38
6. Startup
• Open the field diaphragm just enough for it to
disappear from the field of view (41d).
Caution:
Do
not
adjust the aperture diaphragm. The aper-
ture diaphragm is already set optimally for each
objective.
6.6. Checking Phase Contrast Rings
A
B
C
D
Fig. 41
Köhler Illumination
a
Field diaphragm not focused, not centered
b
Field diaphragm focused, but not centered
c
Field diaphragm focused and centered
Diameter is too small, however
d
Field diameter (light) = Field diameter (view)
(Köhler Illumination)
Fig. 40
Condenser centering
1
Centering bolts
1
1