Leica M125 C User Manual Download Page 10

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Leica M Series User Manual

Eyepieces

Magnification Factors of the Eyepieces

Health Notes

Dioptric Correction

Dioptric Correction and Parfocality

Graticules 65

Photography & Video

Photography & Video

Photo Tubes and C-mounts

Trinocular Video/Phototube 50 %

Trinocular Video/Phototube 100 %

Trinocular Video/Phototube 100 %: ErgoTube 5°– 45°

Microscope Carrier

The Microscope Carrier AX

The Objective Nosepiece

Objectives and Optical Accessories

The Different Types of Objectives

Bases

Transmitted-light Base Leica TL BFDF: Controls

Transmitted-light Base Leica TL BFDF: Operation

Leica IsoPro (Non-motorized): Controls

System Illumination

Leica LED5000 MCI

Leica LED5000 RL

Accessories

Leica Hand Wheel and Foot Switch

Leica SmartTouch

Dimensional Drawings

Leica M125 C / Leica M165 C

Leica LED5000 MCI

Leica LED5000 RL

Leica LED5000 NVI

Leica MATS TPX

Specifications

Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A

Transmitted-light Base Leica TL BFDF

101

Motorized Focus Drives With "Zoom" Carrier
(M125 C, M165 C, M205 C, M205 A)

102

Appendix

Calculating the Total Magnification and Field of View Diameter

104

Care, Maintenance, Contact Persons

105

Contents (Continued)

Summary of Contents for M125 C

Page 1: ...Leica M125 C Leica M165 C Leica M205 C Leica M205 A User Manual...

Page 2: ...emicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abraded particles O O In most cases we can provide special...

Page 3: ...onal safety maintenance and accessories The Safety concept booklet contains addi tional safety information regarding the service work requirements and the handling of stereo microscope accessories and...

Page 4: ...indicates especially impor tant information that if not observed can cause the following O O Hazards to personnel O O Functional disturbances or damaged instruments Danger due to hot surface This symb...

Page 5: ...re outside of the scope of this manual Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims Place of use O O Refer to Safety Concept booklet O O...

Page 6: ...s O O Refer to Safety Concept booklet Disposal O O Refer to Safety Concept booklet Legal requirements O O Refer to Safety Concept booklet EC Declaration of Conformity O O Refer to Safety Concept bookl...

Page 7: ...her defective nor interrupted since otherwise a malfunction poses a risk of fatal injury Never unplug the power plug with moist or wet hands There is danger of an electric shock Instrument safety and...

Page 8: ...ug NEMA 5 15P or unscrew any mechanical components unless expressly instructed to do so in the instructions The microscope must be connected to a grounded outlet Always position the microscope so that...

Page 9: ...f the Focus Drive 55 Changing the Magnification Zoom 56 Ratchet Steps and Magnification Levels 57 Parfocality More Comfort and Convenience for Your Work 58 Iris Diaphragm 59 General Instructions 2 Saf...

Page 10: ...ansmitted light Base Leica TL BFDF Controls 79 Transmitted light Base Leica TL BFDF Operation 80 Leica IsoPro Non motorized Controls 81 System Illumination Leica LED5000 MCI 83 Leica LED5000 RL 85 Acc...

Page 11: ...design ensures that the M series adapts perfectly to your needs no matter which accessories you require for your tasks Though the reliability and robustness of Leica stereo microscopes is legendary li...

Page 12: ...en by the observer becomes distorted a sphere becomes an ellipse a flat surface curves toward the observer Limits are made to be broken The Leica M205 C is the world s first stereo microscope with a z...

Page 13: ...parameters such as the magnifica tion the illumination the position of the iris diaphragm and more to the Leica LAS X soft ware Thus you always know the conditions under which an image was acquired Fe...

Page 14: ...ything is Relative The Leica M series provides maximum flexibil ity in choosing equipment thanks primarily to the modular configuration and the compatibil ity that Leica has painstakingly maintained f...

Page 15: ...focus If you prefer you can continue to use the previ ous objective series In this case parfocality is no longer guaranteed Tubes The interface between the optics carrier and the tube has remained th...

Page 16: ...assembled and commissioned by your Leica consultant click here to skip through the instal lation instructions and go directly to the Quick Start Guide on page 49 If on the other hand you are assemblin...

Page 17: ...Leica M Series User Manual Assembly 17 Assembly...

Page 18: ...e corresponding base Tools used O O Hex socket screwdriver 3mm Assembling the column adapter 1 Securely install the column adapter on the column using the four included screws Assembling the focusing...

Page 19: ...ding base Tools used O O Hex socket screwdriver 3 mm Assembling the column adapter 1 Securely install the column adapter on the column using the four included screws Assembling the focusing column 2 S...

Page 20: ...ht base the focus ing column and motorized focus are installed directly on the base no extension plate is required Tools used O O Hex socket screwdriver 3 mm Assembly 1 Place the focusing column on th...

Page 21: ...0 to switch off the device Assembling the optics carrier 1 Place the optics carrier on the focusing column so that the screw fit into the thread provided and the lug fits into the groove 2 Press the o...

Page 22: ...ons 1 Unscrew the positioning screw and remove the protective cover Assembling the tube 2 Push the tube for example the inclined binocular tube into the dovetail ring and rotate it slightly in both di...

Page 23: ...lable 10 16 25 and 40 wide field eyepieces for persons wearing glasses Preparation 1 If you want to use an optional graticule insert it now page 65 2 Remove the plastic tube guard Inserting the eyepie...

Page 24: ...sem bly and disassembly so that it does not fall onto the stage plate This applies particu larly to the 2 planapochromatic objective which is very heavy Remove all specimens from the stage plate first...

Page 25: ...0 and attach the objective nosepiece to the drive hous ing from the front Screw the objective nosepiece firmly into place 3 Unscrew the three Phillips screws on the objective mount of the optics carri...

Page 26: ...aration O O Open the iris diaphragm O O Set the dioptric correction of the eyepieces to 0 Adjustment 1 Rotate the 2 objective into the beam path and set it to the lowest magnification 2 Focus on the s...

Page 27: ...Check the switch position O O If the switch position corresponds to the optics carrier used skip the following steps Otherwise proceed as follows 2 Unscrew the two screws that hold the switch in posi...

Page 28: ...ponding optics carrier it can be installed 1 Move the slide of the AX carrier into mid position 2 Install the AX carrier on the focusing column 3 Screw the objective to the AX carrier 4 Unscrew the th...

Page 29: ...ransport anchors Before you can use the transmitted light base for the first time it is abso lutely necessary to remove the two transport anchors as follows Transmitted light Base Leica TL BFDF Before...

Page 30: ...0 562 The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base withou...

Page 31: ...t or the right side of the XY stage If the controls are to be mounted on the left hand side the gear rod on the bottom side of the XY stage must be unscrewed and reattached in reverse 1 Take the glass...

Page 32: ...the glass plate from the XY stage and turn it around 2 Attach the axis with the control buttons to the desired side The fastener snaps into the XY stage magnetically 4 Attach the axis with the two All...

Page 33: ...stage all the way back towards the column 5 Reinsert the glass plate back into the stan dard stage Focusing drive and column 1 Unscrew the extension plate from the base using the Allen key provided 2...

Page 34: ...ace to the optics carrier is on the column The terminals The connection to the PC and to other instru ments is made using the terminals on the rear side of the column 1 3 CTL2 terminals for auxiliary...

Page 35: ...amera can be stowed in the cable duct Feeding the cables 1 Unscrew the three screws on the cable duct 2 Remove the cover of the cable duct 3 Place the cables in the cable duct and screw the cover on t...

Page 36: ...sembly 36 Cables Diagram SmartTouch PC USB Power USB LED5000 RL LED5000 MCI Please use the Leica Application Suite X to configure the functions of the system Alternative installation 115 230 V LED5000...

Page 37: ...Constraints TheLeicaLED5000MCIcannotbeusedtogether with the objective nosepiece Assembly 1 Hold the LED5000 MCI with one hand and tightly screw the retraining stirrups on both top holes on the drive...

Page 38: ...emain the same For such purposes the Leica LED5000 MCI is directly fastened to the column Installation on the column 1 Pull the retaining stirrup out of the Leica LED5000 MCI 2 Screw the retaining sti...

Page 39: ...planapochromat 1 and planapochromat 0 63 objectives With all other objectives the working distance is too low for adequate illumination The ring illuminator cannot be used together with the objective...

Page 40: ...ys for example if simultane ously using the Leica LED5000 MCI system illu minator In this case the ring illuminator cannot be connected directly to the Leica LED5000 MCI If you work with the manual fo...

Page 41: ...larization set into place firmly using the metal plate provided O O The optional polarization set polarizer and analyzer is attached and screwed in below the ring illuminator O O The optional diffuser...

Page 42: ...ont keypad O O Use the key to switch the illuminator on or off O O Use the or keys to adjust the bright ness in 10 increments O O Tap the or key briefly to adjust the intensity in small increments Hol...

Page 43: ...ress one of these keys again If you change the segment using the key the automatic changeover is kept Pressing or briefly stops the changeover O O Press and hold the key for approx 2 seconds to switch...

Page 44: ...he Leica LED5000 NVI in factory configu ration is already assembled with the exten sion plate for assembly on manual and motorized focusing columns at the factory 2 Place the Leica LED5000 NVI on the...

Page 45: ...e optics carrier on the adapter of the Leica LED5000 NVI and tighten it with an Allen key M4 6 Install the remaining microscope compo nents tube ergonomic accessories camera etc according to the corre...

Page 46: ...10 411 597 Achromatic objective f 100 mm 10 441 787 Achromatic objective f 150 mm 10 431 692 Achromatic objective f 175 mm 10 382 162 Achromatic objective f 200 mm 10 382 172 Achromatic objective f 4...

Page 47: ...e equipped with an optional polarization set Using the polariza tion set reflections can be reduced on metallic surfaces for example 1 The polarizer is inserted into the filter insert on the bottom of...

Page 48: ...1 2 can be used Make sure that the filters being used are suffi ciently temperature resistant so they are not damaged by the high energy illumination Additional empty filter inserts 10 725 035 can be...

Page 49: ...Leica M Series User Manual Quick Start Guide 49 Quick Start Guide...

Page 50: ...get right to work Therefore your next step should be to study the Quick Start Guide which outlines the most important steps at a glance This manual will then familiarize you with the finer details of...

Page 51: ...stops on off 3 Coarse fine focusing 4 Fastening screw for the binocular tube or accessories 5 Interchangeable objective 6 Adjustable eyepiece tubes 7 Eyepieces for spectacle wearers with dioptric cor...

Page 52: ...this Not to worry after a little while it will become automatic Reference value The distance between eye and eyepiece measures approx 22mm for 10 23B wide field eyepieces for persons wearing glasses A...

Page 53: ...eyepiece Using the Eyepieces If you do not wear glasses 1 Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise 2 If an eyepiece is equipped with the inte grated dioptric correctio...

Page 54: ...of the objective O O The focusing drive can be operated either left or right handed Focusing O O The inner coarse adjustment is used for covering great distances O O The outer fine adjustment is used...

Page 55: ...ocus movement too loose or too tight Does the outfit tend to slide downwards The resistance can be adjusted individually depend ing on the equipment weight and personal preferences as follows 1 Grip t...

Page 56: ...me indicates the zoom range covered O O Leica M125 C 12 5 1 O O Leica M165 C 16 5 1 O O Leica M205 C 20 5 1 Leica M205 A 20 5 1 The rotary knob for the zoom can be used either left or right handed Zoo...

Page 57: ...results etc can be reproduced more accurately Enabling and disabling ratchet steps 1 Push the top button downwards to disable the ratchet steps 2 Push the bottom button upwards to enable the ratchet s...

Page 58: ...t is located higher or lower Requirements for parfocal work O O If you are using an eyepiece with dioptric correction the procedure differs from this description For more information refer to page 64...

Page 59: ...the depth of field The depth of field is the area of a specimen that is brought into sharp focus Closing the iris diaphragm O O Close the iris diaphragm by turning the knob to the left The subject ap...

Page 60: ...Leica M Series User Manual Eyepieces 60 Eyepieces...

Page 61: ...ro scope but also has a critical effect on the maximum magnification The magnification factor is between 10 and 40 The following eyepieces are available for the M series Magnification Dioptric correct...

Page 62: ...epieces is a potential transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using individual eyepieces or detachable eyecups Eyecups can be ordered se...

Page 63: ...earing your glasses look through the eyepieces and focus on the specimen 3 Rotate both eyepieces to the maximum value of 5 4 Hold one eye closed and rotate the other eyepiece in direction until the sp...

Page 64: ...orrect setting of the diopters and the parfocality The following adjustments only have to be carried out once by each user Preparations O O Move the lever of the video phototube to the observation pos...

Page 65: ...ten ing it using the clamping screw Use with the AX carrier If possible measure with the micro scope carrier AX in vertical position The measurements are more accurate without the convergence angle in...

Page 66: ...Leica M Series User Manual Photography Video 66 Photography Video...

Page 67: ...stems offers a variety of adapters that can be used together with the 50 and 100 trinocular tubes Leica cameras However if you require absolute control over the camera and need the capability for meas...

Page 68: ...of the field of vision Cameras from third party suppliers In addition to Leica cameras with the standardized C mount interfaces you can connect third party cameras to the microscope using a T2 bayone...

Page 69: ...tube 50 enables you to simultaneously view and photograph a specimen The available light is divided as follows O O 50 is available for the two eyepieces O O 50 of the light is diverted to the video ph...

Page 70: ...sten the trinocular tube 100 to the optics carrier instead of the binocular observation tube refer to page 22 Switchover O O Turn the controller on the right side of the tube into the horizontal posit...

Page 71: ...es to best fit their posture The photo slider switches the right beam path from 100 of light to the eyepieces to 100 light to the camera port In photo position the left beam path is closed to prevent...

Page 72: ...Leica M Series User Manual Microscope Carrier 72 Microscope Carrier...

Page 73: ...l view and tighten the clamping screw The Microscope Carrier AX Users who intend to do a great deal of photog raphy take measurements or work with polar ization should select the microscope carrier AX...

Page 74: ...ill go and tighten the clamping screw The objective is now located directly beneath either the left or the right beam path Coaxial light and the Leica M205 C Leica M205 A When using coaxial incident l...

Page 75: ...es objectives the parfo cality is maintained even when objectives are changed meaning that the specimen remains in sharp focus during the change Older Leica objectives can continue to be used but with...

Page 76: ...Leica M Series User Manual Objectives and Optical Accessories 76 Objectives and Optical Accessories...

Page 77: ...atic objectives the finest structures are visible with high contrast The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity Achromatic ob...

Page 78: ...Leica M Series User Manual Bases 78 Bases...

Page 79: ...ases 79 Transmitted light Base Leica TL BFDF Controls Extensionplateofthetransmitted lightbaseTLBFDF Button to toggle between bright field dark field Connector for cold light sources light conductor a...

Page 80: ...cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background O O Turn the control as far as it will go tow...

Page 81: ...User Manual Bases 81 Leica IsoPro Non motorized Controls Operating the XY stage IsoPro 1 To move the stage in X direction rotate the outer knob 2 To move the stage in Y direction rotate the inner con...

Page 82: ...Leica M Series User Manual System Illumination 82 System Illumination...

Page 83: ...e bottom end of the guide rods In this position you always have the same contrast with identical illumination This guar antees the reproducibility of an experiment Contact with the base If the optics...

Page 84: ...keys to toggle between the illumination scenarios shown below Maximum brightness For temperature reasons it is not possible to switch on all nine LEDs simultaneously There fore to provide bright over...

Page 85: ...utomatically toggle between them For additional information refer to the LAS X online help Using the keyboard O O Use the key to switch the illumination on or off O O Use the and keys to adjust the br...

Page 86: ...Leica M Series User Manual Accessories 86 Accessories...

Page 87: ...o five foot switches can be configured with a microscope system They can be programmed in LAS X to control focus illumination and indi vidual memory positions Leica Hand Wheel and Foot Switch The hand...

Page 88: ...ith freely programmable rotary knobs and function buttons Controls The functions of the individual knobs and screen elements can be configured both directly on the Leica SmartTouch and using the Leica...

Page 89: ...Leica M Series User Manual Dimensional Drawings 89 Dimensional Drawings...

Page 90: ...ual Dimensional Drawings 90 Leica M125 C Leica M165 C 120 120 24 455 292 217 120 120 277 24 400 455 292 217 120 120 277 24 400 455 Leica M125 C Leica M165 C with incident light base and new focusing c...

Page 91: ...eica M165 C Continued 496 5 91 431 118 187 154 300 50 mm 496 5 91 431 522 118 187 154 300 370 Y 50 mm Y 75 mm 496 5 91 431 522 118 187 154 300 370 Y 50 mm Y 75 mm Leica M125 C Leica M165 C with transm...

Page 92: ...User Manual Dimensional Drawings 92 Leica LED5000 MCI 132 275 3 157 83 5 112 45 112 90 45 36 22 132 275 3 157 83 5 112 45 112 90 45 36 22 132 275 3 157 83 5 112 45 112 90 45 36 22 Leica LED5000 MCI di...

Page 93: ...Leica M Series User Manual Dimensional Drawings 93 Leica LED5000 RL 132 132 160 37 160 37 58 mm Leica LED5000 RL dimensions in mm...

Page 94: ...Leica M Series User Manual Dimensional Drawings 94 Leica LED5000 NVI Leica LED5000 NVI for high performance stereo microscopes dimensions in mm...

Page 95: ...Leica M Series User Manual Dimensional Drawings 95 Leica MATS TPX 190 140 220 4 11 134 98 170 10 447 164 10 447 275...

Page 96: ...Leica M Series User Manual Specifications 96 Specifications...

Page 97: ...planapochromatic 28 8 mm 2 3 mm 7 3 120 max 453 lp mm 61 5 mm planapochromatic 31 5 mm 1 92 mm 7 8 160 max 525 lp mm 61 5 mm planapochromatic 29 5 mm 1 44 mm Maximum values based on optics combinatio...

Page 98: ...from 1 000 V to 100 V Coded motorized function Zoom iris diaphragm objective nosepiece coded only Zoom iris diaphragm objective nosepiece coded only Zoom iris diaphragm objective nosepiece encoded an...

Page 99: ...change 5 12 200 parfocal Fast magnification change 4 67 240 parfocal Fast magnification change 4 99 320 parfocal Binocular tubes ergonomics Apochr binocular and trinocular tubes with synchronized inte...

Page 100: ...e Leica IsoPro XY stage manual Illuminators LED5000 RL LED5000 SLI LED5000 MCI LED5000 HDI LED5000 NVI LED5000 CXI cold light sources Misc accessories Photography video Digital image capture systems f...

Page 101: ...lumination types Bright field Yes Dark field Yes Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control Yes Integrated filter holder No Co...

Page 102: ...idity operating storage 10 90 RH non condensing Operating altitude maximum 0 2 000 m Storage altitude nA Pollution degree of intended environment 2 office lab environment Accessories Various Leica con...

Page 103: ...Leica M Series User Manual Appendix 103 Appendix...

Page 104: ...planachromatic and planapochromatic objectives of the MZ125 MZ16 are used on the MS5 MZ6 MZ75 or MZ95 NFOV Field number of the eyepiece Field numbers are printed on the eyepieces 10 21 16 14 25 9 5 4...

Page 105: ...lting from careless or improper handling Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Switzerla...

Page 106: ...ents Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuit able cleaning agents and techniques can damage polymers Permitted...

Page 107: ...to modifications LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH CONNECT WITH US www leica microsystems com Leica Microsystems Schweiz AG Max Schmidheiny Strasse 201...

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