14
3
4
Adjusting the Focus
Using the Eye Shades
Objective
Magnification
4X
10X
40X
100X
WD (mm)
22
10.5
0.56
0.13
WD
3
2
1
Fig. 13
Sigma
5
Switching the Objectives
Fig. 15
1
Focusing Procedure (Fig. 13)
1. Rotate the coarse adjustment knob (1) clockwise so that the objective (3) is as
close as possible to the specimen (We recommend starting with 10X).
2. While observing the specimen through the eyepiece, slowly rotate the coarse
adjustment knob (1) counterclockwise to lower the stage.
3. When coarse focusing of the specimen is obtained (an image is detected),
rotate the fine adjustment knob (2) for fine focusing.
Working Distance (WD)
The WD refers to the distance between each objective and the specimen, when
precise focus of the specimen is obtained.
Using the Eye Shades
When Wearing Eyeglasses
Use with the eye shades in the normal, folded-down position. This will
prevent the eyeglasses from being scratched.
When Not Wearing Eyeglasses
Extend the folded eye shades outwards (direction of the arrow) to
prevent extraneous light from entering into your line of vision.
Hold and rotate the revolving nosepiece turret (1) so that the objective
to be used is in line above the specimen. Always use the ribbed grip to
rotate the objective nosepiece.
Summary of Contents for Sigma
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