53
Imaging the light sources to check adjustment
Projection without objective (method 1)
Put a piece of paper or similar on the specimen
stage and roughly focus the surface with a dry
objective of low to medium magnification. Set
the field and aperture diaphragms (39.6 and 39.8)
roughly at the centre position. With a felt or
ballpoint pen, make a dot or cross at any
position on the paper and slide it into the small
illuminated field. Maybe fix the paper in position.
Turn a vacant nosepiece position into the light
path or remove the objective: The light source
(38, 41) will then be imaged on the paper via the
beamsplitter/filter cube. Cont.
→
p. 54.
Projection on the microscope base (method 2)
Pull the light stop* (30.5) out of the stage (39.4).
Center the condenser at least roughly
→
p. 37.
Remove the specimen. Turn a 4x, 5x or 10x
objective into the light path. Switch the
condenser disc* to the BF position (7.3) or pull
out the light ring slide (7.7). Open the aperture
diaphragm in the condenser (28.14). Put a piece
of paper on the microscope base. Adjust the
height of the stage (28.7) or condenser (28.4)
until the bright circle (= image of the objective
pupil) is quite sharply defined. Set the field and
aperture diaphragms in the illuminator (39.6 and
39.8) roughly at the centre position. Mark the
middle of the bright area.
Fig. 39
Incident light fluorescence illuminator LRF 4/22
1a, b
Fixing screws,
2
4-position filter wheel,
3
Cover, with
indentation grip x in housing,
4
Light trap* (light stop for
transmitted light), insertable in mechanical stages, concealed
in the illustration,
→
(30.5),
5
Holes for centering keys (field
diaphragm),
6
Field diaphragm adjustment,
7
Holes for
centering keys (aperture diaphragm),
8
Aperture diaphragm
adjustment,
9
Light stop (dark flap),
10
BG 38 filter,
11a, b
Fixing screws for flip-up lid of lamphousing 105 z,
12
Cut-out
plug,
→
Fig. 22.11
1a
2
3
1b
4
5 6 7 8 9 10
11a 12 11b
Summary of Contents for DM L
Page 1: ...Leica DM L Instructions...
