Kurabo QG-Auto12S Operation Manual Download Page 1

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Automated Nucleic Acid Isolation Device

QuickGene-Auto12S
QuickGene-Auto24S

Ver. 1.0

Operation Manual

Summary of Contents for QG-Auto12S

Page 1: ...Automated Nucleic Acid Isolation Device QuickGene Auto12S QuickGene Auto24S Ver 1 0 Operation Manual ...

Page 2: ......

Page 3: ...al control Disclaimer The contents of this document are subject to change without prior notification Our company shall not be liable for Any violation of patent right of a third party or other rights through use of data described in this document Any failure or damage caused by installation relocation remodeling maintenance or repair carried out by any party other than our company or other than a ...

Page 4: ...prohibited actions Note Note indicates precautions instructions to abide by supplemental explanations etc Intended Use QuickGene Auto12S 24S which collocate with related reagent perform automated isolation of nucleic acid from biological sample The sample derived from human beings could be different kind of sample such as blood tissue plasma interstitial fluid which need to be well pretreatment th...

Page 5: ...g terminal Do not handle the power plug with wet hands Do not use with a voltage other than the indicated voltage and avoid branch connections or extension cords Do not use with a damaged power plug or a loose socket If there is dust on the prongs of the power plug or on the plug socket remove it with a dry cloth When you disconnect the plug from the outlet be sure to hold the power plug itself Do...

Page 6: ...t ride above the device Falling and injury may occur Wear protective gloves when replacing consumables If ethyl alcohol is spilled promptly remove power plug from outlet Repairs to QG Auto12S 24S should only be performed by such agencies as are specifically authorized by KURABO Industries Ltd Only original KURABO Industries Ltd replacement parts should be used Instruction for handling hazardous su...

Page 7: ...Reagent Kit QuickGene AutoS RNA Cultured Cell kit AS RC NB These products are not included in this system package Please purchase separately Reagents Used for Other than Dedicated Kits For reagents used for other than a dedicated kit the work should be carried out in accordance with the safety precautions for handling and use of each reagent Use in a laboratory or work space suitable for isolation...

Page 8: ...et light Warning Labels Warning caution instruction and obligation labels are affixed to this system Wear protective equipment QG Auto12S 24S may become contaminated with infectious substances etc When inserting your hands into the device be sure to wear protective gloves Read operation manual Read and understand operation manual before using this equipment Biohazard Risk Label This label indicate...

Page 9: ...ings Mark Description Mark Description Manufacturer information Consult instructions for use SN Serial number European Authorized Representative Do not dispose of this product as unsorted municipal waste Humidity limitation Caution Consult accompanying documents Temperature limitation Recyclable materials ...

Page 10: ...e of QG Auto12S 24S and QuickGene Consumables No liability is assumed for the results regardless of the purpose of use Validation of performance on samples shall be based on the judgment and responsibility of the user When using this device please thoroughly read this document and abide by the safety rules of your facility ...

Page 11: ...5 Mounting of UV Lamp 2 How to Operate p 11 2 1 Confirmation of Articles to be Prepared 2 2 Preparation of Reagents 2 3 Preparation for Operation 2 4 Operation of DNA RNA Isolation 2 5 Collection of Isolation Samples 2 6 After Operation 3 Cleaning and Maintenance p 25 3 1 Daily Maintenance 3 2 Weekly Maintenance 3 3 Half a Year Maintenance 3 4 How to replace the power fuse 4 Before Concluding as a...

Page 12: ...x QuickGene Auto12S QuickGene Auto24S Operation Manual ...

Page 13: ...are explained The isolation operation is explained The procedures for cleaning and maintenance are explained The handling procedures when you consider a failure has occurred are explained 1 2 3 4 1 Installation Procedures 2 How to Operate 3 Cleaning and Maintenance 4 Before Concluding as a Failure ...

Page 14: ...xii QuickGene Auto12S QuickGene Auto24S Operation Manual ...

Page 15: ...ing curtains or blinds as necessary A location which is well ventilated and not dusty A location with minimal sudden temperature fluctuation Warming a cold room suddenly or moving the device from a room with low temperature to a warm room may cause condensation inside the device resulting in sub standard performance A location where the temperature and humidity are kept within the specified range ...

Page 16: ...lease install this device in an indoor location as shown below for normal safe use Required footprint Width 68 cm Depth 82 cm Height 79 cm Installation location of QuickGene Auto24S Please install this device in an indoor location as shown below for normal safe use Required footprint Width 103 cm Depth 82 cm Height 80 cm 80 cm ...

Page 17: ...rce LED white light Heatingblock 25 60 C User s interface 7 TFT LCD touch screen 800 400 Operating environment Item Specification Temperature 15 30 C Humidity 30 80 relative humidity non condensing Input power 100 240 V 50 60Hz Overvoltage category definition Transient overvoltage category II Table tolerance weight QuickGene Auto12S 55 kg QuickGene Auto24S 80 kg Operating parameters Item Specifica...

Page 18: ... alone 2 or more people are needed for carrying QuickGene Auto12S 4 or more people are needed for carrying QuickGene Auto24S Follow the instructions to unpack the device 1 2 1 Remove the outer carton four foam pieces and take out the device Wooden carton dimension QuickGene Auto12S 71 x 89 x 84 cm QuickGene Auto24S 105 x 95 x 93 cm Caution ...

Page 19: ...gent holder and syringe head from inside the instrument and cut the cable tie of Reagent holder 1 2 3 Remove the foam between the Y2 axis and worktable from inside the instrument and cut the cable tie of Collection holder 1 2 4 Take out the Collection holder remove the wing screw and take out the UV lamp then install it ...

Page 20: ...o24S Operation Manual 1 1 2 5 Plug in the Power Cable and turn on the rear power switch 1 2 6 Turn on the main switch Orange Do not obstruct the mains plug or position the equipment where the connection to the mains line can be accidentally disconnected Warning ...

Page 21: ... carton before using the device If any item is missing please contact your distributor Main Unit Operation Manual Power Cable Reagent Holder Collection Holder O ring Plastic tank UV Lamp Grease Item QuickGene Auto12S QuickGene Auto24S Main Unit 1 1 Operation Manual 1 1 Power Cable 1 1 Reagent Holder 1 2 Collection Holder 1 2 O Ring 12 24 Plastic Tank 3 6 UV lamp 1 1 Grease 1 1 ...

Page 22: ...eration Manual 1 1 4 Device Overview The names of parts and their functions are explained below QuickGene Auto12S Interior View of QuickGene Auto12S Operation panel USB Port Power Switch Front Door Ventilation hole AC inlet Holder guide upper Holder guide under Heater ...

Page 23: ... QuickGene Auto12S QuickGene Auto24S Operation Manual 1 QuickGene Auto24S Interior view of QuickGene Auto24S Operation panel Power Switch Ventilation hole AC inlet USB Port Front Door Heater Holder guide upper Holder guide under ...

Page 24: ...s and the lamp socket inserting position Insert the UV lamp and amount on the lamp socket 1 Insert the UV lamp along the vertical groove of the lamp socket to the end 2 Turn the UV lamp clockwise 90 to mount on the lamp socket Turn until a clicking sound is heard NB To remove the UV lamp follow the reverse order of above Lamp Socket Lamp Socket Side Align the center vertical lamp socket and the me...

Page 25: ...ple until completion of isolation work 2 1 Confirmation of Articles to be Prepared Explanation regarding confirmation of prepared articles before conducting isolation work QuickGene Auto12S or QuickGene Auto24S Main Unit and Accessories Refer to 1 4 Checking of Packed Contents and confirm that all are included Other articles to prepare The following articles shall be prepared NB They are not inclu...

Page 26: ...indicated on the outer box of the reagent kit Reagent strips QuickGene AutoS kits include reagent strips Following things are included each wells of reagent strips 1 2 Short tip 3 Tip pack need to be set 1ml long tip after setting reagent strip into the Reagent holder 4 Nothing need to be set sample tube before starting operation 5 10 Reagents 11 Cartridge with Adopter 12 Elution Buffer Preparatio...

Page 27: ... the waste tube and elution tube in the Collection holder 2 Open the lid of Reagent holder and put the strip into the holder 3 Set 1 ml long tip at the 3 of reagent strips Note Confirm tip packs is correctly set before setting 1 ml long tip 4 Check adaptors are correctly set on the cartridges and close the lid ...

Page 28: ... Note The collection holder need to be set into the holder guide under During the heating block is hot about 50 C please be careful of getting burn due to touch on the heating block Be careful not to hit your head against the front door when setting the holder to the equipment 7 Put the Reagent holder and lock it by four stoppers front and back Note The cartridges should be set at the front side o...

Page 29: ...S Operation Manual 2 8 Prepare the sample and put it into the bottom of Sample Tube then put it into 4 of reagent strip Press it to the end Please refer to the kit handbook for sample preparation If you set inappropriate sample cartridges may clog Warning ...

Page 30: ... is showed up Wait until the initialization screen is displayed 3 Initializing screen is showed up The device proceed through a self check and move to the home position about all moving parts When the heater temperature reached to 25 o C the touch panel shows the Home screen Each parts means as follows S Cylinder operation vertical movement Z Dispenser operation vertical movement Y1 Dispense unit ...

Page 31: ...UE PLASMID CUSTOMIZED PROTOCOL green ISOLATE A E Additional protocol SYSTEM purple Information 5 Choose the elution volume and make sure all the accessories has been putted in the system Confirm whether all consumables are correctly set and complete the check list then Next button will show up 6 Check the protocol information is correct then press the Start button to proceed the isolation Then pro...

Page 32: ...lease press terminate When you select Yes the operation will be terminated and the screen will go back to the Home screen When you select No the operation will be continued and the screen will be returned to the previous one 7 During the running step the touch panel show the processing and remaining time 8 After finishing the protocol the beeper will call press Home button return to Home screen th...

Page 33: ...tton When you press the Pause button you can select Stop or Continue If you press the Continue button the device will continue the running If you press the Stop button warning page will show up At this warning page When you press No the device will continue the protocol and the touch panel will go back to running screen When you press Yes the protocol will be finished After pressing Yes the touch ...

Page 34: ...to operate the Customized protocol 1 Press the CUSTOMIZED PROTOCOL 2 Choose Protocol Refer to the Normalized protocol operation procedure Note When the protocol is not installed the following page will be showed up If you need customized protocol please contact to your distributor ...

Page 35: ... Auto24S Operation Manual 2 How to use the System purple 1 Press the SYSTEM 2 Operation menu will be showed up Information You can check Model name serial number and software version will be showed up DEMO run Used by manufacturers and distributors ...

Page 36: ...e front door has been closed the Collection holder and the Reagent holder has been removed 1 Press OK to start UV irradiation 2 UV irradiation will start and continue for 5 minutes 3 Press Pause button to stop UV irradiation 4 Press Stop if you really want to terminate UV irradiation Press Continue if you want to continue UV irradiation Warning ...

Page 37: ...kGene Auto24S Operation Manual 2 Delivery mode Used by manufacturers and distributors As usual if you proceed the operation this screen will be showed up Devices test Used by manufacturers and distributors This function is only for engineer use ...

Page 38: ... Note The isolation sample may be spilt if the collection tube holder is tilted Take out while holding the handle of the collection tube holder with one hand and supporting the bottom surface of the holder with the other hand 2 Securely close the cap of microtubes collection tubes on Collection tube holder 3 Take out the Collection holder from the device 4 Collect the microtubes collection tubes ...

Page 39: ... tubes from the Collection holder and dispose of waste fluid and waste tubes Waste fluid and waste tubes should be disposed in accordance with customer s infectious waste treatment manuals Post Treatment of Device Refer to 3 Cleaning and Maintenance and carry out system maintenance as necessary 1 Close the front door The isolating operation is now completed When the operation is to be continued st...

Page 40: ...lution 2 chlorine bleach Water Use distilled or deionized water Strong detergents may dissolve carrier and worktable surface coatings Use only recommended cleaning agents Chlorine solution can pit equipment and metal Use adequate amounts or repeated applications of 70 ethanol or water until chlorine residue is no longer visible II Decontamination Procedure using 0 1 Sodium Hypochlorite A Between d...

Page 41: ...t holder washes with water and air dry ii If Collection holder has been contaminated loosen the wing screw of Plastic tank and take it off Wash Collection holder with water and air dry then replace the new Plastic tank and tight the screw Notice the direction Front For Waste Tube iii Clean system worktable ...

Page 42: ...Please refer to page 18 i At Home screen select System ii At System screen select UV lamp iii Make sure the front door already has been closed Press OK button to start the UV irradiation iv If it is necessary you can press Pause button 3 3 Half a Year Maintenance It is recommended to replace O ring every half a year It may be changed up to your frequency of use i Use a sharp blade to cut off old O...

Page 43: ...placing the fuse Please check whether AC inlet is broken and replace it if necessary i Please take out the fuse holder from AC inlet If this time is the first time changing then it will two pcs of fuse inside it One is in use the other one is for back up ii Take out the in use broken one fuse and replace the new one iii Make sure the fuse is installed in the middle Two side silver parts red frame ...

Page 44: ...tenance 29 QuickGene Auto12S QuickGene Auto24S Operation Manual 3 iv Insert the fuse holder to AC inlet Ensure the direction is correct If it is available Please prepare spare fuse for further used Specification AC250V 5A ...

Page 45: ...3 Cleaning and Maintenance 30 QuickGene Auto12S QuickGene Auto24S Operation Manual 3 ...

Page 46: ...ical service UV lamp is not working 1 UV lamp was installed incorrect 2 The wire connect to the UV starter was not inserted well 3 Door is not closed or UV sensor is not sensed 1 Follow operation manual and re install 2 Contact to local distributor 3 Close the door and check the UV sensor LED light is not working The wire connect to the TFT board was not inserted well or broken Contact to local di...

Page 47: ...e install 2 Follow operation manual and put it in the right position 3 Contact to local distributor Elution tube is empty 1 Plastic tank is not installed correctly 2 Is waste tube and elution tube put well 3 Y2 axis motor is not working 1 Follow operation manual and re install 2 Follow operation manual and put it in the right position 3 Contact to local distributor Machine stop when it is performi...

Page 48: ...nter 3F 14 5 Shimokida cho Neyagawa Osaka 572 0823 Printed in Japan The contents of this document are subject to change without prior notification Kurabo Industries Ltd is not liable for violation of any patent rights or other rights of a third party caused by the use of data described in this document All rights are reserved Any defective issues with missing or disarranged pages will be rectified...

Page 49: ...S LTD Doc ID 602656 PSQPSBUF 0GGJDF 4 05 OD SWJOHUPO 3PBE 0NBIB 64 1IPOF BY POUBDU OGPSNBUJPO 4 JPUFD JNJUFE 8BUU 3PBE JMMJOHUPO 1BSL MBTHPX 3 6 3FHJTUFSFE JO OHMBOE BOE 8BMFT 1IPOF BY NBJM JOGP BETCJPUFD DPN XFC XXX BETCJPUFD DPN ...

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