refer to item num-
bers in drawings.

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Summary of Contents for RM2125

Page 1: ...a RM2125 Leica RM2125RT Rotary microtome Instruction manual Leica RM2125 RM2125RT V2 3 English 02 2009 Always keep this manual together with the instrument Read carefully before working with the instrument ...

Page 2: ......

Page 3: ... the present manual are not to be consid ered as warranted characteristics of our products These are determined only by the contract pro visions agreed between ourselves and our cus tomers Leica reserves the right to change technical specifications as well as manufacturing pro cesses without prior notice Only in this way is it possible to continuously improve the technolo gy and manufacturing tech...

Page 4: ...g the knife holder 17 5 Operation 18 5 1 Clamping the specimen 18 5 2 Inserting the knife disposable blade 18 5 3 Adjusting the clearance angle 19 5 4 Orienting the specimen possible only with orienting fixture for specimen clamps 20 5 5 Trimming the specimen 21 5 6 Sectioning 23 5 7 Changing specimen 23 5 8 Accessories 24 5 8 1 Standard specimen clamp 24 5 8 2 Vee insert 24 5 8 3 Foil clamp type ...

Page 5: ...affin as well as harder specimens provided those specimens are still suitable for manual sectioning Any other use of the instrument is considered improper 1 4 Instrument type All information provided in this manual applies only to the instrument type indicated on the cov er page A name plate indicating the instrument serial number is attached to the back of the instru ment Fig 1 1 2 Qualification ...

Page 6: ...nery directive 98 37 EC and the safety regulations for laborato ry devices as specified below The latest information on the standards applied can be found in the CE declaration of conformity on the Internet under www histo solutions com The protective devices on both instrument and accessories may neither be removed nor modi fied Only authorized and qualified service personnel may repair the instr...

Page 7: ...e very careful when handling microtome knives or blades The knife or blade edges are ex tremely sharp and can cause severe injuries Always remove the knife blade before detaching the knife holder from the instrument Knives that are not in use should always be stored in the knife case Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife Always cla...

Page 8: ...ing mechanism The handwheel 15 can be locked in the 12 o clock position Fig 2 When pushing the handwheel handle 1 in wards to the left the handwheel will lock in place when next reaching the 12 o clock posi tion Function check To activate the locking mechanism push the handle 1 to the left The handwheel locks in place in the 12 o clock position and cannot be rotated any further To unlock the handw...

Page 9: ...ny manipulation of knife or specimen prior to changing the specimen block and during all work breaks 2 Safety Knife holder N The knife guard 3 of the knife holder N can be easily positioned via the two buttons 4 Fig 3 To cover the knife edge push both cover strips of the knife guard to the center Knife holder E The knife guard of the knife holder E consists of a hinged shackle 5 To cover the edge ...

Page 10: ...base Smooth turning handwheel Lever for activating the handwheel brake Handwheel handle with locking mechanism Lever for activating the mechanical trimming function Orientable fixture for specimen clamps Coarse feed wheel Leica RM2125 RT Fig 5 Fig 6 Knife holder E Knife holder N Knife holder base with lateral displacement feature Knife holder base without lateral displacement feature Clamping leve...

Page 11: ...ousing Section thickness is selected via a turning control knob and indicated in the small window integrated into the instrument housing Section thickness settings from 0 5 to 60 μm can be selected Both models are equipped with a slot cover preventing section waste from entering the interior of the instrument Both the Leica RM2125 and Leica RM2125 RT are available in two ver sions 1 Leica RM2125 R...

Page 12: ... Total horizontal specimen feed 25 mm Vertical specimen stroke 59 mm Specimen retraction RM2125 RT only 220 μm Dimensions and weight Width 400 mm Depth 470 mm Height 295 mm Working height knife edge 105 mm Weight without accessories 29 kg Optional features and optional accessories Specimen orientation optional horizontal 8 vertical 8 Rotability 90 Trimming thickness RM2125 RT only 10 μm 50 μm Knif...

Page 13: ...4 0212 04091 1 Instructions manual language CD 14 0498 80001 The above accessories plus any further accessories you ordered you will find in the cardboard box packed on top of the instrument see Fig 7 Carefully check the delivery against the packing list delivery note and your order Should there be any discrepancy please contact the Leica sales unit handling your order or your Leica dealer 4 1 Ins...

Page 14: ...usting knob Place wood pallet with instrument onto a stable bench Grasp the front edge of the pallet and lift it slightly Use size 8 Allen key 7 to remove the secur ing bolt 6 and washer located underneath the pallet Cut open plastic cover 5 and remove Grasp front and rear edge of instrument base plate and lift instrument from pallet Place instrument on stable laboratory bench Two sliders 8 attach...

Page 15: ...apter 5 8 Ac cessories fit into the fixture To fasten a specimen clamp in the fixture pro ceed as follows With handwheel 15 move fixture 10 to up permost position and activate the handwheel locking mechanism Release clamping lever 11 rotate counter clockwise Slide the dovetailed tenon 13 of the speci men clamp from the left into the dovetail mortise of the clamping device 14 of the fix ture To sec...

Page 16: ...n clamp 12 onto fixture for specimen clamps see Fig 9 3 and fasten with two screws 17 Remove the two screws 17 use Allen key size 3 in order to detach the dovetail tenon 13 from the specimen clamp 12 Fig 9 2 Move fixture 10 to uppermost position turning the handwheel and activate the handwheel locking mechanism Remove dovetail clamping device 14 from the fixture Fig 9 1 To do so loosen the four sc...

Page 17: ... plate 23 Rotate clamping lever 20 clockwise to se cure the knife holder base on the microtome base plate position secured Rotate lever 24 counterclockwise to re lease Slide groove of knife holder 25 onto T piece 26 of knife holder base 21 Rotate lever 24 clockwise to clamp Fig 10 4 6 Mounting the knife holder base Fig 11 4 7 Mounting the knife holder 19 20 21 26 25 22 23 24 21 ...

Page 18: ...the handle of the handwheel in the 12 o clock position Insert a specimen block into the specimen clamp See chapter 5 8 Accessories for details on how to insert the specimen block into the different types of specimen clamps 5 2 Inserting the knife disposable blade Be very careful when handling microtome knives or blades The knife or blade edges are extremely sharp and can cause severe in juries Car...

Page 19: ...ate lever 24 counterclockwise to re lease Move the knife holder until the index mark of the desired clearance angle coincides with the reference line on the knife holder base Example Enlarged detail showing a clearance angle setting of 5 For knife holder E we recommend clearance angle settings between 1 and 3 Hold the knife holder in the selected position and rotate lever 24 clockwise to clamp 5 3...

Page 20: ...prior to the next section the block will advance by the retraction value PLUS the selected section thickness This may cause damage to both specimen and knife 5 4 Orienting the specimen possible only with orienting fixture for specimen clamps When working with the large size standard specimen clamp 50 x 55 mm the north south orientation can only be utilized up to an angle of about 4 instead of 8 Fi...

Page 21: ...ront limit posi tion the specimen feed mechanism stops working 5 5 Trimming the specimen 37 32 Fig 14 Fig 15 33 34 15 5 Operation Trimming the specimen with the coarse feed Unlock the handwheel push handle 1 inward or release brake mechanism via lever 2 Turn coarse feed wheel 31 to bring the specimen gradually closer to the knife At the same time turn handwheel 15 to trim the specimen down to the ...

Page 22: ...ces by 10 μm or 50 μm respectively When the lever is released trimming is deac tivated lever flips back to zero position The selected section thickness is not added to the trimming value If however the section thickness se lected is greater than the trimming value the specimen will advance by the section thickness without the trimming value being added Turn the coarse feed wheel to bring the speci...

Page 23: ...cimen clamp backwards far enough to be able to start trimming the new specimen block For that purpose if you work with a knife holder with lateral adjust ment option move the knife holder base knife holder to the desired position see chap 5 8 7 or if your knife holder base is without lateral adjustment move the knife or blade itself Start sectioning by rotating the handwheel 15 evenly in clockwise...

Page 24: ...ise the movable jaw until the block is securely clamped 5 8 Accessories 5 8 2 Vee insert The vee insert 44 fits into the movable jaw of the standard specimen clamp for clamping round specimens Turn knurled screw 40 counterclockwise lowering the movable jaw 41 as far as it will go Insert pin 43 of vee insert 44 into the hole 45 of the movable jaw 41 Insert the specimen Turn knurled screw 40 clockwi...

Page 25: ...both for clamping very small foil pieces and flat angular samples It is mounted in the standard specimen clamp Clamping flat specimens To clamp flat samples remove the longer set screw 51 and insert the shorter one 52 Use Allen key size 4 49 to unscrew longer setscrew 51 to the left Insert shorter setscrew 52 into the thread Insert specimen 53 between movable jaw 48 and fixed jaw 47 To clamp the s...

Page 26: ... 58 on the back of the foil clamp is pointing to the right or to the left Rotate knurled screw 40 clockwise until the foil clamp is securely clamped 5 8 4 Foil clamp type 2 All common brands of cassettes fit into the universal cassette clamp UCC either horizontally or vertically Pull lever 60 forward Insert cassette 61 horizontally or vertically Release the lever to secure the cassette in position...

Page 27: ...e rotating the clamping ring 66 clockwise To orient the specimen insert pin 67 into hole 68 and turn counterclockwise to re lease the clamping mechanism The speci men can now be oriented as desired Turn pin 67 clockwise to secure the speci men 5 8 6 Holder for round specimens The holder for round specimens accomodates cylindrical samples Inserts for cylindrical specimens of 6 15 and 25 mm diameter...

Page 28: ...e lever 20 clockwise Fig 26 5 8 7 Knife holder base Fig 25 Knife holder base without lateral displacement 21 1 Knife holder base with lat eral displacement 21 2 Knife holder base with lateral displacement The knife holder base with lateral displacement feature 21 2 consists of two pieces and can be adjusted in north south as well as in east west direction East west adjustment The lateral displacem...

Page 29: ...s 73 on the right and left of the knife holder forward lowering the knife support bar to the lowest possible posi tion thus ensuring that the knife edge will not be damaged when inserting the knife Unscrew the clamping screws 75 as far out as possible turn counterclockwise Hold the knife 74 at the knife back and carefully insert it from one with the cutting edge facing upwards Knife height adjustm...

Page 30: ...ventional disposable knives It is available in two versions with rear pressure plate 80 for low profile blades 77 or for high profile blades 78 Both rear pressure plates are also available separately and one type can easily be exchanged for the other Changing the rear pressure plate 80 With an Allen key size 4 loosen the four screws 82 at the rear of the knife holder and remove them Remove pressur...

Page 31: ...ate 0 1 0 2 mm 77 80 84 85 Insert pressure plate 80 and tighten screws 82 only slightly so that orienting the pres sure plate is still possible With setscrews 83 adjust the pressure plate so that the bearing surface 84 for the blade 77 is approx 0 1 0 2 mm higher than the surface of the lateral jaws of the knife holder 85 This is particularly important for instruments the knife holder base of whic...

Page 32: ...lder see drawing Insert a blade 77 and tighten the clamping mechanism 89 lightly The setscrews 87 on the underside of the knife holder accessible through openings in the underside of the knife holder enable you to height adjust the front pressure plate A size 2 Allen key is needed to do the adjustment With screw 88 adjust pressure plate 86 so that only the upper edge of the pressure plate actually...

Page 33: ...specimen clamp Foil clamp Type II Universal cassette clamp Holder for round specimens Vee insert for round specimens Knife holder base with lateral displacement feature Knife holder base without lateral displacement features Leica RM2125 basic instrument Fixture for specimen clamps orientable Fixture for specimen clamps non orientable Pressure plate exchangeable Standard cassette ...

Page 34: ...er 3 rgs silver 14 0502 38002 Clamping ring d 6 mm 14 0356 08322 Clamping ring d 15 mm 14 0356 09200 Clamping ring d 25 mm 14 0356 08320 Knifeholdbase non orientable silver 14 0502 37962 Knifeholdbase orientable silver 14 0502 37992 Knifehold E low profile silver 14 0502 37995 Pressure plate rear HP 22 14 0502 29553 Knifehold E high profile silver 14 0502 37996 Pressure plate rear LP 22 14 0502 29...

Page 35: ...ining acetone or xylene The varnished surfaces are not resistent to acetone or xylene No fluid must enter the interior of the instrument during cleaning Before each cleaning carry out the following preparatory steps Move specimen clamp to upper limit position and lock handwheel Remove blade or knife from knife holder and insert into the receptacle for used blades in the base of the blade dispenser...

Page 36: ...per edge of pressure plate 86 is parallel to and at the same height as the upper edge of the rear pres sure plate 80 sde Fig 30 page 31 If necessary adjust the pressure plate chapter 5 8 9 Cassette clamp Detach cassette clamp for a thorough cleaning removing all paraffin residues 12 Place cassette clamp 12 in a maximum 65 C hot oven and keep there until all paraffin has become liquefied and draine...

Page 37: ...the instrument daily Once a month lubricate the following com ponents with oil no 405 part of standard de livery 1 to 2 drops are sufficient The movable parts of the fixture for spec imen clamps 10 and of the dovetail mor tise 14 The T piece 19 on the microtome base plate Clamping levers 2 and 20 on the micro tome Guide rails 91 for the knife holder base on the microtome base plate Clamping levers...

Page 38: ...knife holder 7 2 Malfunction 1 No specimen feed i e no more sections 3 Streak present in sections with knife holder E 2 Sections compressed Sections are extremely com pressed wrinkled or jammed to gether Select sectioning speed and or trimming thickness according to specimen hardness Reduce section thickness turn handwheel at slower speed Move specimen backwards coarse feed wheel 2 Blades wear out...

Page 39: ...act concluded being regarded as binding only the warranty conditions of your local Leica sales unit or of the company from which you acquired the product Technical service information If you require technical service or replacement parts please contact your Leica sales office or dealer who sold the product Please provide the following information Model name and serial number of the instrument Loca...

Page 40: ...p to the date mentioned below The following harmonized standards were applied DIN EN ISO 12100 1 2003 Safety of machinery Basic concepts general principles for design Part 1 Basic terminology methodology DIN EN ISO 12100 2 2003 Safety of machinery Basic concepts general principles for design Part 2 Technical principles and specifications In addition the following in house standards were applied DI...

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