Chapter 4: Operating Instructions
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Note:
The system will go from room temperature start up to –55°C in
under 7 hours.
5.
Shelf temperatures and vacuum set points can be changed at anytime
during the manual Freeze Dry process.
Manual Mode with Unfrozen Samples
1.
With the display set in manual, select “Set Point Temperature” = Pre-
Freeze.
2.
Set mode to MAN.
3.
Place unfrozen samples on the shelf and close the door. The sample
probe can be placed in the sample.
4.
Start the refrigeration system by pressing “RUN/STOP.”
5.
Start the vacuum pump by pressing “VAC.” Let the pump run 10
seconds. If the pump is allowed to run too long, sample may be lost.
6.
The shelf temperature will cool to approximately –75°C and freeze
the sample.
Note:
Starting the system at room temperature will take approximately 6
hours to freeze a 2 liter tray of water.
7.
When the sample is frozen change the shelf “Set Point Temperature”
to the desired temperature between –55°C and 50°C.
8.
Turn on vacuum.
9.
Shelf temperatures and vacuum set points can be changed at anytime
during the manual freeze dry process.
Auto Mode with Pre-Frozen Samples
1.
With the display set to Auto, program the desired ramp, temperature
and hold times for each segment of the program. The “PREFREEZE”
segment time should be set to 0. This allows the program to start at
segment 1.
2.
Set mode to AUTO.
3.
Start the refrigeration system by pressing “RUN/STOP.” Start the
vacuum pump.
4.
When the collector temperature reaches –80°C and the shelf
temperature reaches the desired set point of segment 1, turn off the
vacuum pump and release the vacuum. Place the frozen samples on
the shelf. Close the door and turn the vacuum on.
Auto Mode with Unfrozen Samples
1.
With the display set to Auto, program the desired ramp, temperature
and hold times for each segment of the program. The “PREFREEZE”
segment time must be set between 3 hours and “INDEF.” This allows
the shelf to cool and pre-freeze the sample. The samples must be
completely frozen during “PRE FREEZE” or samples will be
