background image

Rotate the revolving nosepiece (1) so that the objective to be used is in line 
above the specimen. Always use the knurled surface to rotate the objective 
nosepiece.

6

Switching the Objectives

Fig. 35

The designated immersion oil should be in contact with the cover lens of 
the 100X immersion objective. If not, the specimen will appear distorted 
and dull.  It is recommended that LABOMED immersion oil is always used.

Immersion Process:

1. Bring the specimen in focus using first the 10x, then 40x objective.

2. Disengage the 40x cycling towards 100x, and place a drop of immersion 
    oil on the center point of the specimen.

3. Rotate the revolving nosepiece to engage the immersion objective and   
    rotate the fine adjustment knob to bring the specimen into focus

(Since air bubbles in the oil will affect the image quality, make sure 
that the oil Is free of bubbles. To remove bubbles, rotate the revolving 
nosepiece slightly to agitate the oil).

4. The condenser of this microscope manifests the full performance when 
    oil is placed between the slide glass and the front lens of condenser. If oil 
    is not placed there, the observed image may appear dark.
5. After use, remove oil from the objective front lens by wiping with lens 
    tissue slightly moistened with an ether (70%) alcohol (30%) mixture.

Caution 
If  immersion  oil  makes  contact  with  your  eyes,  rinse  eyes  out 
thoroughly  with  fresh  water.  If  immersion  oil  makes  contact  with 
skin, wash affected areas with soap and water.
If  prolonged  discomfort  is  experienced,  consult  your  physician 
immediately.

7

Using the 100X Immersion Objective

Lx POL

LABOMED

        

USA

RP POL PLAN ACHRO

40X / 0.65 / 0.17

9124040

LABOMED

        

USA

RP POL

 PLAN 

ACHRO

10X / 0.25 / 0.17

9124010

R

Fig. 36

1

24

Summary of Contents for Lx POL

Page 1: ...re proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended User Manual Lx POL Research Microscopy R R...

Page 2: ......

Page 3: ...YOUR MICROSCOPE 8 4 Lx POL TRINOCULAR 7 3 Lx POL BINOCULAR 6 7 OPTIONAL ACCESSORIES INSTALLATION 10 11 8 DETAILED INTRODUCTION OF ASSEMBLIES 12 14 a Flip Top Abbe Condenser b Round Stage c Centerable...

Page 4: ...t 11 SUMMARY OF POLARISED OBSERVATION PROCEDURE 20 12 SUMMARY OF POLARISED OBSERVATION PROCEDURE 21 24 a Placing the specimen on stage b Adjusting the focus c Adjusting the Interpupillary Distance d A...

Page 5: ...bjectives The round ball bearing Rotatable Stage has smooth 360 degree travel and has 1 degree graduations on the scale which provides accurate location of the specimen High power illumination is deli...

Page 6: ...Lx POL if the microscope is used in a manner not specified by this manual the safety of the user may not be warranted In addition the equipment may also suffer damage Always use the equipments as out...

Page 7: ...ng Methanol Place the Objectives and or eyepieces on a dust free surface e g aluminium foil All other optical components to be cleaned should be as accessible as possible 3 Blow all loose dust particl...

Page 8: ...oscope on a sturdy level table or bench and aovid any restriction of air vents in the base of the unit Do not place microscope on a flexible surface as this could result in blocking the air vents and...

Page 9: ...bulb reduce its life If contamination occurs wipe bulb surface with a cloth slightly moistened with alchohol Applicable Bulb 6V20W Halogen Bulb P N CX 013 Fig 1 Caution For Fuse replacement Set the m...

Page 10: ...der Vernier Scale Flip Top Abbe Condenser Polarizer Koehler mount Coarse and fine focus knob Polarizer locking screw Intensity regulator Bertrand lens focusing knob Bertrand lens on off Allen keys for...

Page 11: ...king screw Circular stage with vernier Port of wave plate Analyzer on off Parking for additional wave plates 2 Allen keys for objective centering Trinocular viewing tube 30 inclined Bertrand lens focu...

Page 12: ...8 UNPACKING YOUR MICROSCOPE 5 Microscope Arm Observation head Eyepieces Power Cord...

Page 13: ...Paired Eyepieces 6V 20W Halogen bulb R Binocular viewing tube 30 inclined Eyepieces Intermediate Analyzer Kit Bertrand Lens centering screw Centerable Revolving Nosepiece Polarizing Objectives Specime...

Page 14: ...ories OPTIONAL ACCESSORIES 7 Binocular head Flip Top Abbe Condenser Blue filter RP 2 5x RP 10x RP 20x RP 40x SL RP 100x SL Oil Trinocular head WF 10x WF 16x WF 20x Halogen Bulb iVu 5100 iVu 7000 R R R...

Page 15: ...ar observation head Fig 5 2 Mount iVu Camera Module System Fig 3 on video adapter 1 iVu Camera Module System 10X eyepieces are provided To replace 1 Pull out the 10x eyepieces out from the observation...

Page 16: ...scope s optical axis This makes the center of rotation coincide with the center of field of view Rotatibility allows the user to observe the specimen in the diagnol position the brightest position of...

Page 17: ...another Lx POL Nose Piece is fully equipped with centering mechanism for each objective to make sure that each object is centered to the stage and microscopic optical axis so that the specimen remains...

Page 18: ...rience Fig 13 14 1 Bertrand Lens Focusing Knob 2 Bertrand Lens Operating Lever 3 Analyzer Operating Lever 4 Gypsum wave Plate 5 Allen wrench for Objective centering 6 Allen screw for Bertrand Lens Cen...

Page 19: ...Locking Screw 1 See figure 15 3 Remove the Dust Cover Cap from Observation Head Dovetail and mount the Observation Head by engaging the dovetail provided at the bottom of the head into the dovetail c...

Page 20: ...b in the opposite direction decreases brightness The intensity bar next to the knob indicates the direction of intensity level Note Never use an adapter between the power cord and the power source it...

Page 21: ...diaphragm so that its closed iris leaves image is present within the field of view Use the condenser focusing knob to bring the image into sharp focus Operate the condenser centering screws simultane...

Page 22: ...ntil the prominent point of the specimen is furthest away from the center of the Eye Piece crossline it may even be outside the field of view Fig 24 b iii Adjust the image with the centering screws ov...

Page 23: ...n 1 Bring an objective of your choice between 20x to 100x in the light path Bring the Pol specimen into focus 2 Keep the abbe condenser in its lowest position 3 Swing inTop lens of FlipTopAbbe Condens...

Page 24: ...o Eye Piece cross hair scale Adjust the observation tube and Adjust the interpupillary distance Adjust the dioptric setting Engage Polarizer Analyzer filters in the light path and use Bertrand Lens an...

Page 25: ...g position The degree of position can be noted Vernier Scales These scales allow for easy identification of the specimen s position coordinates making it easy to return to a particular region of inter...

Page 26: ...tereo vision This greatly helps to reduce fatigue and discomfort during observation While looking through the eyepieces move both eyepieces laterally until the left and right fields of view coincide c...

Page 27: ...nto focus 3 While looking through the left eyepiece with your left eye rotate only diopter adjustment ring on the eyepiece until specimen is at its best possible focus The condenser is most often used...

Page 28: ...to focus Since air bubbles in the oil will affect the image quality make sure that the oil Is free of bubbles To remove bubbles rotate the revolving nosepiece slightly to agitate the oil 4 The condens...

Page 29: ...th an Use immersion oil as suggested immersion objective Bubbles are present in immersion oil Remove the bubbles by agitation The specified immersion oil is not used Use the immersion oil supplied by...

Page 30: ...Check and replace with live fuse 10 Bulb blows easily The specified bulb is not used Replace with the specified bulb 11 Field remain dark even Bulb is on In the Exintetion Position Remove Analyzer fr...

Page 31: ...ty 360 degree Specimen holder Spring Loaded stage clips 6 Condenser Type Flip Top Abbe condenser daylight filter detachable N A 1 25 Aperture iris diaphragm Built in 7 Dimensions Weight 405mm L x 210m...

Page 32: ...09 LABOMED and Lx POL are registered trademarks of Labo America Inc With a policy of continuous development Labo America Inc reserves the right to change design and specifications without prior notice...

Reviews: