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Instrument Information 

Contrast Fields: Bright Field, Dark Field, Differential Interference Contrast, Polarizing-Contrast 
Magnification: 5X, 10X, 20X, 50X, 100X 
Focus Increments: .05 µm .1 µm, .7 µm, 1.5 µm, 5.0µ 
Stages: 150 mm x 200 mm 

 
Preparing the Machine 

Physical Setup 

1)

 

Turn on the optical microscope then turn on the computer  

a.

 

The power switch is on the CTR advanced box

 

2)

 

Wait a few moments for the DMi8A to self-align and go 
through its boot-up sequence 

 
Computer Setup 

1)

 

Double click on the LAS V4.7 computer icon 

a.

 

The icon is the Leica Application Suite as to which the 
DMi8A connects to.  

2)

 

If you only see black or white on the live feed, adjust the light 
intensity to see your sample.  

a.

 

Small knob on the left of the optical microscope 
 

 

Figure: The Main Menu for the Optical Microscope. Areas of interest have been 

marked with an arrow. 

Figure: The Icon for the Leica 

Application Suite program. This icon 

links to the software for Leica’s 

Summary of Contents for DMi8A

Page 1: ...1 Leica DMi8A Quick Guide...

Page 2: ...defined Operating the Microscope 4 Navigation 4 Adjusting the Filtering Parameters 4 Getting Results 6 Taking Pictures 7 Editing Pictures Error Bookmark not defined Finishing Up 8 Turning off the Sys...

Page 3: ...ait a few moments for the DMi8A to self align and go through its boot up sequence Computer Setup 1 Double click on the LAS V4 7 computer icon a The icon is the Leica Application Suite as to which the...

Page 4: ...lds The different scanning fields reflect numerous forms of illumination techniques used within advanced microscopes Incorrect illumination of the sample can lead for sophisticated microscopes to take...

Page 5: ...to samples DIC Highlights the otherwise invisible features of a sample Very useful for observing unstained samples that need to be stained with the other scanning fields Enhanced light and shadow may...

Page 6: ...ter images with more noise Dimmer images with less noise Saturation Intensity of the color to white Images will have more purer colors Images will have more washed up colors Gamma Factor between linea...

Page 7: ...r image for any second thoughts on the way that the image s camera controls were captured Taking and Saving Pictures 1 On the right side of the window adjust your image size to your desired dimensions...

Page 8: ...long the X Y axis or a degree based rotation Analyzing Pictures 1 At the top of the screen select the Interactive window within the Analysis tab 2 Here you will see an assortment of tools as listed Se...

Page 9: ...far away you want the label from the mark 4 Within the Automatic and Grain Expert windows you can follow the computer prompted steps to automatically analyze your picture and generate a report based o...

Page 10: ...ensure a neat environment Tips and Tricks Have curves in your path that you want to analyze Hold the LMB on the mouse and move for a continuous path to see your total distance Want to see two image p...

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