Leica BIOSYSTEMS SM2000R Instructions For Use Manual Download Page 3

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Leica SM2010 R

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19

D –69226 Nussloch
Germany
Phone: +49 6224 143–0

Fax: +49 6224 143–268

Internet: http://www.LeicaBiosystems.com

Assembly contracted to Leica Microsystems Ltd. Shanghai

The information, numerical data, notes and value

judgments contained in this manual represent the

current state of scientific knowledge and state-of-

the-art technology as we understand it following

thorough investigation in this field.

We are under no obligation to update the present

manual periodically and on an ongoing basis ac-

cording to the latest technical developments, nor

to provide our customers with additional copies,

updates etc. of this manual.

To the extent permitted in accordance with the

national legal system as applicable in each indi-

vidual case, we shall not be held liable for errone-

ous statements, drawings, technical illustrations

etc. contained in this manual. In particular, no

liability whatsoever is accepted for any financial

loss or consequential damage caused by or

related to compliance with statements or other

information in this manual.

Statements, drawings, illustrations and other

information as regards contents or technical

details of the present Instructions for Use are not

to be considered as warranted characteristics of

our products.

These are determined only by the contract

provisions agreed between ourselves and our

customers.

Leica reserves the right to change technical spec-

ifications as well as manufacturing processes

without prior notice. Only in this way is it pos-

sible to continuously improve the technology and

manufacturing techniques used in our products.

This document is protected under copyright laws.

All copyrights to this documentation are held by

Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of

any parts thereof) by means of print, photocopy,

microfiche, web cam or other methods – includ-

ing any electronic systems and media – requires

express prior permission in writing by Leica Bio-

systems Nussloch GmbH.

For the instrument serial number and year of

manufacture, please refer to the name plate at

the rear of the instrument.

NOTE

Summary of Contents for SM2000R

Page 1: ...se Leica SM2010 R Sliding Microtome Instructions for Use Leica SM2010 R V 1 4 RevC 09 2013 English Order No 14 0508 80101 RevC Always keep this manual with the instrument Read carefully before working with the instrument ...

Page 2: ......

Page 3: ... consequential damage caused by or related to compliance with statements or other information in this manual Statements drawings illustrations and other information as regards contents or technical details of the present Instructions for Use are not to be considered as warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and our cu...

Page 4: ...their functions 21 5 1 1 Section thickness setting 21 5 1 2 Coarse driving wheel 21 5 1 3 Manual feed 22 5 1 4 Automatic feed 22 5 1 5 Directional fixture for specimen clamps 23 5 2 Clamping the specimen in the universal cassette clamp UCC 24 5 3 Clamping a disposable blade 25 5 4 Replacing the pressure plate 26 5 5 Inserting the knife 27 5 6 Sectioning 28 5 7 Changing the specimen or interrupting...

Page 5: ...side of the instrument The figure below is provided as an example only and shows a valid nameplate for this instrument Qualification of personnel The Leica SM2010 R may be operated by trained laboratory personnel only Fig 1 Warnings appear in a gray box and are marked by a warning triangle Notes i e important user information appears in a gray box and is marked by an infor mation symbol Numbers in...

Page 6: ...rily responsible for accident free operation is above all the owner of the instrument and in addition the designated personnel who operates services or cleans the instrument To ensure trouble free operation of the instrument make sure to comply with the following instructions and warnings Be sure to comply with the safety instructions and warnings provided in this chapter Be sure to read these ins...

Page 7: ...he knob for setting the section thickness Be very careful when handling microtome knives or blades The cutting edge is extremely sharp and can cause serious injuries Always wear work safety shoes and safety gloves Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife Always put the knives back into the knife case when not in use Always clamp the s...

Page 8: ... the locking knob 23 that engages inthenotchpointsonthebar 24 thus holding the knife sledge securely The bar has 11 notch points with a dis tance of 10 mm between each 14 14 11 Knife guard on the knife holder Knife guard on the blade holder Knife sledge locked Knife sledge is movable Prior to any manipulation of the knife or specimen or each change of specimen and during breaks always cover the cu...

Page 9: ...ge immobilizer Setscrew for orientation in cutting direction Adjustable instrument feet HN40 tensioning clamp Lever for clamping the blade Scaled adjusting knob for setting the section thickness Lever for manual feed Clamping lever for lateral displacement Clamping lever for specimen orientation Knife sledge lock Clearance angle scale Knife holder SN Setscrew for orientation perpendicular to cutti...

Page 10: ...rements from 5 0 10 0 µm in 1 0 µm increments from 10 0 20 0 µm in 2 0 µm increments from 20 0 60 0 µm in 5 0 µm increments Automatic specimen feed from 0 to 30 µm Total specimen stroke approx 50 mm Clearance angle adjustment 3 to 10 Maximum specimen size 50 x 60 x 40 mm Specimen orientation in cutting direction 8 perpendicular to cutting direction 8 Declination 0 45 in cutting direction Dimension...

Page 11: ...Ergo handle for easily moving the sledge The sectioning window can be adjusted to the specimen size The cutting thickness can be adjusted within the range of 0 5 µm to 60 µm the automatic feed functions in the range of 0 5 µm to 30 µm Manual feed by pulling or pushing the feed lever Depending on the version the instrument is either fitted with a blade holder SE for disposable blades or a knife hol...

Page 12: ...ry includes 1 Leica SM2010 R basic instrument 14 0508 42258 1 section waste tray 14 0508 42328 1 tool set consisting of 14 0508 42983 1 Allen key size 6 14 0194 43634 1 Allen key with handle size 4 14 0194 04782 1 Allen key T 25 14 0194 45250 1 open end wrench size 10 14 0330 04158 1 spare magnet 14 0508 44762 1 dust protection cover 14 0212 18961 1 pair of safety gloves size S 14 0340 40859 1 Ins...

Page 13: ...p First check the shipment for external damages upon arrival If it is evident that the shipment was damaged during transport please make a claim to the carrier immediately Open the packaging Remove all foam material Take out all accessories and the instruction manual Do not transport the instrument by hold ing it by moving parts on the knife sledge coarse driving wheel or the knob for setting the ...

Page 14: ...strument feet 25 on the right side of the instrument are height adjustable For alignment loosen the locknuts 26 using a size 10 open end wrench Adjust the instrument feet 25 until the micro tome is in a stable position at the installation location according to requirements Retighten the locknuts Setting the Ergo handle The Ergo handle 12 which is used to move the knife sledge can be set individual...

Page 15: ...etheintermediateplate on the mounting table Make sure that the two index markings 65 are at the top and point towards the back Insert the five countersunk screws 61 1 61 5 into the bores and tighten them crosswise in the sequence shown in Fig 10 using an Allen key T25 63 23 23 60 62 65 64 1 63 61 3 61 5 61 1 61 2 61 4 64 2 11 4 Instrument Setup Fig 9 Important The mounting table must be absolutely...

Page 16: ...continued 4 Instrument Setup Continue turning the clamping lever 66 in a clockwise direc tion until the knife holder is firmly screwed on Fig 12 67 60 The clamping lever 66 has a plastic handle that can be turned into what ever position is best To do so pull the handle up wards and turn it into the desired position Fig 12 It will then lock automati cally when released The knife holder SN 67 has tw...

Page 17: ...nurled head screw 69 Set the desired angle as described above To fix the setting in place retighten the knurled screw 69 Enlarged detail Scale for setting the clearance angle 72 69 70 73 4 Instrument Setup Inclination declination of the knife holder SN Loosen the clamping lever 66 Set the required declination inclination of the knife holder from the cutting direction on the scale 55 1 at the back ...

Page 18: ...d opening 56 for the T piece Fig 16 pointing forward Insert the six countersunk screws 61 1 61 6 into the bores and tighten them crosswise in the sequence shown in Fig 16 using an Allen key T25 63 23 63 61 3 61 5 61 1 61 2 61 4 61 6 56 The blade holder SE is designed for conventional disposable blades from all common manufactur ers It is available in two models one for low profile blades and one f...

Page 19: ... Allen key T25 63 To clamp the blade holder on the grooved plate tighten the screw 52 on the back of the blade holder base 17 using a size 6 Allen key Fig 19 Fig 17 Fig 18 18 54 Mount the blade holder 9 on the grooved plate 50 such that the T piece 53 on the underside is inserted in the groove 54 56 9 52 63 The stopper 18 serves for clos ing the insertion opening 56 for the T piece in the grooved ...

Page 20: ...hen adjusting the declination For fixation tighten the screw 52 firmly in the required position Fig 19 52 55 2 55 1 Assembling the blade holder SE continued 19 27 27 1 Lateral movement The lateral displacement feature enables the en tire cutting length of the blade to be used without having to readjust the blade holder To move the blade rail turn the clamping lever of the lateral displacement mech...

Page 21: ... the segment arc until the index mark coincides with that of the desired setting Example Enlarged detail showing a clearance angle setting of 4 13 43 1 43 2 Enlarged detail Scale for setting the clearance angle 15 17 52 52 20 Assembling the blade holder SE continued 4 Instrument Setup Caution Always remove the blade before adjust ing the clearance angle The knife guard does not provide any protect...

Page 22: ...object head 40 to the lower end po sition by turning the coarse driving wheel 36 To loosen the clamping system turn the screw 37 of the quick clamping system 29 coun terclockwise using a size 4 Allen key 39 Push the guide 42 of the universal cassette clamp 41 from the left into the quick clamping system 29 as far as it will go To clamp the cassette clamp turn the screw 37 clockwise as far as it wi...

Page 23: ...ss setting 5 1 2 Coarse driving wheel The coarse feed serves for fast vertical upward movement of the object towards the knife and downward movement away from the knife The coarse driving wheel 36 has a direction selection lever 37 with which you can select the direction of rotation upwards feed movement of the specimen towards the knife Position of the direction selection lever for feed motion 38...

Page 24: ...mediately in front of the specimen 33 35 5 6 22 22 23 Fig 24 Fig 25 If the adjusting button 22 is locked in the frontmost left position Fig 25 there is no feed motion The automatic feed is only effective up to a section thickness of 30 µm All val ues set beyond that are not defined To adjust the automatic feed move the blade knife 6 until it is just in front of the specimen 5 Fig 25 and lock it in...

Page 25: ...r orientation there is a noticeable notch point after each complete turn of the setscrew To lock the current orientation turn the ec centric lever 34 backwards Display of the zero position For better display of the zero position each set screw 30 31 has a red mark 32 When both marks are visible and both setscrews areinzeropositionatthesametime notchpoint the specimen orientation is in zero positio...

Page 26: ...ever 44 upwards to open the clamp Insert the cassette 45 into the cassette clamp To clamp the cassette release the lever 44 41 44 14 14 45 45 Caution Always clamp the specimen block BE FORE clamping the knife Lock the knife sledge and cover the knife edge blade edge with the knife guard prior to any manipulation of knife blade or specimen prior to changing the specimen block and during all work br...

Page 27: ...h the pressure plate Push the clamping lever 46 downwards to clamp the blade Make sure that the blade is clamped parallel to the front edge of the pressure plate Removal aid Fig 29 A removal aid is provided for removing used blades 6 Loosen the clamping lever 46 of the pressure plate 48 Pushtheknifeguard 14 towardstheleftwhile holding down the black push button 51 This pushesout thebladefarenoughs...

Page 28: ...ht and push the lever 46 upwards to release the clamp of the pressure plate 48 Now carefully pull the insertion aid 57 out to the left Fig 30 The pressure plate 48 can now be taken off Fig 31 To mount another pressure plate please proceed in the reverse sequence Only use the pressure plate together with the matching insertion aid 21 Fig 32 Fig 30 Fig 31 48 46 14 57 Pressure plate kit 48 57 Inserti...

Page 29: ... to be inserted Take the knife out of the knife case and insert it carefully Tighten the two clamping screws 72 in alternation until both are secure and cover the knife with the knife guard 5 5 Inserting the knife 72 67 66 69 72 11 23 67 5 Operation Be very careful when han dling microtome knives or blades The cutting edge is extremely sharp and can cause serious injuries The blade holder has to b...

Page 30: ...pecimen feed by the selected value Move the knife sledge forth and back until the specimen surface is trimmed as required 5 Operation 5 6 Sectioning Remove the sections Always use different areas of the cutting edge for trimming and sectioning Select the required section thickness with the section thickness adjusting knob 33 To produce the section pull the knife sledge over the specimen at a const...

Page 31: ... remove the knife from the knife holder and put it back in the knife case Remove the specimen from the specimen clamp Push all section debris into the section waste tray and empty the tray Clean the instrument see Chapter 8 1 Lock the knife sledge and cover the knife edge with the knife guard prior to any manipulation of knife or specimen head as well as prior to changing the specimen block and du...

Page 32: ...ste tray and remove the section waste with a dry brush Remove specimen clamp and clean separately Always remove the knife or blade before detaching the knife blade holder from the instrument Always put the knives back into the knife case when not in use Never place a knife anywhere with the cutting edge facing up wards and never try to catch a falling knife When using cleaning agents observe the m...

Page 33: ...n please contact your local Leica technical service center 6 Cleaning and Maintenance 6 2 Maintenance instructions Clean the instrument and outer surfaces If necessary the varnished outside surfaces can be cleaned with a mild commercial household cleaner or soapy water and then wiped with a moist cloth To remove paraffin residue xylene substitutes e g Roth Histol Roth Karlsruhe Tissue Clear Medite...

Page 34: ...mbly 14 0508 43196 Blade holder SE BB assembly 14 0508 42775 Pressure plate kit SB assembly 14 0508 43693 Pressure plate kit BB assembly 14 0508 43694 Low profile blade rail EC 240 L 14 0368 33013 Low profile blade rail set assembly 14 0368 38111 Plastic knife guard for blade rails 14 0368 33772 Knife 16 cm profile C steel 14 0216 07100 Knife 16 cm profile D steel 14 0216 07132 Knife 22 cm profile...

Page 35: ... 42634 Universal cassette clamp UCC with adapter silver for mounting in the quick change system for specimen imaging Maximum specimen size 40x29 mm LxW Order No 14 0508 42635 HN40 tensioning clamp with adapter silver for mounting in the quick change system for specimen imaging Maximum specimen size 59x45 mm LxW Order No 14 0508 42637 7 Optional Accessories Fig 36 Fig 37 Fig 38 ...

Page 36: ...in the quick change system for specimen imaging Maximum specimen size 79x60 mm LxW Order No 14 0508 42632 Dry ice tub with adapter silver Order No 14 0508 42641 1 pair of cold protection gloves size 8 Order No 14 0340 45631 7 Optional accessories Dust cover Order No 14 0216 07100 Fig 39 Fig 40 Fig 41 ...

Page 37: ...aid integrated into the knife guard Space saving knife guard with integrated blade removal tool in signal color Standard delivery 1 blade holder base 14 0508 44719 1 grooved plate 14 0508 43643 1 stopper 14 0508 44664 6 countersunk screws Torx 14 3000 00227 Blade holder for SB Order No 14 0508 43196 Blade holder for BB Order No 14 0508 42775 Fig 42 Pressure plate kit SB assembly consisting of 1 pr...

Page 38: ...inte grated knife guard in signal color Standard delivery 1 intermediate plate 14 0508 44671 5 cheese head screws 14 0508 44670 1 knife holder attachment SN 14 0508 44857 Order No 14 0508 44670 Low profile blade rail EC 240 L for low profile disposable blades shipment in a plastic case with two pressure plates and all required tools and accessories see Fig 45 Order No 14 0368 33013 Low profile bla...

Page 39: ...nd frozen sections Note including knife case 14 0213 11140 Order No 14 0216 07100 Fig 48 Knife 16 cm Profile D steel Note including knife case 14 0213 11140 Order No 14 0216 07132 Knife 22 cm Profile C steel for paraffin and cryosections Note including knife case 14 0213 11141 Order No 14 0216 07116 Fig 49 Fig 50 Ball handle assembly Order No 14 0508 42565 Fig 51 ...

Page 40: ...file disposable blades Type 819 Length 80 mm height 8 mm 1 package of 50 pieces Order No 14 0358 38925 Leica high profile disposable blades Type 818 Length 80 mm height 14 mm 1 package of 50 pieces Order No 14 0358 38926 7 Optional accessories Fig 52 Fig 53 ...

Page 41: ... is too warm The clearance angle is too wide Use another area of the blade knife or use a new blade knife Cool the specimen before section ing Methodically reduce the clearance angle setting until you have found the optimum angle 3 The sections have scratches and chatter marks The clearance angle is too wide Unsuitable knife profile Insufficient clamping to the object holder system and or the blad...

Page 42: ...ty terms of your Leica sales organization or the organiza tion from which you have purchased the contractual product shall apply exclusively Service information Ifyouareinneedoftechnicalcustomerserviceorspareparts pleasecontact your Leica representative or the Leica dealer where you purchased the unit Please provide the following information Model name and serial number of the instrument Location ...

Page 43: ...41 Leica SM2010 R 10 Notes ...

Page 44: ...ut proper decontamination documentation If you have any further questions please call your local Leica organization Leica Internal Use If applicable note corresponding Job and RAN RGA Number Job Sheet No _______________ BU Return Authorization Number ______________ SU Return Goods Authorization ______________ 2013 Order No 14 0700 83101 DearCustomer AnyproductthatistobereturnedtoLeicaBiosystemsors...

Page 45: ...partment Address Phone Fax Important to avoid refusal of shipment Place one copy in the unit prior to packaging or hand it over to the service engineer Customer assumes all responsibility for the immediate return shipment of articles sent to Leica without proper decontamination documentation If you have any further questions please call your local Leica organization Leica Internal Use If applicabl...

Page 46: ...www LeicaBiosystems com Leica Biosystems Nussloch GmbH Heidelberger Straße 17 19 D 69226 Nussloch Phone 49 0 6224 143 0 Fax 49 0 6224 143 268 Web www LeicaBiosystems com ...

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